Syncrip (NM_019796) Mouse Untagged Clone

CAT#: MC219075

Syncrip (untagged) - Mouse synaptotagmin binding, cytoplasmic RNA interacting protein (Syncrip), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Syncrip
• Synonyms: 2610109K23Rik; 4632417O19Rik; GRY-RBP; hnRNP Q; Nsap1; Nsap1l; pp68
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219075 representing NM_019796
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACAGAACATGTTAATGGAAATGGTACTGAAGAGCCCATGGATACTACTTCAGCAGTTATCCATT
  CAGAAAATTTTCAGACATTGCTTGATGCTGGTTTACCACAGAAAGTTGCTGAAAAACTAGATGAAATTTA
  CGTTGCAGGGCTAGTTGCACATAGTGATTTAGATGAAAGAGCTATCGAAGCTTTAAAAGAGTTCAATGAA
  GACGGCGCATTGGCAGTGCTTCAACAGTTTAAAGACAGTGATCTCTCTCATGTTCAGAACAAAAGTGCCT
  TTTTATGTGGAGTCATGAAGACTTACAGGCAGAGAGAAAAACAGGGGACCAAAGTAGCAGACTCTAGTAA
  AGGACCAGATGAGGCAAAGATTAAGGCACTTTTGGAAAGAACAGGCTACACACTTGATGTGACTACAGGT
  CAGAGGAAGTATGGAGGACCACCTCCAGATTCCGTTTATTCAGGTCAGCAGCCTTCTGTTGGCACTGAGA
  TATTTGTGGGGAAGATCCCCAGAGATCTGTTTGAGGATGAGCTTGTTCCATTATTTGAGAAAGCTGGACC
  TATATGGGATCTTCGTTTAATGATGGATCCGCTCACTGGTCTCAACAGAGGTTATGCGTTTGTCACTTTT
  TGTACAAAAGAAGCAGCACAAGAGGCTGTTAAACTGTATAATAATCATGAAATTCGTTCCGGGAAGCACA
  TTGGTGTCTGCATCTCAGTTGCCAACAATAGGCTTTTTGTGGGCTCGATTCCTAAGAGTAAAACCAAGGA
  GCAGATTCTTGAGGAATTTAGCAAAGTGACAGAGGGTCTCACAGATGTCATTTTATACCACCAACCTGAT
  GACAAGAAAAAAAACAGAGGCTTTTGCTTTCTTGAATATGAAGATCACAAAACAGCTGCCCAGGCAAGAC
  GTAGGCTAATGAGTGGTAAAGTCAAAGTCTGGGGAAATGTTGGAACTGTTGAGTGGGCTGATCCTATTGA
  AGATCCTGATCCTGAAGTTATGGCAAAGGTAAAAGTGCTGTTTGTACGCAACCTTGCCAACACGGTAACA
  GAAGAAATTTTAGAAAAGTCATTTAGTCAGTTTGGGAAACTGGAACGAGTAAAGAAGCTAAAAGATTATG
  CTTTCATTCATTTTGATGAGAGAGATGGTGCTGTCAAGGCTATGGAAGAAATGAATGGTAAAGACTTGGA
  GGGAGAAAATATTGAAATTGTTTTTGCTAAGCCACCAGATCAGAAGAGGAAAGAAAGAAAAGCTCAGAGG
  CAAGCAGCAAAGAATCAAATGTATGATGATTACTACTATTATGGTCCACCTCATATGCCTCCCCCAACAA
  GAGGTCGAGGGCGTGGAGGTAGAGGTGGCTATGGATATCCTCCAGATTATTATGGATACGAAGATTATTA
  TGATTATTATGGTTATGATTACCATAACTATCGTGGTGGATATGAAGATCCATACTATGGTTATGAAGAT
  TTTCAAGTTGGAGCTAGAGGAAGGGGTGGTAGAGGAGCAAGGGGTGCTGCTCCATCCAGAGGTCGTGGGG
  CTGCTCCTCCCCGTGGTAGAGCCGGTTATTCACAGAGAGGAGGCCCTGGATCAGCAAGAGGCGTTCGCGG
  TGCGAGAGGAGGTGCCCAACAACAAAGAGGCCGCGGGCAGGGAAAAGGGGTCGAGGCCGGTCCTGACCTG
  TTACAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_019796
• Insert Size: 1689 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019796.5, NP_062770.1
• RefSeq Size: 6933 bp
• RefSeq ORF: 1689 bp
• Locus ID: 56403
• UniProt ID: Q7TMK9
• Cytogenetics: 9 E3.1
• Gene Summary: Heterogeneous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD-mediated complex that promotes MYC mRNA stability. Isoform 1 and isoform 2 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences (By similarity). Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself (By similarity). May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain (By similarity). Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 2 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses a distinct 3' terminal exon and differs in the 3' coding region and 3' UTR, compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct C-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.