Glis1 (BC066157) Mouse Tagged ORF Clone

CAT#: MR209472

Glis1 (Myc-DDK-tagged) - Mouse GLIS family zinc finger 1 (cDNA clone MGC:76505 IMAGE:30468539)

Product Images

Circular map for MR209472

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Glis1
• Synonyms: Gli6, GliH1, Gli5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209472 representing BC066157
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCAGCGCCCACACACCCCTGCCCACCCACTGCCGAGCCCCATCGTCCATGGGTCTGCCCTCAGACC
  TGGACTTTCCAGACCGAGGCCTCACCAACCCTGCACCTTCCTGCTACCTTCTGGGCAATGAACCCATCTC
  AGACCTGGGTCCCCAACCCGAGGCCCACCTCCCCGAGGGCAGCCTGAAACGCTGCTGCCTCCTGGGCCTG
  CCCCCCACCTCTTCAGCCTCCTCCTCACCCTGTGCCTCCTCAGATATCAATCCTGTCATCCACTCCTCCC
  AGACAGCTCTAGTTAGCTGTGTAAATGGACTCCGAAGCCCACCTCTGCCGGGAGACCTGGGGGGCCCTCC
  CAAGCGGTCACGGCCCGGGCCTGCATCCAGTGACGGCCAGGAGGGCAGCTTGCAGCTTGAAGCATGCCGG
  AAGTCAGGCTTCCTGAAGCAGGAGCCCATGGACGAGTTTTCAGAGCTTTTTGCTCCACACCACCAGGGTT
  TGCCACCCCCTTACCCCTTGCCTCAGTTGCCAACTGGCCCCGGCCTCGGAGGCCTAGGGCTGGGCCTGGC
  AGGTAGGATGGTTGCCGGTCGGCAGGCATGCCGCTGGGTGGACTGCTGCGCAGCCTACGGGCAGCAGGAG
  GAGCTGGAGCGGCACATCGAGAAGAGCCACATCGACCAGCGCAAGGGCGAAGACTTCACCTGCTTCTGGG
  CCGGGTGTGTGCGGCGCTACAAGCCCTTCAATGCCCGCTACAAGCTGCTCATCCACATGAGGGTACACTC
  AGGCGAGAAGCCCAACAAGTGCATGTTCGAAGGCTGCAGTAAAGCCTTTTCCCGTCTGGAGAACCTGAAG
  ATCCATCTGCGGAGCCACACAGGCGAGAAACCATACCTGTGCCAGCACCCAGGCTGCCAGAAGGCCTTCA
  GCAACTCCAGCGACCGTGCCAAGCACCAACGCACCCACCTCGACACGAAGCCATATGCTTGTCAGATCCC
  TGGCTGCTCCAAGCGCTACACGGACCCCAGCTCCCTCCGCAAGCACGTGAAGGCCCACTCAGCCAAAGAG
  CAGCAGGTGCGTAAGAAGCTGCACACAGGTGCCGACCCAGAGGCTGATGTTCTGTCCGAGTGTCTGTCCC
  TGCAGCAGCTCCAAGCATCCACACTGTTGCCGGCCAGCAGAGGGAAGGGCAGCCAAACCCTGAGCCAGGA
  GCTCCTCCCAGGTGTGTATCCTGGCTCCGTCACCCCACAAAACGGGCTTGCTTCAGGCATCCTGTCCCCC
  TCCCACGATGTCCCTTCCAGGCACCACCCACTGGAGGTCCCCACTGGTTCCCACCACCACCTGTCCCCTC
  TGCCCACAGCTGAGAGCACCAGGGATGGCCTGGGGCCCAGTCTCCTTTCACCCATGGCCAGCCCACTGAA
  GGGGCTTGGTCCCCCACCGCTACCACCAGCCTCCCAGAGTCAGTCTCCAGGGGGACAGTCATTCTCTACA
  GTCCCCAGCAAGCCTACCTCCCCATCCTTCCAAAGCCCACCACCTCTGCCCAGCCCCCAAGGCTACCAAG
  GCAGTTTCCATTCCATCCAGAACTGCTTCCCCTACGCTGACTGCTACCGGGCCACTGAGCCAGCAGCCTC
  CAGGGATGGACTGGTGGGTGATGCCCACGGTTTCAACCCCTTGCGACCCAGCACATACTCCAGCCTCAGC
  ACACCTTTATCCGCACCAGGCTACGAGACCCTGGCAGAAACGCCGTGTCCCCCAGCGCTGCAGCCACAGC
  CAGCTGAAGACCTGGTACCTAGTGGTCCTGAGGACTGTGGCTTCTTCCCCAATGGGGCCTTTGACCACTG
  TCTGAGTCACATCCCGTCCATCTACACTGACACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209472 representing BC066157
  Red=Cloning site Green=Tags(s)
  
  MVSAHTPLPTHCRAPSSMGLPSDLDFPDRGLTNPAPSCYLLGNEPISDLGPQPEAHLPEGSLKRCCLLGL
  PPTSSASSSPCASSDINPVIHSSQTALVSCVNGLRSPPLPGDLGGPPKRSRPGPASSDGQEGSLQLEACR
  KSGFLKQEPMDEFSELFAPHHQGLPPPYPLPQLPTGPGLGGLGLGLAGRMVAGRQACRWVDCCAAYGQQE
  ELERHIEKSHIDQRKGEDFTCFWAGCVRRYKPFNARYKLLIHMRVHSGEKPNKCMFEGCSKAFSRLENLK
  IHLRSHTGEKPYLCQHPGCQKAFSNSSDRAKHQRTHLDTKPYACQIPGCSKRYTDPSSLRKHVKAHSAKE
  QQVRKKLHTGADPEADVLSECLSLQQLQASTLLPASRGKGSQTLSQELLPGVYPGSVTPQNGLASGILSP
  SHDVPSRHHPLEVPTGSHHHLSPLPTAESTRDGLGPSLLSPMASPLKGLGPPPLPPASQSQSPGGQSFST
  VPSKPTSPSFQSPPPLPSPQGYQGSFHSIQNCFPYADCYRATEPAASRDGLVGDAHGFNPLRPSTYSSLS
  TPLSAPGYETLAETPCPPALQPQPAEDLVPSGPEDCGFFPNGAFDHCLSHIPSIYTDT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC066157
• ORF Size: 1854 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC066157.1
• RefSeq Size: 2431 bp
• RefSeq ORF: 1856 bp
• Locus ID: 230587
• Cytogenetics: 4 C7
• MW: 89.1 kDa
• Gene Summary: Acts as both a repressor and activator of transcription (PubMed:12042312, PubMed:12385751, PubMed:21654807). Binds to the consensus sequence 5'-GACCACCCAC-3' (PubMed:12042312). By controlling the expression of genes involved in cell differentiation inhibits the lineage commitment of multipotent cells (PubMed:21654807, PubMed:30544251). Prevents, for instance, the differentiation of multipotent mesenchymal cells into adipocyte and osteoblast (PubMed:30544251).[UniProtKB/Swiss-Prot Function]