Arhgef5 (NM_133674) Mouse Tagged ORF Clone
CAT#: MR215297
- TrueORF®
Arhgef5 (Myc-DDK-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 5 (Arhgef5)
"NM_133674" in other vectors (6)
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Product Images

Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Arhgef5 |
Synonyms | 2210412D05Rik; AA717842; AW495314; Tim1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR215297 representing NM_133674
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCTGAGGAGCCTGAATATGGAGTATCTACAGAAGTCCCTGACATAGAAGAATTGAAAACTATCC CTGAAGGTATCATGAGGAGCAGTCAGATCCCTGCCTTGGATCCTGAAGCTCAAGAAGACCGAGACCCATC CTATAAGTGGACAGATGGACACAGACCCGTGATGAACCAGTCAAAAGTGTTAAGGGACATGGGTGACCAT ACACCTAATAGTATGGCAATTTTCTTCAAGAAAGAATCTTCGGATATGGAGACAAGCCAGGAAATCCTTC TGGCTGAGGCCTGTAACACTCCAGACCAGCAGGAAGCTGTAATCCAGAGCCTTAAAGACAGACTGTCAAG AACAATAGCTGCCCCTGAGCTCTTGGCATGTGCTGTCCAGGAAGAATGGCTAGACATACCCAGCAAACTA GACAACAGAGTGGGGGCAGAATTGCAGTCAGAACTTATGTCTTTGACTTTGGCAGTTAGCAAAGAGAAAG AAGAGGAAGAAACCTCCCCAGACACCTCAATCCCTAGAGGATCTTGGCCTCCCTGCAAAACTCACCCTGG TGAGACTGAGCAGACCCAGGGCAGTGGAAGTGAACTGCTTAGGCAGGGGAAACAGCTGCAGTTGGAGGCC ACACAAGAAAATCAAGGGCAAGAAGGCTTCCTTCAATCTCAGGAAGCCCAGGGACTGGAGGAGCAGGAGG GACAGGAAGTAGAAATTCAGGAGGAAGGAACTCTGAACGAAGGCATTTGTTTTGGTGGGCTCCTGGGAGA GCAGGAGGAGGTGGAAGAGGGGTTTAACGGCAACGAGGAAGAACAGAAGCAGGGACAGATCCAAAGCTAT ATGCTACTTGGAGGACAATGGGAAAATGAGGGGCTCAGTGGGGAATTAGAAGGTCTGAATTACAGTGAGA GAGGCCAAGAGAACAGGGAAAGGAGGGTTTGGGTTCTGAGAGATTCAGAAGAGGAGGGACAGGACCAGGA ATCCAGGGAGGTGGAAGAGAGAAGGGTAGCCACTCAGTATACAGAGAATCAAAGGTTAGTAGAGAAATCA GAGATTGTAAAGAGAAAGCAGAGAGATCATGATCAAACAGGAAAAGTGATGCCCGTAAGGGACCAGAAGG AGGTTGTAGACTCAGGTGACAGGGTCCAAGGGAATGGGGATAGTGGTGGGCAGACAGCAGTGGAAGGGAG CAGACCAGGAGAGGACAGCAAGCCTTCTCTTCCAGTGGCTTCCGTAGATCCTGAGGTTCTCTCCCCAGGT ACATTGTTCCCAGGTATTTCTTCCTCTGTGGCTGATATTCCACAGATTCAGAAGGAGCCTGTGTGTGAGG AACTGAGCCCTCAAGCTCCTGCACTGGAGCCAACAGAATGGTCTCACCAACCCATTTCTCCCCCTGCCTC TTTTGCTCCTGAAGAATCTCTTGATAATAGGACTCACAACAGCCAGCAAGAAGAATTCAGGCTAAGGAAG GGGATTGAGGTTGTCTCTGCTAGTACATCTGTGGCTCCTTCAGGGACACGAGATTCACCTCCTTTCAGTC CTCCTAATGTTTTCTCCAGCACTGCCACCTTGTCACCTGTTAGCTCTTCAGTTATCCTTCCTGAGGAGAC CCCAACAGCCTCTGCCTCAGCTGACACTCCACATCATTGTGGGCCATGTGAGACTCCTCCACTACCCGCA