Wdr62 (NM_146186) Mouse Tagged ORF Clone

CAT#: MR216149

Wdr62 (Myc-DDK-tagged) - Mouse WD repeat domain 62 (Wdr62)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Wdr62
• Synonyms: 2310038K02Rik; b2b1508Clo
• Vector: pCMV6-Entry2
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216149 representing NM_146186
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGCTGCCTTAGCGGCCGGAGGTTATACGCGGAGTGACACGATAGAAAAGCTGTCCTCTGTCATGG
  CGGGAGTTCCGGCGCGGAGAAACCAGTCCTCCCCGCCTCCTGCCCCACCGCTCTGCCTCCGGCGGCGGAC
  GCGACTCGCGGCGGCTCCCGAGGACACTGTGCAGAACCGGGTGACACTTGAGAAGGTGCTTGGCATCACA
  GCCCAGAACAGCAGCGGGCTAACCTGTGACCCTGGCACAGGCCATGTGGCCTACTTAGCAGGGTGCGTGG
  TGGTGGTCTTGAACCCCAAGGAGAACAAGCAGCAGCATATATTTAACACAACCAGGAAGTCCCTGAGTGC
  TCTGGCCTTCTCCCCAGATGGGAAGTACATAGTGACAGGAGAGAATGGACACCGGCCAGCTGTGCGCATC
  TGGGATGTGGAGGAGAAGACTCAAGTGGCAGAGATGCTGGGCCACAAGTATGGTGTGGCCTGTGTGGCTT
  TCTCACCCAATATGAAGCACATCGTGTCCATGGGCTACCAACATGACATGGTCCTCAATGTTTGGGATTG
  GAAGAAAGACATTGTGGTGGCTTCCAACAAGGTGTCATGTAGAGTCATCGCTCTCTCCTTCTCTGAGGAC
  AGCAGCTATTTTGTCACCGTTGGGAATCGGCATGTGAGGTTTTGGTTCTTAGAAGCCTCTACTGAGGCCA
  AGGTAACCAGCACAGTGCCCCTGGTAGGACGCTCAGGCATCCTGGGTGAGCTGCACAACAATATCTTTTG
  TGGCGTGGCCTGCGGCCGGGGCCGGATGGCAGGCAATACCTTCTGTGTGTCCTACTCTGGCCTCCTCTGC
  CAGTTCAATGAGAAAAGGGTGCTGGACAAGTGGATCAACCTGAAGGTCTCCTTGTCTTCCTGCCTGTGTG
  TCAGTGACGAGTTGATCTTCTGTGGATGCACAGACGGGATAGTCCGCATCTTCCAGGCCCACAGCCTACT
  CTACCTCACCAACCTGCCCAAACCACACTACCTGGGAGTGGACGTGGCCCACGGACTGGACTCCAGCTTC
  CTCTTCCACAGAAAAGCAGAAGCAGTCTACCCAGATACAGTGGCCCTGACCTTTGACCCCGTCCACCAGT
  GGCTGTCCTGTGTTTACAAAGACCACAGCATCTACATCTGGGATGTCAAGGACATTGATGAAGTCAGCAA
  GATATGGTCAGAGCTCTTCCACAGCTCCTTTGTCTGGAATGTAGAGGTGTACCCTGAATTTGAAGACCAG
  AGAGCTTGCCTGCCGTCCGGGACTTTTCTGACTTGTTCCTCAGACAATACCATCCGCTTCTGGAATTTGG
  ATAGCGCCTCTGACACTCGATGGCAAAAGAACATCTTCAGCGATTCTCTGCTGAAGGTGGTCTATGTAGA
  GAATGACATCCAGCACCTGCAGGACCTGTCTCACTTCCCAGACCGGGGCAGTGAGAACGGCACTCCCATG
  GATATGAAAGCTGGGGTTCGAGTCATGCAGGTCAGTCCTGACGGCCAGCACTTGGCTTCAGGCGACCGCA
  GTGGAAATCTGAGGATCCACGAGCTGCACTTCATGGATGAGCTGATCAAGGTGGAGGCCCACGATGCTGA
  GGTGCTGTGCCTGGAGTACTCCAAGCCTGAGACAGGAGTGACTTTGCTGGCTTCAGCCAGTCGGGACAGA
  CTCATCCATGTGTTAAACGTGGAGAAGAACTACAACCTGGAGCAGACCCTGGACGACCACTCCTCCTCCA
  TCACAGCCATTAAGTTTGCTGGCACCAGAGATGTCCAGATGATCAGTTGTGGAGCTGACAAGAGCATCTA
  CTTTCGCAGTGCCCAGCAGGCCTCGGATGGACTGCACTTTGTCCGTACCCACCACGTAGCAGAGAAGACC
