Man2a2 (NM_172903) Mouse Untagged Clone

CAT#: MC221887

Man2a2 (untagged) - Mouse mannosidase 2, alpha 2 (Man2a2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Man2a2
• Synonyms: 1700052O22Rik; 4931438M07Rik; AI480988; Man IIx; MX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221887 representing NM_172903
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCTGAAAAAGCAGGTGACGGTGTGTGGGGCTGCTATCTTCTGTGTGGCCGTCTTTTCTCTGTACC
  TTATGCTGGACCGAGTGCAGCATGATCCTGCCAGACACCAGAATGGTGGGAACTTCCCCAGGAGCCAAAT
  CTCTGTGCTACAGAACCGGATAGAACAGCTGGAACAACTGCTGGAGGAAAACCATGACATCATAAGCCGT
  ATCAAGGACTCTGTACTGGAACTGACAGCCAATGCGGAGGGCCCGCCAGCCCTGCTGCCCTACCATACCG
  CCAACGGTTCCTGGGCTGTGCTCCCGGAGCCCCGGCCCAGCTTCTTCTCCGTCTCCCCCCAGGACTGCCA
  GTTTGCTTTGGGGGGACGGGGTCAGAAGCCAGAACTACAGATGTTAACCGTGTCTGAGGATTTGCCGTTT
  GACAATGTGGAGGGTGGCGTGTGGAGGCAAGGCTTTGACATCTCCTACAGCCCAAATGACTGGGACACTG
  AAGACCTGCAAGTGTTTGTGGTGCCTCACTCCCACAACGATCCAGGCTGGATCAAGACTTTTGACAAGTA
  CTACACGGAACAGACCCAGCACATCCTCAACAGCATGGTGTCCAAGCTGCAGGAAGACCCCCGGCGGCGC
  TTTCTCTGGGCAGAAGTCTCCTTCTTCGCCAAGTGGTGGGACAACATCAGTGCCCAAAAGAGGGCAGCAG
  TTCGAAGGCTGGTGGGAAATGGGCAGCTGGAGATTGCGACAGGCGGATGGGTGATGCCCGACGAGGCCAA
  CTCCCATTACTTTGCCTTGGTTGACCAGCTCATCGAGGGGCACCAGTGGCTGGAGAGAAACCTGGGTGCA
  ACCCCGCGCTCGGGCTGGGCAGTGGACCCCTTTGGACACAGCTCCACCATGCCTTACCTGCTGCGCCGTG
  CCAACCTGACCAGCATGCTGATTCAGAGGGTGCATTATGCCATCAAGAAGCACTTTGCTGCCACTCATAG
  CCTGGAGTTCATGTGGAGGCAGATGTGGGATTCAGACTCCAGCACAGACATCTTTTGCCACATGATGCCC
  TTCTACAGCTACGACGTTCCACACACCTGTGGCCCCGATCCCAAGATCTGCTGCCAGTTTGATTTCAAAC
  GTCTGCCGGGTGGGAGAATCAATTGCCCTTGGAAGGTGCCACCCCGGGCTATTACAGAAGCCAATGTGGC
  AGACAGGGCAGCCCTGCTCCTGGACCAGTACCGGAAGAAGTCCCGGCTGTTCCGAAGCAACGTCCTCCTC
  GTGCCACTTGGTGATGACTTCCGGTATGACAAGCCCCAGGAATGGGATGCCCAGTTCTTCAACTACCAGC
  GGCTCTTTGACTTCCTCAACAGCAAGCCTGAGTTCCATGTGCAGGCCCAGTTTGGGACCCTCTCTGAGTA
  TTTCGATGCCCTGTATAAGAGGACAGGAGTAGAGCCTGGTGCCCGGCCTCCAGGGTTTCCTGTGCTGAGT
  GGGGACTTCTTCTCCTACGCTGACCGGGAGGACCACTACTGGACAGGCTATTACACCTCCCGGCCTTTCT
  ACAAGAGCTTGGACCGCGTCCTAGAAGCCCACCTGCGTGGGGCAGAGATTCTATACAGCCTGGCTTTGGC
  CCATGCCCGCCGTTCTGGACTGGCCGGCCAGTACCCGCTGTCTGATTTTGCTCTTCTGACGGAAGCTCGG
  CGTACACTGGGCCTCTTCCAGCACCACGATGCCATCACGGGAACCGCCAAGGAGGCAGTAGTGGTAGACT