AAATCCTCCAGGTACCCATGTGCCACCTCTGACACAGCCAACCCTCACAGCCCTCTTAGCAGCTACACTG GAGTTACCCAACATCTAAGAAGCAACTCGTTCCCAGGCTCTCACAGGACAGAGCAGACTCCAGACTCTCT GGGAATGTCACTCTCTTTCTCTCATTTGGAATTACCTCAGAGGCCTCCTAAACCAGCCATCTATGGCTCT CTGACCCCAAGAAGAAACAGAAGAAGTAGGGATGGTATCGTCTTTTCAGACTCCTCTACTGCTTTGTTTG CTTTGAAACAGGACTCTGAAGAATTCACTTCAAATCCAGAGAGGCCCAGCAGTCCTCATGGTAGTCCGAC ATGGGGCTCCCCACAGAACTCAGCCTTTGCCATAGGCTCTCCTGCAAATGTTTCTTCACCACCCACTGTC TCCATGGATATGACGATACGTGAAGCTTTACTCCCTATTCCCCCAGAGAAGAGACATAGCTACAGTCACA TAGTGGAGAGGGATGGCCTTCTTCATGAAGTAGCCTCTACACTGAAGCGGCATAGTCATCCTCCTCCACT GACCCTAAGTTCAGGGCTCCATAGATCTTCTAAAGGCTCATTTTCCCTAGTTCCTGACTCCACTGTGGCA AGGCAGCACCGCCCTCTGCCGTCTACCCCAGAAAGCCCCAACCATACACAGACCTCCATCCCTTCCAGAC TGAGATACAACAAACCATTACCCCCAACTCCTGATATGCCTGAGTTCTACCATCCCTCCATCTCTTCCTC TTATATCTCAAGGATGTATAGGCCTCTACCCCCAGTCCCTATCATAGATCCTTCCTCTGAACCACCTCCA TTGCCCCCAAAGTCCAGGGGGAGAAGCAAGAGTATCCAGGGAGGAGTTATACATTCAGGAGGTCAAGCCA AGCCAAGACCTAATAATCAAGACTGGACAGCTTCTACTCTCTCTGTTGGACGAACCTCCTGGCCTCCAGC CACAGGCAGATCAACAGAATCTTTGCCTCTCACCAGTAGGTGTAATAATGAAGTGTCCCCTGGCCTGGCT TTCAGCAACATGACAAACCTTCTTAGTCCCTCTTCTCCTACTACTCCTTGGATCCCAGACCTTCAGAGAC CTACCACTAAGGATGAGTCAGGGCTCACTGAAGAGTCTGAGCCACCAGTGAGAGGATCATTTAGACGATC TGCCCCTCAGGAGGAATTTAATAATACAAGGAGGTCAGCTTTAGGATCAAGAAAGAACTCAGAAAAACCC CTCCACCACCAACTGGAGAAGGCATCCAGCTGGCCTCACAGACGCGACCCAGCAAGGACATCAGAAAGTA GCAGTGAACAGGTTGTTCTGGGCCAGGTACCCAACAAGCAAAAGGGCTGGAACCGGCAAGGCCTTCGTAG ACCTTCAATTTTGCCTGAAAGCTCTTCAGATTTACGAAATCCAGCTGCGGGAAGACTTCCTGGCTCTTCA GATTCTGTTGTTTTCCGGGAGAAGAAACCAAAGGAGGGGATGGGAGGCTTTTCAAGACGTTGCTCCAAGC TCATCTCCTCCCAGCTACTTTATCAGGAGTACAGTGATGTTGTTCTGAACAAGGAGATTCAGAGCCAGCA GCGGCTGGACAGCCTGGCCGAGCCACATGGGCTCTCTTCCCCACGGCATCGTCGAAAGGCACTGGTCTCT TCAGACTCCTACCTACAGCGCCTCTCTATGGCCTCCAGTGGCTCCCTCTGGCAGGAAATCCCTGTGGTGC GCAATAGCACAGTGCTGCTCTCCATGACCCATGAAGATCAAAAACTGCAAGAGGCCAAGTTTGAGCTGAT TGTGTCAGAAGCATCTTACCTGCGCAGTCTAAACATAGCTGTGGATCATTTCCAACATTCAGCCCAGCTG CGGGGCACCCTGTCCAACCAGGATCATCAGTGGCTCTTCTCTCGTTTGCAGGATGTACGAGATGTCAGCA CCACGTTCCTTTCAGACCTAGAAGAAAACTTTGAGAACAACATCTTCTCCTTCCAAGTGTGTGATGTTGT CTTGAATCATGCCGCAGACTTCCACCGTGTCTACCTGCCTTATGTCACCAACCAGACCTATCAGGAACGC ACCTTCCAAAGTCTAATGAATAGCAACAGCAGTTTCCGGGAGGTCTTGGAGAAGCTAGAGAGCGACCCCA TCTGCCAACGCCTGTCTCTCAAGTCCTTTCTGATACTGCCCTTCCAACGCATCACACGTCTCAAGCTGCT