  ACCTTGTATGACATGGATATCGACATCACACAGAAGTATGTGGCAGTGGCCTGCCAGGACCGCAACGTAA
  GGGTCTACAACACCGTGAGTGGGAAACAGAAGAAGTGCTACAAGGGTTCCCAGGGCGACGAAGGGTCCCT
  GCTGAAGGTCCACGTGGACCCCTCAGGCACCTTCCTGGCCACAAGCTGCTCTGACAAAAGCATCTCCTTG
  ATTGACTTTTACTCGGGCGAGTGTGTTGCCAAGATGTTTGGCCATTCGGAAATTGTCACTGGCATGAAGT
  TCACCTACGACTGCCGCCACTTGATCACAGTATCTGGAGACAGCTGTGTGTTCATCTGGCACCTGGGCCC
  GGAGATCACCACCTGCATGAAGCAGCACTTGCTGGAGATCAACCACCAGGAGCAGCAGCAGCAGCCCAAG
  GACCAGAAGTGGAGTGGCCCTCCCAGCCAGGAGACATATGCATCCACACCAAGTGAGATTCGTTCCCTGA
  GCCCTGGAGAGCAGACAGAGGATGAGATGGAGGAGGAATGTGAGCCAGAAGAGTTGCTGAAAACACCATC
  CAAAGACAGCTTGGACCCAGATCCTCGATGCCTGCTGACAAATGGCAAGCTGCCACTCTGGGCAAAGCGG
  CTGCTAGGGGATGATGATGTGGCAGACAGCTCAGCTTTCCACGCCAAGCGCAGCTACCAGCCACATGGCC
  GTTGGGCAGAGCGGGCTGAGCAGGAACCCCTCAAGACCATCCTGGATGCCTGGAGCCTGGATTCCTACTT
  TACACCCATGAAGCCTGAAAATCTCCAAGACTCTGTTCTGGACTCAGTAGAGCCACAGAACCTGGCAGGC
  CTGCTAAGTGAGTGTTCACTCGGTAATGGACACACGTCTCCGGGAGAAGGCTTGGTGAGCTACCTACTTC
  ACCCAGAGTTAGGGAGTCCCAAAGAGGACAACCGAGGCCACCCCTCCTACCTGCCTCTACAGAGGGAAGC
  CACCGAGGCAAGCGAACTCATCCTCTGCTCCCCAGAGGCGGAAGTGTCACTTACAGGGATGCACAGGGAG
  TATTATGAGGAGGAGACAGAGGCAGGACCTGAAGACCAGCAAGGCGACACCTATCTCAGGGTCTCTTCCG
  TCAGTTCAAAGGATCAGAGCCCCCCTGAGGACTCAGGGGAGTCAGAGGCTGAACTGGAGTGCAGCTTTGC
  CGCCGCCCACAGCTCAGCTCCTCAAACGGACCCTGGCCCTCACCTCACCATGACAGCAGGTAAGCCAGAG
  TACCCAAGTACAGAAGAGCTTTCCCAGCCTGAGCTGCCAGGCTTGGGCAATGGCTCCTTACCCCAGACAC
  CTGAGCAGGAGAAGTTCCTCCGCCACCATTTTGAGACACTTACTGATGCCCCTACTGAAGAGCTCTTTCA
  TGGATCCCTGGGAGACATAAAGATCTCAGAGACTGAGGACTATTTCTTCAATCCCCGGCTGAGCATATCC
  ACCCAGTTCCTCTCCCGCCTCCAGAAGACCTCCAGGTGCCCTCCCCGACTGCCCCTGCACCTTATGAAGT
  CTCCAGAGGCTCAGCCTGTGGGCCAAGGGGGCAACCAGCCCAAAGCAGGGCCCCTGAGAGCAGGTACTGG
  CTACATGTCCTCAGATGGGACCAACGTCCTCTCTGGGCAGAAGGCTGAAGAAACTCAAGAGGCCTTGAGT
  CTACTGGACAGGAAGCCTCCAACACCCACATCTGTACTGACCACAGGCCGGGAGCAAAGTATCTCCGCCC
  CATCTTCGTGTTCTTACCTGGAGTCCACAACGAGCTCACATGCCAAGACGACACGCAGCATCTCTCTTGG
  GGACAGTGAGGGCCCTGTGACAGCTGAGCTACCCCAGTCACTTCACAAGCCCTTATCCCCTGGCCAGGAA
  CTCCAAGCCATCCCCACTACAGTGGCACTGACCTCCAGCATCAAAGACCACGAGCCTGCACCGCTTTCCT
  GGGGCAACCATGAGGCCCGAGCCAGCCTGAAACTGACCTTATCCAGTGTCTGTGAGCAGCTGCTCTCTCC
  ACCTCCACAGGAGCCACCCATCACCCATGTGTGGTCTCAGGAACCTGTGGATGTCCCACCTAGTATGGCA
  GTCACAGTAGCCAGCTTCTGTGCACCCAGCCCTGTAGACATGAGCACCCTGGGACTCCACAGTTCTATGT
  TTCTCCCAAAGACCTCAGCCTCTGGGCCCCTAACCCCTCCTGCCCACCTCCAACTTCTAGAGACCAGGTC
  TAGGGTGCCTGGCAGCACTGCTGCTCTCCTGGAGCCCACCCCCGATGCATCAGGTGTGATCGCAGACAGC
