Alkbh8 (NM_026303) Mouse Untagged Clone

CAT#: MC204641

Alkbh8 (untagged) - Mouse alkB, alkylation repair homolog 8 (E. coli) (Alkbh8), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Alkbh8
• Synonyms: 4930562C03Rik; 8030431D03Rik; 9430088N01Rik; Abh8
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC050863
  GATTGGTGTGGTTTACACAGTATTACTATGAATATCAATCATAAAGGTGTTTTGAAACTCACTAAAATGG AAAAGAAGTTCTTAAGGAAACAGAGTAAAGCCAGGCATGTGTTGCTGAAACATGAAGGCATCCAAGCAGT GTCTTATCCCACTCAGAGCCTGGTTATTGCCAATGGTGGTCTGGGTAATGGCGTGAGTAGGAAACAACTG CTTTTGACTTTGGAGAAATGTGGACCTGTGGAAGCTCTCCTCATGCCACCTAATAAGCCATATGCATTTG TAATATTCCAAACTATTGAAGAATCCAAGAAAGCATATTTTACCCTCAATGGAAAAGAAATCATTGATGA TTTGGGGCAGAAGATCTTTCTGTACTTGAATTTTGTTGAAAAAGCACAATGGAAAAACATGGGCCTTGAA GCCTTACCTCCAGGCCTCTTGGTGGTAGAAGAAATTATTTCTTCTGAGGAGGAGAAAAAGCTTCTGGAGA GTGTGAATTGGACAGAAGATACAGGCAATCAGAATTTTCAAAGGTCCTTAAAACACAGAAGAGTGAAACA TTTTGGCTATGAATTTCACTATGAGAGCAACACTGTGGATAAAGACAAGCCCTTACCTGGGGGTCTTCCT GAGGTTTGCAGTAGCATTTTGGAGAAGTTGTTGAAAGAAGGTTATATTAAACATAAACCAGACCAGTTGA CCATAAATCAATATGAACCTGGGCATGGAATTCCTGCCCATATTGACACGCATTCTGCATTTGAAGATGA GATTATTTCTCTCAGTTTGGGGTCAGCGATTGTTATGGATTTCAAGCATCCAGAGGGTGTCACAGTACAA GTTATGTTACCTCGTCGGAGTTTGCTGGTGATGACAGGAGAATCTAGATACCTTTGGACTCATGGAATTA CACCTCGAAAATTTGATACCGTCCAAGCGTCTGAGCAATTTAAAGGGGGAATAATCACCAGTGACATTGG AGACTTGACTTTAAGCAAGAGGGGAATGCGCACATCCTTTACGTTTAGAAAAGTGAGGCGAATGCCCTGC AATTGTAGTTACTCCTCAGTCTGCGACAGACAAAGGAAGGCCACACCTCCATCCCTTACAGAGAGCAGTA AAGAAGCATTGGAGTTAGAGCAAAAGCATGTCCATCAAGTGTACAATGAGATTGCAAGTCACTTCAGCAG CACAAGACATAGCCCATGGCCACGCATAGTAGAGTTCCTGAAAGCTTTGCCAAGTGGTTCCATAGTGGCA GATATCGGATGTGGAAATGGAAAATACCTTGGCATCAACAAGGACTTATATATGATTGGGTGTGATCGTA GCCAGAACCTTGTGGACATTTGTAGAGAGAGGCAGTTCCAGGCCTTAGTCTGCGATGCGCTGGCTGTTCC TGTCAGAAGTGGGTCTTGTGATGCTTGCATCTCTATTGCCGTCATTCACCACTTTGCAACTGCAGAGCGC AGAGTGGAAGCTCTCCAAGAACTTGCCCGTCTCCTGAGACCTGGTGGGCAGGCCCTCATTTATGTCTGGG CAATGGAACAAGAGTATAAAAATCAGAAGTCCAAGTATCTTAGAGGAAAGAGAATTAGCCAAGGAGATAA AGATGAGCTAAACAGTGCCACATCTACGGAAGAATTTCTAGTAAATCAAACGCCTGAGGGGGTCAATGAA GACCCAGCATTGTCTGTCAACTCCAGTAGTATCACCAAGGAAGAAGAGTATAAGTCAAGGAAAGTTCCTA ATTCTGAACTCCCTATTCATATAAATAGGACCTGCTTTCATTCCCAAGATGTGCTGGTTCCCTGGCACCT CAAAAGAAACCCTGGTAAAGACAAAGCTATTGAACCATCTGGTGTAGCAGGATGTCCTGACCCAAGTCCT GTGTTTCATCGTTATTACCATGTTTTCTGTGATGGAGAATTGGAAGCTTCATGCCAGGCTGTGGGTGATG TTAGCATTCTGCAGAGCTATTACGATCAAGGGAATTGGTGTGTTGTTCTTCAAAAGGTCTGATCACTTAT CTGAACATATCATGTGTAAAGAGAAAAAAAAAAGTCAATCTCTTTTTGAAAAGGAAACTAAATTATCTCT TTAAATAAGGAGGAAATTTGGAACAGAGATACTTAGGAAACATTTTTCTCTATTCCCGTTGTCTGACTTC ATAAACGTAGGTTCCTCTTACATGCCTTTTAAGTATGGTGTTTGACATTAACTTGTGAAAAGTCTGTGAT GTTTGTATATATATAGCATTTGAAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_026303
• Insert Size: 1995 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC050863, AAH50863
• RefSeq Size: 2300 bp
• RefSeq ORF: 1995 bp
• Locus ID: 67667
• UniProt ID: Q80Y20
• Cytogenetics: 9 A1
• Gene Summary: Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain (PubMed:20123966). Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA (PubMed:20123966). Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys) (By similarity). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine; has a preference for tRNA(Gly) (PubMed:20583019). Required for normal survival after DNA damage (By similarity). May inhibit apoptosis and promote cell survival and angiogenesis (By similarity).[UniProtKB/Swiss-Prot Function]