Stxbp5l (NM_001114613) Mouse Tagged ORF Clone

CAT#: MR218459

Stxbp5l (Myc-DDK-tagged) - Mouse syntaxin binding protein 5-like (Stxbp5l), transcript variant m

Product Images

Circular map for MR218459

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Stxbp5l
• Synonyms: 9430094L02; A830015P08Rik; LLGL4; T2dm1; t2md1; tomosyn-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218459 representing NM_001114613
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAAGTTTAATTTCCGAAAAGTTTTGGATGGCTTAACTGCCTCTTCCCCTGGCAGTGGTAGCAGCA
  GTGGCAGTAACAGTGGTGGGGCTGGAAGTGGCTCTGTCCATCCAGGAGGGACTGCAGGGCTTCCGAGAGA
  GGAAATTCAGGAAAGCCTGACTTCAGACTATTTCCAGATTTGCAAGACAGTTCGGCATGGGTTTCCTTAT
  CAGCCCACAGCATTGGCCTTTGATCCAGTCCAGAAAATCTTAGCTATTGGAACAAGAACAGGTGCTATAC
  GAATACTTGGGAGGCCTGGTGTTGACTGTTACTGCCAACATGAAAGTGGTGCGGCTGTCTTGCAGCTCCA
  ATTCCTCATCAATGAGGGCGCGTTGGTCAGTGCGAGTTCAGATGACACGCTTCATTTGTGGAATCTGAGA
  CAGAAAAGGCCGGCCATTCTCCATTCTCTTAAATTTAACAGAGAACGAATTACTTACTGTCATCTACCTT
  TCCAGAGTAAATGGCTCTATGTTGGAACAGAAAGAGGAAATACACATATTGTAAATATTGAATCTTTCAT
  TCTTTCTGGATATGTCATCATGTGGAACAAGGCAATTGAACTCTCCACCAAGACTCATCCAGGCCCAGTT
  GTACATTTAAGTGATAGCCCAAGAGATGAAGGAAAACTGTTAATAGGTTACGAGAATGGCACTGTGGTGT
  TCTGGGACTTGAAATCTAAAAGAGCAGAACTGAGAGTTTATTATGATGAGGCTATTCATTCAATTGATTG
  GCATCATGAGGGCAAACAATTCATGTGCAGCCACTCAGATGGTAGCTTGACTTTATGGAACCTGAAAAGC
  CCAAGCCGCCCTTTCCAAACCACAGTCCCACATGGAAAAAGTCAAAGAGAAGGAAGAAAGTCTGAGTCTT
  GTAAACCTATTCTTAAAGTAGAATACAAAACGTGCAGGAACAGTGAACCATTTATAATATTCTCTGGCGG
  GCTGTCATATGACAAAGCTTGCAGAAGACCAAGTTTAACCATCATGCATGGGAAAGCAATTACAGTGCTT
  GAGATGGATCATCCTATTGTTGAGTTTCTAACGTTATGTGAAACGCCCTATCCAAATGAATTTCAAGAAC
  CCTATGCTGTTGCTGTACTTCTAGAGAAAGATCTCATCGTAGTTGACCTGACACAAACCAATTTTCCAAT
  CTTTGAAAATCCATATCCAATGGACATTCATGAGTCACCTGTTACATGCACAGCATATTTTGCCGATTGC
  CCTCCAGATTTGATTCTAGTACTCTATTCTATAGGAGTCAAGCATAAAAAACAAGGATACAGTAATAAGG
  AATGGCCAGTCAGTGGAGGAGCTTGGAACCTCGGAGCACAAACTTATCCAGAAATTATTATTACTGGTCA
  TGCTGATGGAACAATAAAATTTTGGGATGCTTCAGCAATGACACTGCAGATGTTGTACAAGCTAAAAACT
  TCCAAAGTGTTTGAAAAGCAGAAGGCAGGAGAAGGCAAACAGACGTGTGAACTTGTAGAGGAAGATCCCT
