Slit1 (NM_015748) Mouse Tagged ORF Clone

CAT#: MR212022

Slit1 (Myc-DDK-tagged) - Mouse slit homolog 1 (Drosophila) (Slit1)

Product Images

Circular map for MR212022

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slit1
• Synonyms: mKIAA0813; Slil1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR212022 representing NM_015748
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTAACGCCCCAGCGCGGGTCCTCTTCGGGGTTGAGCCGACCGGAGCTGTGGCTGCTGCTGTGGG
  CAGCCGCGTGGCGCCTGGGGGCCACGGCGTGCCCCGCCCTCTGCACCTGCACCGGCACTACGGTGGACTG
  CCACGGCACGGGTTTGCAGGCCATCCCCAAGAACATCCCACGGAACACCGAGCGCCTGGAACTCAACGGC
  AACAACATCACTCGGATCCACAAGAATGACTTTGCTGGCCTCAAGCAGCTTCGAGTGTTGCAGCTGATGG
  AGAACCAGATCGGAGCTGTGGAACGCGGAGCTTTTGATGACATGAAGGAGCTAGAGCGCCTACGGCTGAA
  CCGCAACCAGCTACAAGTGTTGCCTGAACTACTGTTCCAGAACAACCAGGCTCTATCCAGACTGGACCTG
  AGTGAGAATTTTCTCCAGGCTGTGCCCAGGAAGGCTTTTCGGGGAGCCACAGACCTCAAAAATCTACAGC
  TGGACAAGAACCGGATCAGCTGCATCGAGGAAGGGGCCTTCCGTGCCCTACGGGGACTGGAGGTTCTGAC
  CCTGAACAACAACAACATCACCACCATCCCTGTGTCCAGTTTCAACCATATGCCCAAGCTTCGGACCTTC
  CGGCTGCACTCCAACCACCTGTTCTGTGACTGCCACCTGGCCTGGCTCTCTCAGTGGCTGAGACAGAGGC
  CCACCATCGGACTCTTCACCCAGTGCTCTGGGCCCGCCAGCCTTCGGGGCCTCAACGTGGCAGAGGTGCA
  AAAGGGCGAGTTCAGCTGCTCAGGCCAGGGGGAGGCTGCGGGAGCACCTGCCTGCACCCTCTCGTCCGGT
  TCCTGCCCAGCCATGTGCAGCTGTAGCAGCGGCATCGTGGACTGCCGTGGCAAGGGCCTCACTGCCATCC
  CTGCTAACCTTCCAGAGACCATGACAGAGATCCGCCTGGAGTTGAACGGTATCAAATCCATCCCACCCGG
  AGCCTTCTCTCCCTACAGAAAGCTGCGCAGGATAGACCTGAGCAACAACCAGATTGCAGAGATTGCACCC
  GACGCCTTCCAAGGCCTGCGCTCCTTGAATTCCCTGGTCCTCTATGGCAACAAGATTACAGACCTGCCCC
  GAGGTGTATTTGGAGGCCTGTACACACTCCAGCTGCTGCTTCTAAATGCCAACAAGATCAACTGCATCCG
  GCCTGATGCCTTCCAGGACCTGCAGAACCTCTCTCTGCTCTCCCTGTATGACAACAAGATCCAGAGTCTC
  GCCAAGGGCACCTTCACCTCCCTACGGGCCATCCAGACACTGCATCTGGCCCAGAATCCTTTCATCTGTG
  ACTGTAACCTCAAGTGGCTGGCAGACTTCTTACGTACCAACCCCATCGAGACAACTGGGGCCCGATGTGC
  CAGCCCCCGGCGCCTCGCCAACAAGCGTATTGGGCAGATCAAAAGCAAGAAGTTTCGGTGCTCAGCCAAA
  GAGCAGTACTTCATTCCAGGGACAGAGGACTACCACCTGAACAGCGAGTGTACCAGCGACGTGGCCTGCC
  CACACAAGTGCCGTTGTGAGGCCAGTGTGGTGGAGTGCTCCAGCCTGAAACTCTCTAAGATCCCTGAGCG
  AATCCCCCAGTCCACGACTGAGCTACGACTGAACAACAATGAGATTTCTATCCTAGAGGCCACTGGGTTG
  TTTAAGAAGCTCTCACATCTGAAGAAAATAAACCTGAGCAATAACAAGGTGTCCGAGATCGAAGACGGGA
  CGTTTGAGGGTGCCGCCTCTGTCAGTGAGCTGCACCTGACTGCTAACCAGCTGGAGTCCATTAGAAGTGG
  CATGTTCCGGGGTCTAGATGGCTTGCGGACCCTGATGCTGAGGAACAACCGTATCAGCTGTATCCACAAC
  GACAGCTTCACCGGGCTGCGCAACGTGCGGCTCCTGTCTCTCTATGACAACCACATCACCACCATCTCCC
  CTGGAGCCTTTGACACTCTCCAGGCCCTGTCCACACTAAACCTCCTGGCCAACCCGTTCAACTGCAACTG
  CCATCTGTCATGGCTGGGCGACTGGCTCCGGAAGAGGAAGATTGTGACGGGGAACCCTCGCTGTCAGAAC
  CCAGACTTCCTGCGGCAGATTCCTCTGCAGGATGTGGCCTTCCCTGACTTCAGGTGTGAGGAAGGCCAGG
