Glis1 (BC066157) Mouse Untagged Clone

CAT#: MC218609

Glis1 (untagged) - Mouse GLIS family zinc finger 1 (cDNA clone MGC:76505 IMAGE:30468539), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Glis1
• Synonyms: Gli6, GliH1, Gli5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218609 representing BC066157
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCAGCGCCCACACACCCCTGCCCACCCACTGCCGAGCCCCATCGTCCATGGGTCTGCCCTCAGACC
  TGGACTTTCCAGACCGAGGCCTCACCAACCCTGCACCTTCCTGCTACCTTCTGGGCAATGAACCCATCTC
  AGACCTGGGTCCCCAACCCGAGGCCCACCTCCCCGAGGGCAGCCTGAAACGCTGCTGCCTCCTGGGCCTG
  CCCCCCACCTCTTCAGCCTCCTCCTCACCCTGTGCCTCCTCAGATATCAATCCTGTCATCCACTCCTCCC
  AGACAGCTCTAGTTAGCTGTGTAAATGGACTCCGAAGCCCACCTCTGCCGGGAGACCTGGGGGGCCCTCC
  CAAGCGGTCACGGCCCGGGCCTGCATCCAGTGACGGCCAGGAGGGCAGCTTGCAGCTTGAAGCATGCCGG
  AAGTCAGGCTTCCTGAAGCAGGAGCCCATGGACGAGTTTTCAGAGCTTTTTGCTCCACACCACCAGGGTT
  TGCCACCCCCTTACCCCTTGCCTCAGTTGCCAACTGGCCCCGGCCTCGGAGGCCTAGGGCTGGGCCTGGC
  AGGTAGGATGGTTGCCGGTCGGCAGGCATGCCGCTGGGTGGACTGCTGCGCAGCCTACGGGCAGCAGGAG
  GAGCTGGAGCGGCACATCGAGAAGAGCCACATCGACCAGCGCAAGGGCGAAGACTTCACCTGCTTCTGGG
  CCGGGTGTGTGCGGCGCTACAAGCCCTTCAATGCCCGCTACAAGCTGCTCATCCACATGAGGGTACACTC
  AGGCGAGAAGCCCAACAAGTGCATGTTCGAAGGCTGCAGTAAAGCCTTTTCCCGTCTGGAGAACCTGAAG
  ATCCATCTGCGGAGCCACACAGGCGAGAAACCATACCTGTGCCAGCACCCAGGCTGCCAGAAGGCCTTCA
  GCAACTCCAGCGACCGTGCCAAGCACCAACGCACCCACCTCGACACGAAGCCATATGCTTGTCAGATCCC
  TGGCTGCTCCAAGCGCTACACGGACCCCAGCTCCCTCCGCAAGCACGTGAAGGCCCACTCAGCCAAAGAG
  CAGCAGGTGCGTAAGAAGCTGCACACAGGTGCCGACCCAGAGGCTGATGTTCTGTCCGAGTGTCTGTCCC
  TGCAGCAGCTCCAAGCATCCACACTGTTGCCGGCCAGCAGAGGGAAGGGCAGCCAAACCCTGAGCCAGGA
  GCTCCTCCCAGGTGTGTATCCTGGCTCCGTCACCCCACAAAACGGGCTTGCTTCAGGCATCCTGTCCCCC
  TCCCACGATGTCCCTTCCAGGCACCACCCACTGGAGGTCCCCACTGGTTCCCACCACCACCTGTCCCCTC
  TGCCCACAGCTGAGAGCACCAGGGATGGCCTGGGGCCCAGTCTCCTTTCACCCATGGCCAGCCCACTGAA
  GGGGCTTGGTCCCCCACCGCTACCACCAGCCTCCCAGAGTCAGTCTCCAGGGGGACAGTCATTCTCTACA
  GTCCCCAGCAAGCCTACCTCCCCATCCTTCCAAAGCCCACCACCTCTGCCCAGCCCCCAAGGCTACCAAG
  GCAGTTTCCATTCCATCCAGAACTGCTTCCCCTACGCTGACTGCTACCGGGCCACTGAGCCAGCAGCCTC
  CAGGGATGGACTGGTGGGTGATGCCCACGGTTTCAACCCCTTGCGACCCAGCACATACTCCAGCCTCAGC
  ACACCTTTATCCGCACCAGGCTACGAGACCCTGGCAGAAACGCCGTGTCCCCCAGCGCTGCAGCCACAGC
  CAGCTGAAGACCTGGTACCTAGTGGTCCTGAGGACTGTGGCTTCTTCCCCAATGGGGCCTTTGACCACTG
  TCTGAGTCACATCCCGTCCATCTACACTGACACCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC066157
• Insert Size: 1857 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC066157, AAH66157
• RefSeq Size: 2431 bp
• RefSeq ORF: 1856 bp
• Locus ID: 230587
• Cytogenetics: 4 C7
• Gene Summary: Acts as both a repressor and activator of transcription (PubMed:12042312, PubMed:12385751, PubMed:21654807). Binds to the consensus sequence 5'-GACCACCCAC-3' (PubMed:12042312). By controlling the expression of genes involved in cell differentiation inhibits the lineage commitment of multipotent cells (PubMed:21654807, PubMed:30544251). Prevents, for instance, the differentiation of multipotent mesenchymal cells into adipocyte and osteoblast (PubMed:30544251).[UniProtKB/Swiss-Prot Function]