  CCTGGACACTGGGACACAGAAGTCCCTACCCCAGAACTCCTAGGCAGTGTGGAATCAGTCTTGCACAGGC
  TGCAGACTGCCTTCCAAGAAGCTCTTGACCTTTACCGCATGCTGGTCTCCAGCAGCCAGCTGGGTCCTGA
  GCAGCAGCAGGCACAGACCGAGCTGGCCTCCACCTTCCACTGGATCCTCAACCAGCTAGAAGCCAGCAAC
  TGTATGGCTGCAGCTAACTTGGCCCCACCCCAGACACTGCCTAGCCCAGACCCTCTGTCCCTACCTACAC
  TTTGTCCCCTGGCCAGCCCAAACCTGCAGGCCCTGCTGGAACACTACTCAGAGTTGCTGGTGCAAGCAGT
  GAGGAGGAAGGCAAGGGGTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216149 representing NM_146186
  Red=Cloning site Green=Tags(s)
  
  MMAALAAGGYTRSDTIEKLSSVMAGVPARRNQSSPPPAPPLCLRRRTRLAAAPEDTVQNRVTLEKVLGIT
  AQNSSGLTCDPGTGHVAYLAGCVVVVLNPKENKQQHIFNTTRKSLSALAFSPDGKYIVTGENGHRPAVRI
  WDVEEKTQVAEMLGHKYGVACVAFSPNMKHIVSMGYQHDMVLNVWDWKKDIVVASNKVSCRVIALSFSED
  SSYFVTVGNRHVRFWFLEASTEAKVTSTVPLVGRSGILGELHNNIFCGVACGRGRMAGNTFCVSYSGLLC
  QFNEKRVLDKWINLKVSLSSCLCVSDELIFCGCTDGIVRIFQAHSLLYLTNLPKPHYLGVDVAHGLDSSF
  LFHRKAEAVYPDTVALTFDPVHQWLSCVYKDHSIYIWDVKDIDEVSKIWSELFHSSFVWNVEVYPEFEDQ
  RACLPSGTFLTCSSDNTIRFWNLDSASDTRWQKNIFSDSLLKVVYVENDIQHLQDLSHFPDRGSENGTPM
  DMKAGVRVMQVSPDGQHLASGDRSGNLRIHELHFMDELIKVEAHDAEVLCLEYSKPETGVTLLASASRDR
  LIHVLNVEKNYNLEQTLDDHSSSITAIKFAGTRDVQMISCGADKSIYFRSAQQASDGLHFVRTHHVAEKT
  TLYDMDIDITQKYVAVACQDRNVRVYNTVSGKQKKCYKGSQGDEGSLLKVHVDPSGTFLATSCSDKSISL
  IDFYSGECVAKMFGHSEIVTGMKFTYDCRHLITVSGDSCVFIWHLGPEITTCMKQHLLEINHQEQQQQPK
  DQKWSGPPSQETYASTPSEIRSLSPGEQTEDEMEEECEPEELLKTPSKDSLDPDPRCLLTNGKLPLWAKR
  LLGDDDVADSSAFHAKRSYQPHGRWAERAEQEPLKTILDAWSLDSYFTPMKPENLQDSVLDSVEPQNLAG
  LLSECSLGNGHTSPGEGLVSYLLHPELGSPKEDNRGHPSYLPLQREATEASELILCSPEAEVSLTGMHRE
  YYEEETEAGPEDQQGDTYLRVSSVSSKDQSPPEDSGESEAELECSFAAAHSSAPQTDPGPHLTMTAGKPE
  YPSTEELSQPELPGLGNGSLPQTPEQEKFLRHHFETLTDAPTEELFHGSLGDIKISETEDYFFNPRLSIS
  TQFLSRLQKTSRCPPRLPLHLMKSPEAQPVGQGGNQPKAGPLRAGTGYMSSDGTNVLSGQKAEETQEALS
  LLDRKPPTPTSVLTTGREQSISAPSSCSYLESTTSSHAKTTRSISLGDSEGPVTAELPQSLHKPLSPGQE
  LQAIPTTVALTSSIKDHEPAPLSWGNHEARASLKLTLSSVCEQLLSPPPQEPPITHVWSQEPVDVPPSMA
  VTVASFCAPSPVDMSTLGLHSSMFLPKTSASGPLTPPAHLQLLETRSRVPGSTAALLEPTPDASGVIADS
  PGHWDTEVPTPELLGSVESVLHRLQTAFQEALDLYRMLVSSSQLGPEQQQAQTELASTFHWILNQLEASN
  CMAAANLAPPQTLPSPDPLSLPTLCPLASPNLQALLEHYSELLVQAVRRKARGD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_146186
• ORF Size: 4572 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146186.3, NP_666298.3
• RefSeq Size: 4744 bp
• RefSeq ORF: 4575 bp
• Locus ID: 233064
• Cytogenetics: 7 B1
• MW: 167.8 kDa