  TTGCTGTGCAGATGATCTACTGGTGTCCAGAGAGCAGAATATTTTGTGTCTCTGGAGTCTCTGCATATGT
  CATAATTTATAAATTCAGCAGACATGAAGTCACAACAGAAATAGTGTCACTAGAGGTACGACTTCAGTGT
  GATGTTGAAGACATTATTACTCCTGAGCCAGAAACAAGCCCTCCATTTCCAGATCTCTCCTCTCAACTTC
  CTCCTTCAAGGAGTCTTTCTGGAAGCACTAATACTGTGTCTAGTGAAGGAGTGACAAAGGACAGCATCCC
  GTGCCTCAGTGTTAAAACACGGCCAGTACGAATGCCGCCGGGCTATCAAGCAGATCTTGTTATTCAGTTG
  GTGTGGGTAGATGGTGAGCCTCCTCAGCAAATTACCAGCCTTTCTATAAGCTCAGCATATGGAATAGTGG
  CATTTGGAAACTGCACTGGGCTGGTTGTGGTGGATTTCATACAGAAGACAGTGCTATTAAGCATGGGGAC
  CATTGACCTCTATAGATCAAGTGACCTGTACCAGCGACAGCCCCGGTCTCCTCGAAAGAACAGGCAGTTC
  ATTGCAGACAACTTTTGCATGCGTGGGCTGTCTAACTTTTATCCTGATTTAACGAAACGGATCCGTACTT
  CCTATCAGAGCTTAACAGAGCTGAATGATAGTCCAGTTCCCCTGGAACTTGAGCGCTGCAAGTCCCCCAC
  CTCAGAAACCCGAGAGAATTCCTATAATCGCTCCAGGAGCTCTAGCATCTCCAGTATTGACAAAGATTCT
  AAAGAAGCAATTACAGCTTTATACTTCATGGAATCCTTTGCACGGAAAAATGACTCTACCGTTTCCCCTT
  GTCTGTTTGTTGGAACTAGTCTGGGAATGGTAGTACTCATCTCCCTAAATCTACCATCATCAGATGAACA
  AAGGTTTACAGAGCCGGTGGTGGTATTGCCAAGTGGTACATTCCTCTCACTGAAAGGAGCAGTGCTAACA
  TTTTCCTGCATGGACAGAACTGGCAGTTTAATGCAGCCTCCATACGAAGTATGGAGAGATCCAAACAACA
  CAGATGAAAATGAAAAAACCTGGAAAAGGAAATTGGTCATGAACTACTCGTCTTCATCCCAAGAGATGGG
  AGACCATCAGTATACGATAATCTGCTCAGAAAAACAAGCCAAAGTCTTCTCACTGCCTTCCCAGACCTGC
  CTTTATGTTCACAACATCACCGAGACGTCGTTCATATTGCAAGCAGATGTGGTGGTCATGTGCAACAGTG
  CCTGCCTGGCCTGCTTTTGTGCAAATGGGCATATCATGATAATGAGCTTACCTAGTCTTCGCCCAATGTT
  GGATGTTAATTATTTGCCACTGACAGACATGAGAATAGCACGGACATTTTGTTTTACTAATGAAGGACAG
  GCATTATACCTCGTCTCTCCCACTGAAATTCAACGGTTAACATATAGCCAAGAAATGTGTGATAATATTC
  AGGACATGTTAGGCGATTTGTTTACTCCCATAGAGACACCAGAAGCCCAAAATAGAGGCTTTCTCAAGGG
  ACTATTTGGTGGAAGTGGACAGACATTCGACAGAGAAGAACTCTTCGGGGAAGCCTCAGCAGGAAAAGCA
  TCCCGAAGCCTCGCACAGCACATCCCTGGGCCAGGTAGTATAGAAGGGATGAAAGGTGCTGCTGGCGGGG
  TGATGGGAGAGCTGACCCGAGCCCGGATTGCACTTGATGAGAGAGGGCAGCGTCTGGGCGAGCTGGAAGA
  GAAGACTGCGGGCATGATGACCAGCGCAGAAGCGTTTTCCAAGCACGCACATGAGCTAATGCTGAAGTAT
  AAGGATAAGAAGTGGTACCAGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218459 representing NM_001114613
  Red=Cloning site Green=Tags(s)
  
