Syncrip (NM_019796) Mouse Tagged ORF Clone

CAT#: MR218806

Syncrip (Myc-DDK-tagged) - Mouse synaptotagmin binding, cytoplasmic RNA interacting protein (Syncrip), transcript variant 2

Product Images

Circular map for MR218806

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Syncrip
• Synonyms: 2610109K23Rik; 4632417O19Rik; GRY-RBP; hnRNP Q; Nsap1; Nsap1l; pp68
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218806 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACAGAACATGTTAATGGAAATGGTACTGAAGAGCCCATGGATACTACTTCAGCAGTTATCCATT
  CAGAAAATTTTCAGACATTGCTTGATGCTGGTTTACCACAGAAAGTTGCTGAAAAACTAGATGAAATTTA
  CGTTGCAGGGCTAGTTGCACATAGTGATTTAGATGAAAGAGCTATCGAAGCTTTAAAAGAGTTCAATGAA
  GACGGCGCATTGGCAGTGCTTCAACAGTTTAAAGACAGTGATCTCTCTCATGTTCAGAACAAAAGTGCCT
  TTTTATGTGGAGTCATGAAGACTTACAGGCAGAGAGAAAAACAGGGGACCAAAGTAGCAGACTCTAGTAA
  AGGACCAGATGAGGCAAAGATTAAGGCACTTTTGGAAAGAACAGGCTACACACTTGATGTGACTACAGGT
  CAGAGGAAGTATGGAGGACCACCTCCAGATTCCGTTTATTCAGGTCAGCAGCCTTCTGTTGGCACTGAGA
  TATTTGTGGGGAAGATCCCCAGAGATCTGTTTGAGGATGAGCTTGTTCCATTATTTGAGAAAGCTGGACC
  TATATGGGATCTTCGTTTAACGATGGATCCGCTCACTGGTCTCAACAGAGGTTATGCGTTTGTCACTTTT
  TGTACAAAAGAAGCAGCACAAGAGGCTGTTAAACTGTATAATAATCATGAAATTCGTTCCGGGAAGCACA
  TTGGTGTCTGCATCTCAGTTGCCAACAATAGGCTTTTTGTGGGCTCGATTCCTAAGAGTAAAACCAAGGA
  GCAGATTCTTGAGGAATTTAGCAAAGTGACAGAGGGTCTCACAGATGTCATTTTATACCACCAACCTGAT
  GACAAGAAAAAAAACAGGGGCTTTTGCTTTCTTGAATATGAAGATCACAAAACAGCTGCCCAGGCAAGAC
  GTAGGCTAATGAGTGGTAAAGTCAAAGTCTGGGGAAATGTTGGAACTGTTGAGTGGGCTGATCCTATTGA
  AGATCCTGATCCTGAAGTTATGGCAAAGGTAAAAGTGCTGTTTGTACGCAACCTTGCCAACACGGTAACA
  GAAGAAATTTTAGAAAAGTCATTTAGTCAGTTTGGGAAACTGGAACGAGTAAAGAAGCTAAAAGATTATG
  CTTTCATTCATTTTGATGAGAGAGATGGTGCTGTCAAGGCTATGGAAGAAATGAATGGTAAAGACTTGGA
  GGGAGAAAATATTGAAATTGTTTTTGCTAAGCCACCAGATCAGAAGAGGAAAGAAAGAAAAGCTCAGAGG
  CAAGCAGCAAAGAATCAAATGTATGATGATTACTACTATTATGGTCCACCTCATATGCCTCCCCCAACAA
  GAGGTCGAGGGCGTGGAGGTAGAGGTGGCTATGGATATCCTCCAGATTATTATGGATACGAAGATTATTA
  TGATTATTATGGTTATGATTACCATAACTATCGTGGTGGATATGAAGATCCATACTATGGTTATGAAGAT
  TTTCAAGTTGGAGCTAGAGGAAGGGGTGGTAGAGGAGCAAGGGGTGCTGCTCCATCCAGAGGTCGTGGGG
  CTGCTCCTCCCCGTGGTAGAGCCGGTTATTCACAGAGAGGAGGCCCTGGATCAGCAAGAGGCGTTCGCGG
  TGCGAGAGGAGGTGCCCAACAACAAAGAGGCCGCGGGCAGGGAAAAGGGGTCGAGGCCGGTCCTGACCTG
  TTACAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218806 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATEHVNGNGTEEPMDTTSAVIHSENFQTLLDAGLPQKVAEKLDEIYVAGLVAHSDLDERAIEALKEFNE
  DGALAVLQQFKDSDLSHVQNKSAFLCGVMKTYRQREKQGTKVADSSKGPDEAKIKALLERTGYTLDVTTG
  QRKYGGPPPDSVYSGQQPSVGTEIFVGKIPRDLFEDELVPLFEKAGPIWDLRLTMDPLTGLNRGYAFVTF
  CTKEAAQEAVKLYNNHEIRSGKHIGVCISVANNRLFVGSIPKSKTKEQILEEFSKVTEGLTDVILYHQPD
  DKKKNRGFCFLEYEDHKTAAQARRRLMSGKVKVWGNVGTVEWADPIEDPDPEVMAKVKVLFVRNLANTVT
  EEILEKSFSQFGKLERVKKLKDYAFIHFDERDGAVKAMEEMNGKDLEGENIEIVFAKPPDQKRKERKAQR
  QAAKNQMYDDYYYYGPPHMPPPTRGRGRGGRGGYGYPPDYYGYEDYYDYYGYDYHNYRGGYEDPYYGYED
  FQVGARGRGGRGARGAAPSRGRGAAPPRGRAGYSQRGGPGSARGVRGARGGAQQQRGRGQGKGVEAGPDL
  LQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019796
• ORF Size: 1689 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019796.1, NM_019796.2, NM_019796.3, NM_019796.4, NM_019796.5, NP_062770.1
• RefSeq Size: 6933 bp
• RefSeq ORF: 1689 bp
• Locus ID: 56403
• UniProt ID: Q7TMK9
• Cytogenetics: 9 E3.1
• MW: 62.6 kDa
• Gene Summary: Heterogeneous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD-mediated complex that promotes MYC mRNA stability. Isoform 1 and isoform 2 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences (By similarity). Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself (By similarity). May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain (By similarity). Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 2 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins.[UniProtKB/Swiss-Prot Function]