Ccdc36 (NM_001135198) Mouse Untagged Clone

CAT#: MC219250

Ccdc36 (untagged) - Mouse coiled-coil domain containing 36 (Ccdc36), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ccdc36
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219250 representing NM_001135198
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTTTAATGTCTGGAATGTTAAAGAGATGCTCAGCATCCCCTCAGGCTCTGGGATCACTAAACCAT
  CTAACTGGAATAATAATCAGACTGACTGTTCTCTCAGTGATTCCCAGTTCCTCTTTGGATCTCAGTTCTG
  TCCTGAAAATTCAGAGACTCTGCTACCGTCCTTGGATGCTGGTGCTTGCTTAAGACACCCAAAACAGACA
  CAACAGAATTCTGTGGATAGTGAACCTAGTATTTTCATAAAATACCAGGCAAAGCCCCAGTTGCTTGGAG
  GCGATACAAAAGATGAAAGCTTATTTTCTCTTCCTTTGCCAGTTGGGAAATCAAAAGGACTCTCAAAACA
  GTTTGAGGAGAAAAAACGAAGGGCAACGGACCAATCTGACAGTGAGACCCTGCACAGCTTTGTTTCTCAT
  TTTCCAGAAGTCATTAACAAGTTGCAGACATCTGTGGAAAAGACTGAGGAAAATCTCAGCTCTAGAAGCC
  AGTCTATTTTAGATTCTGTGGAGACCATAGCCAAGACTTTTCAAGAGACTGCACGGGTCCAGCACGACCT
  GATGGTTGAGTCAGTGAGAGACAAAGGCAGCATGGAGCAGGCCATCCTTGAGATCCAGCGGACATGTGCA
  GCTAGACAAGCAGAATTTATGGAGATGAAATCCACCCTGAAGAACCTTGAAGTTTTAGTTGTGGAGCAGA
  CTAAGAACTTGCAGCAGTTTTGTGACAACCTGAGCCAGCTCATTGTGCCTGGTATCCTAGAAGAGCTGAA
  AAAATTCACCTCTGTGCCTCAGGTGGCTGGGCATCTGAAAGACAGCACCTCTCAGACCTCACCATCTCTG
  ACCCAGAGCCTCCATTTTACCAGGCAGGAAAAGCATCCCTCTGAGGAGCCAGCTACTTGGCAGGCCCAGG
  AGGCTCCTGCAGGAAATCCCAGTACGAGTTCCCAGAGGCCTGGGGAGTGTGGTGTCTGGGATGAAGGAGC
  AGAGAGTGGTGTTTTTCAAAAGGCTGCATTGCCAACAGATGGGCTCCATAGAGGCGATGGGCATGTAAAG
  AACAAGACCGTGCCGACTTACTGCAAGAACTGGGTCATGACCACCAGAAGCGTCAGTAACCACTTCTCTA
  ACCTCCCAAGCCAGAGGGCTGGCAATGGCCAAGGTCTGATGGCCCAAGGAGCTTCACAGCGGGACGTAAG
  TAAGTTTGAAGCCAGAGTGAAGAATGCCTGCCCCGAATATGGGCCCCAAAGCATGTGTTCTTTTGACTCA
  CTTGAACAGTCAGCAACCGAACAGAAAGGCAGGCCCTGCAGAAAAAGGAGGAGAGGCAAGAAGCAACAAC
  CCCAGAGATCAAAGAGAGGTGGGCTTCTAGATAGGAAGCAAGGACAGACCTCAAAGGCGGCCTGTGCTTT
  CATAGCTAGGCATCACTGCCCCCAGTCTCCAGTGTGTGATCCACAGGGGCCCCTCATCTGTTGGCTGACT
  CCGAGAAGTTCCACAAAGTCAACCTGTCACATTTTGGGAGGAACGGGAGAGACCAGTCAGACAGCAAGGG
  CAGCACAAGGGAACCTCGTGCAGCATTCCCAGCGCTCTTCCACAGACAGCAGCTCCCAGGGAGACCAGCA
  GATAAATTGGTTCAGTGACCTCAGCCTTGAAAACCTAGAGCCGCCACAGTGTAAGAAGGGAGGGACGAAT
  TTGCTCTGTGACCCGGATTTTGATAGCAGCGATGATAATTTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001135198
• Insert Size: 1725 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001135198.1, NP_001128670.1
• RefSeq Size: 2878 bp
• RefSeq ORF: 1725 bp
• Locus ID: 434438
• UniProt ID: Q6PDM4
• Cytogenetics: 9 F2
• Gene Summary: Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination (PubMed:27723721). Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis (PubMed:27723721). Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance (PubMed:27723721).[UniProtKB/Swiss-Prot Function]