Mgat5b (NM_172948) Mouse Untagged Clone

CAT#: MC221615

Mgat5b (untagged) - Mouse mannoside acetylglucosaminyltransferase 5, isoenzyme B (Mgat5b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mgat5b
• Synonyms: C330018B01; GnT-IX; mGnTVB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221615 representing NM_172948
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCACAGTCAACCCAGATGGGAAGATAATGGTCAGAAGATGCCTGGTCACCCTGAGACCCTTTCGGC
  TGTTTGTCCTGGGCATCGGCTTCTTCACTCTCTGCTTCCTGATGACATCTTTGGGAGGCCAGTTCTCTGC
  CCGGCGCCTGGGGGACTCGCCCTTCACCATCCGCACAGAAGTGCCAGGCAGCCCAGAGTCACGTGGTGCC
  CTTCGCAAGATGAGCGACCTGCTGGAGCTGATGGTGAAGCGCATGGATATGCTGGCCAGGCTGGAGAATA
  GCAGCGAGCTGCACCGGACTGCCAGTGTGGCGCACTTAGCCGCAGACAGGCTCACCCCTGGGGCCAGCCT
  CATTGAAAGGATCCAGGCCATTGCCCAGAATGTGTCTGACATCGCTGTGAAGGTGGACCAGATCCTGCGC
  CACAGCCTGATTCTGCATAGCAAGGTGTCTGAAGGTCGGAGGGACCAGTGTGAAGCACCCAGTGACCCCA
  AGTTCCCTGACTGTTCCGGGAAAGTGGAGTGGATGCGCGCCCGCTGGACCTCTGACCCCTGCTACGCCTT
  CTTTGGAGTAGACGGCACTGAGTGCTCCTTCCTCATCTACCTCAGTGAGGTTGAGTGGTTCTGTCCCCCG
  TTGCCCTGGAGGAACCAGACAGCTGCCCGGACAGCCCCCAAGTCCCTTCCCAGAGTCCAGGCTGTGTTCC
  GAAGCAACCTGTCCCACCTCCTGGAGCTGATGGGCAGTGGGAAGGAGTCCCTCATCTTCATGAAGAAGCG
  AACCAGGCGGTTCACCGCACAGTGGACCAAGGCTGCCAAGTACCTGGCACAGAAGCTGGGGGACATTCGG
  AGGGACCAGAAGCAAATCCTTGTCCACATTGGCTTCCTGACAGAGGAGTCTGGGGACGTGTTCAGCCCAA
  GGGTACTGAAGGGCGGGCCTCTGGGAGAGATGGTACAGTGGGCAGACATCCTGGCTGCTCTCTACGTGCT
  GGGCCATAGCCTGCGGATCACAGTCTCCCTGAAGGAGCTGCAGAGTAACTTAGGGGTGCCGCCAGGCCGG
  GGGAACTGCCCACTCACCGTACCTCTGCCTTTTGACCTCATCTACACGGACTATCACGGCTTGCAGCAGA
  TGAAACAGCACATGGGACTGTCCTTCAAGAAGTACCGGTGCAGAATCCGAGTCATCGACACCTTTGGGAC
  GGAGCCAGCGTACAACCACGAGGAGTATGCCACGCTGCACGGCTACCGGACCAACTGGGGTTACTGGAAC
  CTCAACCCCAAGCAGTTCATGACCATGTTCCCTCACACCCCAGACAACTCCTTCATGGGCTTCGTGTCCG
  AGGAGCTCAATGAGACCGAGAAGCAGCTCATCAAAGATGGCAAGGCCAGCAACATGGCGGTGGTGTACGG
  CAAGGAGGCGAGTATCTGGAAGCTCCAGGGCAAGGAGAAGTTCCTGGCCGTCCTCAACAAGTACATGGAG
  ATCCACGGTACCGTGTACTATGAGAGCCAGCGGCCACCCGAGGTCCCCGCCTTCGTGAAGAACCACGGCC
  TCCTACCGCAGCCTGAGTTCCAGCAGCTGCTGCGGAAGGCCAAGCTCTTTATAGGGTTCGGATTCCCCTA
  CGAGGGCCCAGCACCGTTGGAAGCCATTGCCAATGGCTGCATCTTCCTACAGTCTCGCTTCAGCCCGCCC
  CACAGCTCCCTCAACCACGAGTTCTTCCGGGGCAAGCCCACCTCCAGGGAGGTGTTCTCCCAGCATCCGT
  ATGCAGAGAACTTTATTGGCAAGCCGCACGTGTGGACCGTGGACTATAACAACTCCGATGAGTTTGAAAC
  AGCCATTAAGGCCATCATGAACACCCAGGTAGACCCATATCTGCCCTATGAATATACCTGTGCAGGGATG
  CTGGAACGGATCAATGCCTACATCCAACACCAGGACTTCTGTGTGGGTCCAAGCCCTCTTCCACCAGGGG
  CCAGCACTGCCCAGAGTCCATTTGTCTTAGCTCCTAATGCAACTCATCTCGAGTGGGCCCAGAACATCAG
  CTCAGTTCCGGGAGCCTGGCCCCCTACCCACTCTCTGCGGGCCTGGCTGGCAGCCCCTGGAAGGGCCTGC
  ACGGACGCCTGCCTGGACCATGGATTGATCTGCGAGCCTTCCTTCTTCCCTTTCCTCAACAGCCAGAATT
  CGTTCCTCAAGCTGCAGGTGCCCTGTGACAGCACTGAGTGGGAGATGCATCACTTGTACCCTGCCTTTGC
  CCAACCCGGCCAAGAGTGCTACCTACAAAAAGAGCCACTGCTCTTCAGCTGTGCTGGTGCCAGCACCAAG
  TACCAGAGGCTCTGCCCCTGCCGTGACTTCCGCAAGGGTCAGGTGGCCTTGTGCCAGGGCTGCCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172948
• Insert Size: 2379 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172948.3, NP_766536.2
• RefSeq Size: 4277 bp
• RefSeq ORF: 2379 bp
• Locus ID: 268510
• UniProt ID: Q765H6
• Cytogenetics: 11 E2
• Gene Summary: Glycosyltransferase that acts on alpha-linked mannose of N-glycans and O-mannosyl glycans. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to the beta 1-6 linkage of the mannose residue of GlcNAc-beta1,2-Man-alpha on both the alpha1,3- and alpha1,6-linked mannose arms in the core structure of N-glycan (By similarity). Also acts on the GlcNAc-beta1,2-Man-alpha1-Ser/Thr moiety, forming a 2,6-branched structure in brain O-mannosyl glycan (PubMed:22715095). Plays an active role in modulating integrin and laminin-dependent adhesion and migration of neuronal cells via its activity in the O-mannosyl glycan pathway.[UniProtKB/Swiss-Prot Function]