Alkbh8 (NM_026303) Mouse Tagged ORF Clone

CAT#: MR209875

Alkbh8 (Myc-DDK-tagged) - Mouse alkB, alkylation repair homolog 8 (E. coli) (Alkbh8)

Product Images

Circular map for MR209875

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Alkbh8
• Synonyms: 4930562C03Rik; 8030431D03Rik; 9430088N01Rik; Abh8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209875 representing NM_026303
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATATCAATCATAAAGGTGTTTTGAAACTCACTAAAATGGAAAAGAAGTTCTTAAGGAAACAGAGTA
  AAGCCAGGCATGTGTTGCTGAAACATGAAGGCATCCAAGCAGTGTCTTATCCCACTCAGAGCCTGGTTAT
  TGCCAATGGTGGTCTGGGTAATGGCGTGAGTAGGAAACAACTGCTTTTGACTTTGGAGAAATGTGGACCT
  GTGGAAGCTCTCCTCATGCCACCTAATAAGCCATATGCATTTGTAATATTCCAAACTATTGAAGAATCCA
  AGAAAGCATATTTTACCCTCAATGGAAAAGAAATCATTGATGATTTGGGGCAGAAGATCTTTCTGTACTT
  GAATTTTGTTGAAAAAGCACAATGGAAAAACATGGGCCTTGAAGCCTTACCTCCAGGCCTCTTGGTGGTA
  GAAGAAATTATTTCTTCTGAGGAGGAGAAAAAGCTTCTGGAGAGTGTGAATTGGACAGAAGATACAGGCA
  ATCAGAATTTTCAAAGGTCCTTAAAACACAGAAGAGTGAAACATTTTGGCTATGAATTTCACTATGAGAG
  CAACACTGTGGATAAAGACAAGCCCTTACCTGGGGGTCTTCCTGAGGTTTGCAGTAGCATTTTGGAGAAG
  TTGTTGAAAGAAGGTTATATTAAACATAAACCAGACCAGTTGACCATAAATCAATATGAACCTGGGCATG
  GAATTCCTGCCCATATTGACACGCATTCTGCATTTGAAGATGAGATTATTTCTCTCAGTTTGGGGTCAGC
  GATTGTTATGGATTTCAAGCATCCAGAGGGTGTCACAGTACAAGTTATGTTACCTCGTCGGAGTTTGCTG
  GTGATGACAGGAGAATCTAGATACCTTTGGACTCATGGAATTACACCTCGAAAATTTGATACCGTCCAAG
  CGTCTGAGCAATTTAAAGGGGGAATAATCACCAGTGACATTGGAGACTTGACTTTAAGCAAGAGGGGAAT
  GCGCACATCCTTTACGTTTAGAAAAGTGAGGCGAATGCCCTGCAATTGTAGTTACTCCTCAGTCTGCGAC
  AGACAAAGGAAGGCCACACCTCCATCCCTTACAGAGAGCAGTAAAGAAGCATTGGAGTTAGAGCAAAAGC
  ATGTCCATCAAGTGTACAATGAGATTGCAAGTCACTTCAGCAGCACAAGACATAGCCCATGGCCACGCAT
  AGTAGAGTTCCTGAAAGCTTTGCCAAGTGGTTCCATAGTGGCAGATATCGGATGTGGAAATGGAAAATAC
  CTTGGCATCAACAAGGACTTATATATGATTGGGTGTGATCGTAGCCAGAACCTTGTGGACATTTGTAGAG
  AGAGGCAGTTCCAGGCCTTAGTCTGCGATGCGCTGGCTGTTCCTGTCAGAAGTGGGTCTTGTGATGCTTG
  CATCTCTATTGCCGTCATTCACCACTTTGCAACTGCAGAGCGCAGAGTGGAAGCTCTCCAAGAACTTGCC
  CGTCTCCTGAGACCTGGTGGGCAGGCCCTCATTTATGTCTGGGCAATGGAACAAGAGTATAAAAATCAGA
  AGTCCAAGTATCTTAGAGGAAAGAGAATTAGCCAAGGAGATAAAGATGAGCTAAACAGTGCCACATCTAC
  GGAAGAATTTCTAGTAAATCAAACGCCTGAGGGGGTCAATGAAGACCCAGCATTGTCTGTCAACTCCAGT
  AGTATCACCAAGGAAGAAGAGTATAAGTCAAGGAAAGTTCCTAATTCTGAACTCCCTATTCATATAAATA
  GGACCTGCTTTCATTCCCAAGATGTGCTGGTTCCCTGGCACCTCAAAAGAAACCCTGGTAAAGACAAAGC
  TATTGAACCATCTGGTGTAGCAGGATGTCCTGACCCAAGTCCTGTGTTTCATCGTTATTACCATGTTTTC
  TGTGATGGAGAATTGGAAGCTTCATGCCAGGCTGTGGGTGATGTTAGCATTCTGCAGAGCTATTACGATC
  AAGGGAATTGGTGTGTTGTTCTTCAAAAGGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209875 representing NM_026303
  Red=Cloning site Green=Tags(s)
  
  MNINHKGVLKLTKMEKKFLRKQSKARHVLLKHEGIQAVSYPTQSLVIANGGLGNGVSRKQLLLTLEKCGP
  VEALLMPPNKPYAFVIFQTIEESKKAYFTLNGKEIIDDLGQKIFLYLNFVEKAQWKNMGLEALPPGLLVV
  EEIISSEEEKKLLESVNWTEDTGNQNFQRSLKHRRVKHFGYEFHYESNTVDKDKPLPGGLPEVCSSILEK
  LLKEGYIKHKPDQLTINQYEPGHGIPAHIDTHSAFEDEIISLSLGSAIVMDFKHPEGVTVQVMLPRRSLL
  VMTGESRYLWTHGITPRKFDTVQASEQFKGGIITSDIGDLTLSKRGMRTSFTFRKVRRMPCNCSYSSVCD
  RQRKATPPSLTESSKEALELEQKHVHQVYNEIASHFSSTRHSPWPRIVEFLKALPSGSIVADIGCGNGKY
  LGINKDLYMIGCDRSQNLVDICRERQFQALVCDALAVPVRSGSCDACISIAVIHHFATAERRVEALQELA
  RLLRPGGQALIYVWAMEQEYKNQKSKYLRGKRISQGDKDELNSATSTEEFLVNQTPEGVNEDPALSVNSS
  SITKEEEYKSRKVPNSELPIHINRTCFHSQDVLVPWHLKRNPGKDKAIEPSGVAGCPDPSPVFHRYYHVF
  CDGELEASCQAVGDVSILQSYYDQGNWCVVLQKV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026303
• ORF Size: 1992 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026303.1, NP_080579.1
• RefSeq Size: 2300 bp
• RefSeq ORF: 1995 bp
• Locus ID: 67667
• UniProt ID: Q80Y20
• Cytogenetics: 9 A1
• MW: 74.8 kDa
• Gene Summary: Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain (PubMed:20123966). Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA (PubMed:20123966). Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys) (By similarity). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine; has a preference for tRNA(Gly) (PubMed:20583019). Required for normal survival after DNA damage (By similarity). May inhibit apoptosis and promote cell survival and angiogenesis (By similarity).[UniProtKB/Swiss-Prot Function]