GCTCCAGAACATACTGAAGAGAACTCAGCCTGGCTCCTCAGAAGAGGCAGAGGCCACGAAGGCACACCAT GCCCTAGAGAAGCTGATTCGAGACTGCAACAGCAATGTGCAGAGAATGCGGCGGACAGAGGAGCTCATCT ACCTGAGCCAGAAGATTGAGTTTGAGTGCAAAATTTTCCCACTTATTTCACAATCTCGATGGCTGGTGAA GAGTGGGGAGCTGACGGCCCTTGAGTTCAGTGTCTCCCCAGGGCTGAGAAGGAAACTGACCACTCGTCCA GTCCACCTACATCTCTTCAATGACTGTCTGTTGCTGTCTCGGCCCCGAGAGGGCAGCCGGTTCCTGGTAT TTGACCATGCTCCGTTCTCCTCCATCCGAGGGGAGAAGTGTGAAATGAAGCTTCATGGACCTCACAAAAA CCTCTTCCGTCTCTTTCTCCTGCACAACGCACAGGGCACCCAAGTGGAATTCCTCTTCCGCACTGAGACT CAAAGTGAAAAGCTTCGCTGGATCTCAGCCTTGGCCATGCCCAGAGAGGAGTTGGACCTCCTTGAGTGTT ATGACTCCCCACAAGTTCAGTGCCTCCGTGCCTACAAGCCTCGAGAGAATGATGAGTTGGCACTAGAGAA GGCGGATGTGGTGATGGTCACTCAGCAAAGCAGTGATGGCTGGCTGGAGGGTGTAAGACTCTCAGATGGT GAGCAAGGCTGGTTTCCTGTCCAGCAGGTAGAGTTCATTTCAAACCCAGAGGTCCGTGCTCAGAACCTCA AGGAAGCCCATCGAGTCAAGACGGCCAAACTGCAGCTGGTAGAACAGCAAGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR215297 representing NM_133674
Red=Cloning site Green=Tags(s) MEAEEPEYGVSTEVPDIEELKTIPEGIMRSSQIPALDPEAQEDRDPSYKWTDGHRPVMNQSKVLRDMGDH TPNSMAIFFKKESSDMETSQEILLAEACNTPDQQEAVIQSLKDRLSRTIAAPELLACAVQEEWLDIPSKL DNRVGAELQSELMSLTLAVSKEKEEEETSPDTSIPRGSWPPCKTHPGETEQTQGSGSELLRQGKQLQLEA TQENQGQEGFLQSQEAQGLEEQEGQEVEIQEEGTLNEGICFGGLLGEQEEVEEGFNGNEEEQKQGQIQSY MLLGGQWENEGLSGELEGLNYSERGQENRERRVWVLRDSEEEGQDQESREVEERRVATQYTENQRLVEKS EIVKRKQRDHDQTGKVMPVRDQKEVVDSGDRVQGNGDSGGQTAVEGSRPGEDSKPSLPVASVDPEVLSPG TLFPGISSSVADIPQIQKEPVCEELSPQAPALEPTEWSHQPISPPASFAPEESLDNRTHNSQQEEFRLRK GIEVVSASTSVAPSGTRDSPPFSPPNVFSSTATLSPVSSSVILPEETPTASASADTPHHCGPCETPPLPA KSSRYPCATSDTANPHSPLSSYTGVTQHLRSNSFPGSHRTEQTPDSLGMSLSFSHLELPQRPPKPAIYGS LTPRRNRRSRDGIVFSDSSTALFALKQDSEEFTSNPERPSSPHGSPTWGSPQNSAFAIGSPANVSSPPTV SMDMTIREALLPIPPEKRHSYSHIVERDGLLHEVASTLKRHSHPPPLTLSSGLHRSSKGSFSLVPDSTVA RQHRPLPSTPESPNHTQTSIPSRLRYNKPLPPTPDMPEFYHPSISSSYISRMYRPLPPVPIIDPSSEPPP LPPKSRGRSKSIQGGVIHSGGQAKPRPNNQDWTASTLSVGRTSWPPATGRSTESLPLTSRCNNEVSPGLA FSNMTNLLSPSSPTTPWIPDLQRPTTKDESGLTEESEPPVRGSFRRSAPQEEFNNTRRSALGSRKNSEKP LHHQLEKASSWPHRRDPARTSESSSEQVVLGQVPNKQKGWNRQGLRRPSILPESSSDLRNPAAGRLPGSS DSVVFREKKPKEGMGGFSRRCSKLISSQLLYQEYSDVVLNKEIQSQQRLDSLAEPHGLSSPRHRRKALVS SDSYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEAKFELIVSEASYLRSLNIAVDHFQHSAQL