Mtmr6 (NM_144843) Mouse Tagged ORF Clone

CAT#: MR209453

Mtmr6 (Myc-DDK-tagged) - Mouse myotubularin related protein 6 (Mtmr6)

Product Images

Circular map for MR209453

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mtmr6
• Synonyms: 4022440C11Rik; AI428804; AU041072
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209453 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCACATCCGGACAACCAAGGTTGAACAAGTAAAGTTACTTGACCGGTTTAGTACCAACAACAAGT
  CACTGACAGGAACACTGTATCTTACGGCCACACACCTATTATTTATTGACGCTCAGCAAAAAGAAACCTG
  GATACTACACCACCATATTGCCTCTGTGGAGAAACTTGCTCTGACCACTTCTGGATGCCCACTTGTGATT
  CAGTGCAAGAACTTCAGGATTGTGCATTTCATTGTTCCCAGGGAAAGAGATTGCCACGATATTTATAACT
  CTCTCCTGCAGCTATCAAAACAAGCAAAATATGAAGATCTCTATGCCTTCTCTTATAATCCCAAGCAAAA
  TGATACTGAACGACGGAATGGTTGGCAGCTTATTGACCTTGCTGCGGAGTATGAGAGGATGGGAGTGCCC
  AATGCAAACTGGCAACTGTCGGATGCCAACCGAGAGTACAAGGTCTGTGAGACTTATCCCAGAGAGCTGT
  ATGTTCCTCGGACAGCTAGCCGGCCGGTGATCGTGGGCAGTTCCAACTTCCGGAGCAAGGGCAGACTTCC
  GGTCCTATCCTACTGCCGGCAGGGCACTGAGGCTGCCATTTGTCGATGTAGTCAGCCATTGTCAGGCTTC
  AGTGCCAGGTGCCTAGAGGATGAGCATTTGCTTCAAGCCATTAGTAAAGCCAACCCAGGAAACCGCTACA
  TGTATGTTGTGGATACCAGGCCCAAGCTCAATGCAATAGCTAACAGAGCAGCTGGAAAAGGTTATGAAAA
  TGAAGACAACTATTCTAATATTAGATTTCAGTTCGTTGGAATAGAAAATATTCATGTCATGAGATCCAGC
  CTTCAGAAATTACTGGAAGTGAATGGTAGCAAGGGGCTTTCTGTCAATGATTTCTACTCTGGTTTGGAAA
  GTTCAGGATGGCTTCGCCATATCAAGGCTGTTTTGGATGCTGCAATCTTCTTAGCCAAAGCAATAGTGGT
  TGAAAATGCAAGTGTGTTGGTCCACTGTTCCGATGGGTGGGACAGGACCTCCCAAGTGTGCTCCCTCGGC
  TCACTGTTGTTGGATTCCTACTACAGGACAATGAAAGGATTCATGGTTTTAATAGAAAAAGATTGGATAT
  CATTTGGGCATAAATTTTCAGAGAGGTGTGGCCATTTGGATGGAGACCCGAGGGAGGTCTCACCAGTGTT
  CACTCAGTTCTTGGAGTGTGTCTGGCACCTGACTCAGCAGTTCCCACAAGCCTTCGAGTTCAACGAAGCG
  TTTCTTCTTCAGATCCATGAGCACATTCATTCATGCCAATTTGGGAACTTCCTTGGCAACTGTCAGAAGG
  AGAGAGAGGAACTCAGGTTAAAGGAGAAAACTTACTCCCTGTGGCCTTTTCTTTTGGATGACAAAAAGAA
  GTACTTAAATCCTCTCTACAGTTCCAAATCTCAGAGACTGACAGTCTTGGAGCCAAATACAGCCTCTTTC
  AATTTTAAGTTTTGGAGAAACATGTACCACCAGTTTGATCGGACGCTCCATCCTCGGCAGTCTGTGCTGA
  GCATAATTATGAATATGAATGAGCAAAGCAAGCAGTTAGAGGAAGACATTAAAGACCTCGAGGCTAAAAT
  TAAGCAATGCAAAAATGGCATCCTAACGAAGGAGTTGCTGCATGCTGTCCACCCTGAATCACCTGCCCTG
  AAAACCTCGCTGTGTCTCAAAGAGCCGAGTCTGCTTCCCGTGAAGGACACACTCCGAGCTATAGAGGGCA
  GCAGCCCAGCAGATAATCGCTACTGTGACTATGCCGAGGAATTTTCTAAGTCGGAGCCTACCGTGGTCAG
  CTTGGAGTACGGGGTGGCCAGAATGACTTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209453 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEHIRTTKVEQVKLLDRFSTNNKSLTGTLYLTATHLLFIDAQQKETWILHHHIASVEKLALTTSGCPLVI
  QCKNFRIVHFIVPRERDCHDIYNSLLQLSKQAKYEDLYAFSYNPKQNDTERRNGWQLIDLAAEYERMGVP
  NANWQLSDANREYKVCETYPRELYVPRTASRPVIVGSSNFRSKGRLPVLSYCRQGTEAAICRCSQPLSGF
  SARCLEDEHLLQAISKANPGNRYMYVVDTRPKLNAIANRAAGKGYENEDNYSNIRFQFVGIENIHVMRSS
  LQKLLEVNGSKGLSVNDFYSGLESSGWLRHIKAVLDAAIFLAKAIVVENASVLVHCSDGWDRTSQVCSLG
  SLLLDSYYRTMKGFMVLIEKDWISFGHKFSERCGHLDGDPREVSPVFTQFLECVWHLTQQFPQAFEFNEA
  FLLQIHEHIHSCQFGNFLGNCQKEREELRLKEKTYSLWPFLLDDKKKYLNPLYSSKSQRLTVLEPNTASF
  NFKFWRNMYHQFDRTLHPRQSVLSIIMNMNEQSKQLEEDIKDLEAKIKQCKNGILTKELLHAVHPESPAL
  KTSLCLKEPSLLPVKDTLRAIEGSSPADNRYCDYAEEFSKSEPTVVSLEYGVARMTC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144843
• ORF Size: 1854 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144843.4
• RefSeq Size: 3845 bp
• RefSeq ORF: 1854 bp
• Locus ID: 219135
• UniProt ID: Q8VE11
• Cytogenetics: 14 D1
• MW: 70.9 kDa
• Gene Summary: Phosphatase that acts on lipids with a phosphoinositol headgroup. Dephosphorylates phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 3,5-bisphosphate. Binds with high affinity to phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) but also to phosphatidylinositol 3-phosphate (PtdIns(3)P), phosphatidylinositol 4-phosphate (PtdIns(4)P), and phosphatidylinositol 5-phosphate (PtdIns(5)P), phosphatidic acid and phosphatidylserine (By similarity). Negatively regulates ER-Golgi protein transport (By similarity). Probably in association with MTMR9, plays a role in the late stages of macropinocytosis by dephosphorylating phosphatidylinositol 3-phosphate in membrane ruffles (PubMed:24591580). Acts as a negative regulator of KCNN4/KCa3.1 channel activity in CD4(+) T-cells possibly by decreasing intracellular levels of phosphatidylinositol 3-phosphate. Negatively regulates proliferation of reactivated CD4(+) T-cells. In complex with MTMR9, negatively regulates DNA damage-induced apoptosis. The formation of the MTMR6-MTMR9 complex stabilizes both MTMR6 and MTMR9 protein levels (By similarity).[UniProtKB/Swiss-Prot Function]