  AGGAGGTGGGCTGCCTGCCCCGCCCACAGTGCCCCCAGGAGTGTGCCTGCCTGGACACAGTGGTCCGCTG
  CAGCAACAAGCACCTCCAGGCTCTGCCCAAGGGCATCCCCAAGAATGTCACAGAACTCTATTTGGATGGG
  AACCAGTTCACACTCGTTCCGGGACAGCTGTCTACCTTCAAGTATCTGCAGCTCGTGGACTTGAGCAACA
  ACAAGATCAGTTCCTTGAGCAATTCTTCCTTCACCAACATGAGCCAGCTGACCACGCTGATCCTCAGCTA
  CAATGCCCTCCAGTGCATCCCTCCTCTGGCCTTTCAAGGACTTCGTTCGCTGCGCCTGCTGTCTTTGCAT
  GGCAATGATGTCTCCACCCTCCAAGAGGGCATCTTTGCAGACGTGACGTCTCTGTCTCACCTGGCCATTG
  GTGCCAACCCCCTGTACTGCGACTGCCGCCTCCGGTGGCTCTCCAGTTGGGTGAAGACTGGCTACAAGGA
  ACCTGGCATTGCCCGCTGTGCTGGACCCCCAGAGATGGAGGGCAAACTGTTACTCACCACACCTGCCAAG
  AAGTTTGAATGCCAAGGTCCCCCCAGCCTGGCAGTTCAGGCCAAGTGTGATCCCTGCTTGTCCAGCCCAT
  GCCAGAACCAGGGCACCTGCCACAATGACCCTCTTGAGGTATACAGGTGTACCTGCCCCAGTGGCTACAA
  GGGCCGACACTGTGAGGTGTCTCTGGACGGCTGTTCCAGCAACCCATGTGGAAATGGGGGGACCTGCCAT
  GCCCAGGAGGGCGAGGATGCTGGCTTCACGTGCTCCTGTCCCTCCGGCTTTGAAGGGCCGACCTGTGGAG
  TGGACACAGATGACTGTGTTAAGCATGCCTGTGTCAACGGGGGTGTCTGTGTAGATGGCGTGGGGAATTA
  CACATGTCAGTGCCCCCTGCAGTACACAGGAAGGGCCTGTGAGCAGCTGGTGGACTTCTGCTCCCCGGAT
  ATGAACCCGTGTCAGCATGAGGCCCAATGTGTTGGCACCCCGGATGGGCCCAGGTGTGAGTGCATGCTAG
  GTTACACGGGTGACAACTGCAGCGAAAATCAGGATGACTGCAAGGACCACAAGTGTCAGAACGGGGCCCA
  GTGTGTGGATGAAGTCAACAGCTACGCCTGCCTCTGTGTTGAGGGCTACAGTGGACAGCTCTGTGAGATC
  CCACCTGCCCCCAGGAGCTCCTGTGAGGGGACGGAGTGCCAGAATGGAGCCAACTGCGTAGACCAGGGAA
  GCCGGCCCGTATGCCAGTGCCTACCAGGCTTTGGTGGCCCTGAATGTGAGAAATTGCTCAGTGTCAACTT
  TGTGGACCGGGACACTTATCTGCAGTTCACTGACCTGCAGAACTGGCCTCGGGCCAACATCACTCTTCAG
  GTCTCCACAGCAGAGGACAATGGGATTCTCCTATATAATGGGGACAATGACCACATTGCAGTTGAGCTGT
  ACCAGGGCCATGTCCGTGTTAGTTATGACCCAGGCAGCTACCCCAGCTCTGCTATCTACAGCGCTGAGAC
  GATCAACGATGGGCAGTTCCACACCGTTGAGCTGGTCACCTTCGACCAGATGGTGAACCTCTCCATCGAT
  GGCGGCAGCCCCATGACCATGGACAACTTTGGCAAGCACTACACCCTCAACAGCGAGGCCCCCCTCTATG
  TGGGAGGGATGCCTGTGGACGTGAACTCAGCTGCCTTCCGCCTGTGGCAGATCCTCAATGGTACCAGCTT
  CCACGGTTGCATCCGGAACCTGTACATCAACAATGAACTGCAGGACTTCACCAAGACACAGATGAAGCCA
  GGCGTGGTGCCAGGCTGTGAGCCGTGCCGCAAACTCTACTGTCTACATGGCATTTGCCAGCCCAACGCCA
  CCCCAGGGCCTGTGTGCCACTGTGAGGCTGGCTGGGGGGGCCTGCACTGTGACCAGCCAGTGGATGGCCC
  CTGCCATGGCCACAAGTGTGTCCATGGGAAATGCGTGCCGCTCGACGCTCTTGCCTACAGCTGCCAGTGC
  CAGGATGGGTACTCGGGGGCTCTGTGCAACCAGGTCGGGGCTGTGGCAGAGCCCTGTGGGGGCTTGCAGT
  GCCTGCATGGTCACTGCCAGGCCTCTGCCACCAAAGGGGCACACTGTGTGTGCAGCCCAGGCTTTTCAGG
  CGAGCTGTGTGAGCAAGAGTCCGAGTGCCGGGGGGACCCTGTCCGGGACTTTCACCGGGTCCAGAGGGGC
  TATGCCATCTGCCAGACCACACGCCCGCTGTCGTGGGTGGAATGCCGGGGCGCGTGCCCGGGCCAGGGCT
  GCTGCCAGGGCCTGCGGCTGAAGCGGAGGAAGCTCACCTTCGAGTGCAGCGATGGGACTTCGTTTGCTGA
  GGAGGTGGAGAAGCCCACCAAGTGTGGCTGTGCCCAGTGCGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR212022 representing NM_015748
  Red=Cloning site Green=Tags(s)
  