RGTLSNQDHQWLFSRLQDVRDVSTTFLSDLEENFENNIFSFQVCDVVLNHAADFHRVYLPYVTNQTYQER TFQSLMNSNSSFREVLEKLESDPICQRLSLKSFLILPFQRITRLKLLLQNILKRTQPGSSEEAEATKAHH ALEKLIRDCNSNVQRMRRTEELIYLSQKIEFECKIFPLISQSRWLVKSGELTALEFSVSPGLRRKLTTRP VHLHLFNDCLLLSRPREGSRFLVFDHAPFSSIRGEKCEMKLHGPHKNLFRLFLLHNAQGTQVEFLFRTET QSEKLRWISALAMPREELDLLECYDSPQVQCLRAYKPRENDELALEKADVVMVTQQSSDGWLEGVRLSDG EQGWFPVQQVEFISNPEVRAQNLKEAHRVKTAKLQLVEQQV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_133674 |
ORF Size | 4743 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_133674.1, NP_598435.1 |
RefSeq Size | 5420 bp |
RefSeq ORF | 4746 bp |
Locus ID | 54324 |
UniProt ID | E9Q7D5 |
Cytogenetics | 6 B2.1 |
MW | 176.7 kDa |
Gene Summary | Guanine nucleotide exchange factor which activates Rho GTPases (PubMed:19713215, PubMed:21525037). Strongly activates RHOA (PubMed:19713215, PubMed:21525037). Also strongly activates RHOB, weakly activates RHOC and RHOG and shows no effect on RHOD, RHOV, RHOQ or RAC1 (PubMed:19713215). Involved in regulation of cell shape and actin cytoskeletal organization (PubMed:21525037). Plays a role in actin organization by generating a loss of actin stress fibers and the formation of membrane ruffles and filopodia (By similarity). Required for SRC-induced podosome formation (PubMed:21525037). Involved in positive regulation of immature dendritic cell migration (PubMed:19713215).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC224710 | Arhgef5 (untagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 5 (Arhgef5), (10ug) |
USD 1,488.00 |
|
MG215297 | Arhgef5 (tGFP-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 5 (Arhgef5), (10ug) |
USD 2,363.00 |
|
MR215297L1 | Lenti ORF clone of Arhgef5 (Myc-DDK-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 5 (Arhgef5) |
USD 2,463.00 |
|
MR215297L2 | Lenti ORF clone of Arhgef5 (mGFP-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 5 (Arhgef5) |
USD 2,463.00 |
|
MR215297L3 | Lenti ORF clone of Arhgef5 (Myc-DDK-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 5 (Arhgef5) |
USD 2,463.00 |
|
MR215297L4 | Lenti ORF clone of Arhgef5 (mGFP-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 5 (Arhgef5) |
USD 2,463.00 |
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