  MKKFNFRKVLDGLTASSPGSGSSSGSNSGGAGSGSVHPGGTAGLPREEIQESLTSDYFQICKTVRHGFPY
  QPTALAFDPVQKILAIGTRTGAIRILGRPGVDCYCQHESGAAVLQLQFLINEGALVSASSDDTLHLWNLR
  QKRPAILHSLKFNRERITYCHLPFQSKWLYVGTERGNTHIVNIESFILSGYVIMWNKAIELSTKTHPGPV
  VHLSDSPRDEGKLLIGYENGTVVFWDLKSKRAELRVYYDEAIHSIDWHHEGKQFMCSHSDGSLTLWNLKS
  PSRPFQTTVPHGKSQREGRKSESCKPILKVEYKTCRNSEPFIIFSGGLSYDKACRRPSLTIMHGKAITVL
  EMDHPIVEFLTLCETPYPNEFQEPYAVAVLLEKDLIVVDLTQTNFPIFENPYPMDIHESPVTCTAYFADC
  PPDLILVLYSIGVKHKKQGYSNKEWPVSGGAWNLGAQTYPEIIITGHADGTIKFWDASAMTLQMLYKLKT
  SKVFEKQKAGEGKQTCELVEEDPFAVQMIYWCPESRIFCVSGVSAYVIIYKFSRHEVTTEIVSLEVRLQC
  DVEDIITPEPETSPPFPDLSSQLPPSRSLSGSTNTVSSEGVTKDSIPCLSVKTRPVRMPPGYQADLVIQL
  VWVDGEPPQQITSLSISSAYGIVAFGNCTGLVVVDFIQKTVLLSMGTIDLYRSSDLYQRQPRSPRKNRQF
  IADNFCMRGLSNFYPDLTKRIRTSYQSLTELNDSPVPLELERCKSPTSETRENSYNRSRSSSISSIDKDS
  KEAITALYFMESFARKNDSTVSPCLFVGTSLGMVVLISLNLPSSDEQRFTEPVVVLPSGTFLSLKGAVLT
  FSCMDRTGSLMQPPYEVWRDPNNTDENEKTWKRKLVMNYSSSSQEMGDHQYTIICSEKQAKVFSLPSQTC
  LYVHNITETSFILQADVVVMCNSACLACFCANGHIMIMSLPSLRPMLDVNYLPLTDMRIARTFCFTNEGQ
  ALYLVSPTEIQRLTYSQEMCDNIQDMLGDLFTPIETPEAQNRGFLKGLFGGSGQTFDREELFGEASAGKA
  SRSLAQHIPGPGSIEGMKGAAGGVMGELTRARIALDERGQRLGELEEKTAGMMTSAEAFSKHAHELMLKY
  KDKKWYQF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001114613
• ORF Size: 3384 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001114613.1, NP_001108085.1
• RefSeq Size: 11893 bp
• RefSeq ORF: 3387 bp
• Locus ID: 207227
• UniProt ID: Q5DQR4
• Cytogenetics: 16 B3
• MW: 126.3 kDa
• Gene Summary: Plays a role in vesicle trafficking and exocytosis inhibition (PubMed:25002582). In pancreatic beta-cells, inhibits insulin secretion probably by interacting with and regulating STX1A and STX4, key t-SNARE proteins involved in the fusion of insulin granules to the plasma membrane (PubMed:21998599). Plays also a role in neurotransmitter release by inhibiting basal acetylcholine release from axon terminals and by preventing synaptic fatigue upon repetitive stimulation (PubMed:24744148). Promotes as well axonal outgrowth (By similarity).[UniProtKB/Swiss-Prot Function]