  ACGGGGTCAGGTTGCTGCGTTCCCTGGTCAGCCTCAAGCAGGTTATCATCAATGCTGCCCACTACCTGGT
  GCTGGGGGACCAGGAGACCTACAGCTTTGACCCTGGGACACCCTTCCTCCAAATGGATGACAGCCGTGTA
  AGCCATGATGCCCTGCCCGAGCGCACGGTGATCCGGCTGGACTCCTCACCCAGGTTTGTGGTGGTATTTA
  ACCCGCTGGAACAGGAGCGGCTCAGTGTGGTGTCCCTGCTGGTCAACTCGCCCCGGGTGCGCGTCCTTTC
  AGAGGAGGGCCAGCCCCTGTCTGTGCAGATCAGTGTGCACTGGAGCTCGGCTACGGACATGGTCCCTGAT
  GTCTACCAGGTGTCAGTACCCGTCCGCCTGCCAGGCCTGGGCCTGGGTGTGCTGCAGCTGCAGCCAGATC
  TCGATGGGCCCTACACGCTGCAGTCTTCCGTGCGGGTCTACCTGAACGGCGTGAAACTGTCCGTCAGCAG
  GCAGTCAGCATTCCCTGTCCGTGTTGTGGACTCCGGCGCCAGCGACTTCGCCATCAGCAATCGCTACATG
  CAGGTCTGGTTCTCCGGCCTTACTGGGCTTCTCAAGAGCATCCGACGGGTGGATGAGGAGCAGGAGCAGC
  AGATGGAACTGGAGTTCCTCGTCTACGGCACCCGTACCTCCAAGGACAAGAGTGGCGCCTACCTCTTCCT
  GCCTGATAGCGAGGCTAAGCCCTACGTCCCTAAGAAACCTCCTGTGCTGCGTGTCACCGAAGGCCCTTTC
  TTCTCAGAGGTGGCTGTGTACTATGAGCACTTTCACCAAGTGATTCGACTTTATAACCTGCCAGGGGTAG
  AGGGGCTGTCTCTGGACATGTCATTCCAGGTAGATATCAGGGACTATGTGAACAAGGAACTGGCCCTGCG
  CATCCACACAGACATTGACAGCCAGGGCACTTTCTTCACAGACCTCAATGGCTTTCAGATACAGCCCCGG
  CAGTATCTGAAGAAGCTGCCCCTGCAGGCTAATTTCTACCCTATGCCAGTCATGGCCTATATCCAAGATT
  CCCAGAGGCGCCTCACGCTGCACACTGCCCAGGCTCTGGGTGTCTCCAGCCTCGGTAATGGCCAGCTGGA
  GGTGATCTTGGACCGAAGGCTAATGCAGGATGACAACCGGGGACTAGGCCAAGGGCTCAAAGACAACAAG
  ATCACCTGCAACCGTTTCCGCCTCCTGTTAGAACGTCGGACCACAATGAGCCCAGAGGTGCATCAGGAGC
  AGGAGCGTTCTACAAGCTACCCGTCCCTCCTCAGCCACCTGACATCCATGTACCTCAGCACACCTCCTCT
  TGTCTTACCCGTGGCCAAGAGGCAGGGCACTAGCCCTGCTCTGCGCTCTTTCCACCCTCTGGCTTCTCCA
  TTGCCCTGTGACTTCCATTTGCTCAATCTGCGCATGCTCCCAGCCGAGGACACCTTGCCCGCAACTGACT
  CTGCGCTCATCTTACACCGCAAGGGGTTTGACTGTGGCCTTGAAGCCAAGAACCTGGGCTTCAACTGTAC
  CACAAGCCAAGGCAAGCTAGCCCTGGGGAGCCTCTTCCATGGCCTGGATGTGACCTTCCTGCAGCCAACC
  TCCTTGACTTTGCTATACCCTCTGGCCTCACCTTCCAACAGCACTGACATCTCTCTGGAGCCCATGGAGA
  TCAGCACCTTCCGCCTGCGCTTGGGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172903
• Insert Size: 3459 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172903.4, NP_766491.2
• RefSeq Size: 6575 bp
• RefSeq ORF: 3459 bp
• Locus ID: 140481
• UniProt ID: Q8BRK9
• Cytogenetics: 7 D2
• Gene Summary: Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway (By similarity).[UniProtKB/Swiss-Prot Function]