  MALTPQRGSSSGLSRPELWLLLWAAAWRLGATACPALCTCTGTTVDCHGTGLQAIPKNIPRNTERLELNG
  NNITRIHKNDFAGLKQLRVLQLMENQIGAVERGAFDDMKELERLRLNRNQLQVLPELLFQNNQALSRLDL
  SENFLQAVPRKAFRGATDLKNLQLDKNRISCIEEGAFRALRGLEVLTLNNNNITTIPVSSFNHMPKLRTF
  RLHSNHLFCDCHLAWLSQWLRQRPTIGLFTQCSGPASLRGLNVAEVQKGEFSCSGQGEAAGAPACTLSSG
  SCPAMCSCSSGIVDCRGKGLTAIPANLPETMTEIRLELNGIKSIPPGAFSPYRKLRRIDLSNNQIAEIAP
  DAFQGLRSLNSLVLYGNKITDLPRGVFGGLYTLQLLLLNANKINCIRPDAFQDLQNLSLLSLYDNKIQSL
  AKGTFTSLRAIQTLHLAQNPFICDCNLKWLADFLRTNPIETTGARCASPRRLANKRIGQIKSKKFRCSAK
  EQYFIPGTEDYHLNSECTSDVACPHKCRCEASVVECSSLKLSKIPERIPQSTTELRLNNNEISILEATGL
  FKKLSHLKKINLSNNKVSEIEDGTFEGAASVSELHLTANQLESIRSGMFRGLDGLRTLMLRNNRISCIHN
  DSFTGLRNVRLLSLYDNHITTISPGAFDTLQALSTLNLLANPFNCNCHLSWLGDWLRKRKIVTGNPRCQN
  PDFLRQIPLQDVAFPDFRCEEGQEEVGCLPRPQCPQECACLDTVVRCSNKHLQALPKGIPKNVTELYLDG
  NQFTLVPGQLSTFKYLQLVDLSNNKISSLSNSSFTNMSQLTTLILSYNALQCIPPLAFQGLRSLRLLSLH
  GNDVSTLQEGIFADVTSLSHLAIGANPLYCDCRLRWLSSWVKTGYKEPGIARCAGPPEMEGKLLLTTPAK
  KFECQGPPSLAVQAKCDPCLSSPCQNQGTCHNDPLEVYRCTCPSGYKGRHCEVSLDGCSSNPCGNGGTCH
  AQEGEDAGFTCSCPSGFEGPTCGVDTDDCVKHACVNGGVCVDGVGNYTCQCPLQYTGRACEQLVDFCSPD
  MNPCQHEAQCVGTPDGPRCECMLGYTGDNCSENQDDCKDHKCQNGAQCVDEVNSYACLCVEGYSGQLCEI
  PPAPRSSCEGTECQNGANCVDQGSRPVCQCLPGFGGPECEKLLSVNFVDRDTYLQFTDLQNWPRANITLQ
  VSTAEDNGILLYNGDNDHIAVELYQGHVRVSYDPGSYPSSAIYSAETINDGQFHTVELVTFDQMVNLSID
  GGSPMTMDNFGKHYTLNSEAPLYVGGMPVDVNSAAFRLWQILNGTSFHGCIRNLYINNELQDFTKTQMKP
  GVVPGCEPCRKLYCLHGICQPNATPGPVCHCEAGWGGLHCDQPVDGPCHGHKCVHGKCVPLDALAYSCQC
  QDGYSGALCNQVGAVAEPCGGLQCLHGHCQASATKGAHCVCSPGFSGELCEQESECRGDPVRDFHRVQRG
  YAICQTTRPLSWVECRGACPGQGCCQGLRLKRRKLTFECSDGTSFAEEVEKPTKCGCAQCA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015748
• ORF Size: 4593 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015748.3, NP_056563.2
• RefSeq Size: 5300 bp
• RefSeq ORF: 4596 bp
• Locus ID: 20562
• UniProt ID: Q80TR4
• Cytogenetics: 19 C3
• MW: 167.9 kDa
• Gene Summary: Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.[UniProtKB/Swiss-Prot Function]