Man2a2 (NM_172903) Mouse Tagged ORF Clone

CAT#: MR215340

Man2a2 (Myc-DDK-tagged) - Mouse mannosidase 2, alpha 2 (Man2a2)

Product Images

Circular map for MR215340

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Man2a2
• Synonyms: 1700052O22Rik; 4931438M07Rik; AI480988; Man IIx; MX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215340 representing NM_172903
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCTGAAAAAGCAGGTGACGGTGTGTGGGGCTGCTATCTTCTGTGTGGCCGTCTTTTCTCTGTACC
  TTATGCTGGACCGAGTGCAGCATGATCCTGCCAGACACCAGAATGGTGGGAACTTCCCCAGGAGCCAAAT
  CTCTGTGCTACAGAACCGGATAGAACAGCTGGAACAACTGCTGGAGGAAAACCATGACATCATAAGCCGT
  ATCAAGGACTCTGTACTGGAACTGACAGCCAATGCGGAGGGCCCGCCAGCCCTGCTGCCCTACCATACCG
  CCAACGGTTCCTGGGCTGTGCTCCCGGAGCCCCGGCCCAGCTTCTTCTCCGTCTCCCCCCAGGACTGCCA
  GTTTGCTTTGGGGGGACGGGGTCAGAAGCCAGAACTACAGATGTTAACCGTGTCTGAGGATTTGCCGTTT
  GACAATGTGGAGGGTGGCGTGTGGAGGCAAGGCTTTGACATCTCCTACAGCCCAAATGACTGGGACACTG
  AAGACCTGCAAGTGTTTGTGGTGCCTCACTCCCACAACGATCCAGGCTGGATCAAGACTTTTGACAAGTA
  CTACACGGAACAGACCCAGCACATCCTCAACAGCATGGTGTCCAAGCTGCAGGAAGACCCCCGGCGGCGC
  TTTCTCTGGGCAGAAGTCTCCTTCTTCGCCAAGTGGTGGGACAACATCAGTGCCCAAAAGAGGGCAGCAG
  TTCGAAGGCTGGTGGGAAATGGGCAGCTGGAGATTGCGACAGGCGGATGGGTGATGCCCGACGAGGCCAA
  CTCCCATTACTTTGCCTTGGTTGACCAGCTCATCGAGGGGCACCAGTGGCTGGAGAGAAACCTGGGTGCA
  ACCCCGCGCTCGGGCTGGGCAGTGGACCCCTTTGGACACAGCTCCACCATGCCTTACCTGCTGCGCCGTG
  CCAACCTGACCAGCATGCTGATTCAGAGGGTGCATTATGCCATCAAGAAGCACTTTGCTGCCACTCATAG
  CCTGGAGTTCATGTGGAGGCAGATGTGGGATTCAGACTCCAGCACAGACATCTTTTGCCACATGATGCCC
  TTCTACAGCTACGACGTTCCACACACCTGTGGCCCCGATCCCAAGATCTGCTGCCAGTTTGATTTCAAAC
  GTCTGCCGGGTGGGAGAATCAATTGCCCTTGGAAGGTGCCACCCCGGGCTATTACAGAAGCCAATGTGGC
  AGACAGGGCAGCCCTGCTCCTGGACCAGTACCGGAAGAAGTCCCGGCTGTTCCGAAGCAACGTCCTCCTC
  GTGCCACTTGGTGATGACTTCCGGTATGACAAGCCCCAGGAATGGGATGCCCAGTTCTTCAACTACCAGC
  GGCTCTTTGACTTCCTCAACAGCAAGCCTGAGTTCCATGTGCAGGCCCAGTTTGGGACCCTCTCTGAGTA
  TTTCGATGCCCTGTATAAGAGGACAGGAGTAGAGCCTGGTGCCCGGCCTCCAGGGTTTCCTGTGCTGAGT
  GGGGACTTCTTCTCCTACGCTGACCGGGAGGACCACTACTGGACAGGCTATTACACCTCCCGGCCTTTCT
  ACAAGAGCTTGGACCGCGTCCTAGAAGCCCACCTGCGTGGGGCAGAGATTCTATACAGCCTGGCTTTGGC
  CCATGCCCGCCGTTCTGGACTGGCCGGCCAGTACCCGCTGTCTGATTTTGCTCTTCTGACGGAAGCTCGG
  CGTACACTGGGCCTCTTCCAGCACCACGATGCCATCACGGGAACCGCCAAGGAGGCAGTAGTGGTAGACT
  ACGGGGTCAGGTTGCTGCGTTCCCTGGTCAGCCTCAAGCAGGTTATCATCAATGCTGCCCACTACCTGGT
  GCTGGGGGACCAGGAGACCTACAGCTTTGACCCTGGGACACCCTTCCTCCAAATGGATGACAGCCGTGTA
  AGCCATGATGCCCTGCCCGAGCGCACGGTGATCCGGCTGGACTCCTCACCCAGGTTTGTGGTGGTATTTA
  ACCCGCTGGAACAGGAGCGGCTCAGTGTGGTGTCCCTGCTGGTCAACTCGCCCCGGGTGCGCGTCCTTTC
  AGAGGAGGGCCAGCCCCTGTCTGTGCAGATCAGTGTGCACTGGAGCTCGGCTACGGACATGGTCCCTGAT
  GTCTACCAGGTGTCAGTACCCGTCCGCCTGCCAGGCCTGGGCCTGGGTGTGCTGCAGCTGCAGCCAGATC
  TCGATGGGCCCTACACGCTGCAGTCTTCCGTGCGGGTCTACCTGAACGGCGTGAAACTGTCCGTCAGCAG
  GCAGTCAGCATTCCCTGTCCGTGTTGTGGACTCCGGCGCCAGCGACTTCGCCATCAGCAATCGCTACATG
  CAGGTCTGGTTCTCCGGCCTTACTGGGCTTCTCAAGAGCATCCGACGGGTGGATGAGGAGCAGGAGCAGC
  AGATGGAACTGGAGTTCCTCGTCTACGGCACCCGTACCTCCAAGGACAAGAGTGGCGCCTACCTCTTCCT
  GCCTGATAGCGAGGCTAAGCCCTACGTCCCTAAGAAACCTCCTGTGCTGCGTGTCACCGAAGGCCCTTTC
  TTCTCAGAGGTGGCTGTGTACTATGAGCACTTTCACCAAGTGATTCGACTTTATAACCTGCCAGGGGTAG
  AGGGGCTGTCTCTGGACATGTCATTCCAGGTAGATATCAGGGACTATGTGAACAAGGAACTGGCCCTGCG
  CATCCACACAGACATTGACAGCCAGGGCACTTTCTTCACAGACCTCAATGGCTTTCAGATACAGCCCCGG
  CAGTATCTGAAGAAGCTGCCCCTGCAGGCTAATTTCTACCCTATGCCAGTCATGGCCTATATCCAAGATT
  CCCAGAGGCGCCTCACGCTGCACACTGCCCAGGCTCTGGGTGTCTCCAGCCTCGGTAATGGCCAGCTGGA
  GGTGATCTTGGACCGAAGGCTAATGCAGGATGACAACCGGGGACTAGGCCAAGGGCTCAAAGACAACAAG
  ATCACCTGCAACCGTTTCCGCCTCCTGTTAGAACGTCGGACCACAATGAGCCCAGAGGTGCATCAGGAGC
  AGGAGCGTTCTACAAGCTACCCGTCCCTCCTCAGCCACCTGACATCCATGTACCTCAGCACACCTCCTCT
  TGTCTTACCCGTGGCCAAGAGGCAGGGCACTAGCCCTGCTCTGCGCTCTTTCCACCCTCTGGCTTCTCCA
  TTGCCCTGTGACTTCCATTTGCTCAATCTGCGCATGCTCCCAGCCGAGGACACCTTGCCCGCAACTGACT
  CTGCGCTCATCTTACACCGCAAGGGGTTTGACTGTGGCCTTGAAGCCAAGAACCTGGGCTTCAACTGTAC
  CACAAGCCAAGGCAAGCTAGCCCTGGGGAGCCTCTTCCATGGCCTGGATGTGACCTTCCTGCAGCCAACC
  TCCTTGACTTTGCTATACCCTCTGGCCTCACCTTCCAACAGCACTGACATCTCTCTGGAGCCCATGGAGA
  TCAGCACCTTCCGCCTGCGCTTGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215340 representing NM_172903
  Red=Cloning site Green=Tags(s)
  
  MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPARHQNGGNFPRSQISVLQNRIEQLEQLLEENHDIISR
  IKDSVLELTANAEGPPALLPYHTANGSWAVLPEPRPSFFSVSPQDCQFALGGRGQKPELQMLTVSEDLPF
  DNVEGGVWRQGFDISYSPNDWDTEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRR
  FLWAEVSFFAKWWDNISAQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALVDQLIEGHQWLERNLGA
  TPRSGWAVDPFGHSSTMPYLLRRANLTSMLIQRVHYAIKKHFAATHSLEFMWRQMWDSDSSTDIFCHMMP
  FYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVADRAALLLDQYRKKSRLFRSNVLL
  VPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPEFHVQAQFGTLSEYFDALYKRTGVEPGARPPGFPVLS
  GDFFSYADREDHYWTGYYTSRPFYKSLDRVLEAHLRGAEILYSLALAHARRSGLAGQYPLSDFALLTEAR
  RTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVSLKQVIINAAHYLVLGDQETYSFDPGTPFLQMDDSRV
  SHDALPERTVIRLDSSPRFVVVFNPLEQERLSVVSLLVNSPRVRVLSEEGQPLSVQISVHWSSATDMVPD
  VYQVSVPVRLPGLGLGVLQLQPDLDGPYTLQSSVRVYLNGVKLSVSRQSAFPVRVVDSGASDFAISNRYM
  QVWFSGLTGLLKSIRRVDEEQEQQMELEFLVYGTRTSKDKSGAYLFLPDSEAKPYVPKKPPVLRVTEGPF
  FSEVAVYYEHFHQVIRLYNLPGVEGLSLDMSFQVDIRDYVNKELALRIHTDIDSQGTFFTDLNGFQIQPR
  QYLKKLPLQANFYPMPVMAYIQDSQRRLTLHTAQALGVSSLGNGQLEVILDRRLMQDDNRGLGQGLKDNK
  ITCNRFRLLLERRTTMSPEVHQEQERSTSYPSLLSHLTSMYLSTPPLVLPVAKRQGTSPALRSFHPLASP
  LPCDFHLLNLRMLPAEDTLPATDSALILHRKGFDCGLEAKNLGFNCTTSQGKLALGSLFHGLDVTFLQPT
  SLTLLYPLASPSNSTDISLEPMEISTFRLRLG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172903
• ORF Size: 3456 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172903.4, NP_766491.2
• RefSeq Size: 4022 bp
• RefSeq ORF: 3459 bp
• Locus ID: 140481
• UniProt ID: Q8BRK9
• Cytogenetics: 7 D2
• MW: 131.1 kDa
• Gene Summary: Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway (By similarity).[UniProtKB/Swiss-Prot Function]