Mgat5b (NM_172948) Mouse Tagged Lenti ORF Clone
CAT#: MR210677L4
- LentiORF®
Lenti ORF clone of Mgat5b (mGFP-tagged) - Mouse mannoside acetylglucosaminyltransferase 5, isoenzyme B (Mgat5b)
"NM_172948" in other vectors (4)
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USD 365.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | mGFP |
Symbol | Mgat5b |
Synonyms | C330018B01; GnT-IX; mGnTVB |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR210677).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_172948 |
ORF Size | 2376 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_172948.3, NP_766536.2 |
RefSeq Size | 4277 bp |
RefSeq ORF | 2379 bp |
Locus ID | 268510 |
UniProt ID | Q765H6 |
Cytogenetics | 11 E2 |
Gene Summary | Glycosyltransferase that acts on alpha-linked mannose of N-glycans and O-mannosyl glycans. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to the beta 1-6 linkage of the mannose residue of GlcNAc-beta1,2-Man-alpha on both the alpha1,3- and alpha1,6-linked mannose arms in the core structure of N-glycan (By similarity). Also acts on the GlcNAc-beta1,2-Man-alpha1-Ser/Thr moiety, forming a 2,6-branched structure in brain O-mannosyl glycan (PubMed:22715095). Plays an active role in modulating integrin and laminin-dependent adhesion and migration of neuronal cells via its activity in the O-mannosyl glycan pathway.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC221615 | Mgat5b (untagged) - Mouse mannoside acetylglucosaminyltransferase 5, isoenzyme B (Mgat5b), (10ug) |
USD 799.00 |
|
MG210677 | Mgat5b (tGFP-tagged) - Mouse mannoside acetylglucosaminyltransferase 5, isoenzyme B (Mgat5b) |
USD 925.00 |
|
MR210677 | Mgat5b (Myc-DDK-tagged) - Mouse mannoside acetylglucosaminyltransferase 5, isoenzyme B (Mgat5b) |
USD 725.00 |
|
MR210677L3 | Lenti ORF clone of Mgat5b (Myc-DDK-tagged) - Mouse mannoside acetylglucosaminyltransferase 5, isoenzyme B (Mgat5b) |
USD 1,025.00 |
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