Onecut3 (NM_139226) Mouse Tagged ORF Clone

CAT#: MR207851

Onecut3 (Myc-DDK-tagged) - Mouse one cut domain, family member 3 (Onecut3)

Product Images

Circular map for MR207851

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Onecut3
• Synonyms: OC-3; Oc3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207851 representing NM_139226
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTGAGCTTGGAGAGCCTCGGGGGGCTGCACGGGGTGACACACGCGCAAGCCGGCGAGCTGCTGA
  GCCCGGGCCACGCGCGCTCCGCCGCGGCCCAACACCGAAGCCTAGTGGCATCGGGGCGCCCAGGCCTGGT
  GGCCGGCATGGCTAGCCTGCTGGACGGTGGTGGCGCCGGGGGCGGGGGCGCCGGGGGCGCGGGGGCTGCA
  GGGGCCGCGGGTGGTGGCCCTGACTTCCGTGGGGAGCTGGCTGGCCCCCTGCACCCGGCCATGGGTATGG
  CGTGCGAGGCCCCAGGTTTGGGTGGCACCTACACCACGCTCACGCCCTTGCAGCACCTGCCGCCGCTGGC
  CGCCGTGGCCGATAAGTTCCACCAGCACGCGGTGGCTGGGGCGCACGGAGGCCATCCACACGCTCACCCT
  CACCCGGCCACCGCGCCGCCACCGCCCCCTCCGCAGCGCCTGGCGGCCAGCGTGAGCGGCAGCTTCACCC
  TCATGCGCGACGAGCGGGCGGCGCTGGCCTCGGTGGGCCACCTCTACGGGCCTTATGGCAAGGAGCTGCC
  CACCATGGGGTCGCCACTGTCGCCGCTGCCCAGCGCGCTGCCCCCCGCTCTGCACAGCGCCCCGCAGCCG
  CCGCCGCCGCCACCGCTCGCCGCTTACGGGGCTCCGGGCCACCTGGCCGGGGACAAACTACTGCCACCTG
  CCGCCTTCGAGCCACACGCGGCGCTTCTGGGGCGCGCAGAGGACGCGCTGGCCCGAGGGCTGCCTGGAGG
  CGGCGGCGGCGCGGGAGGCGGCGGGGCGGCCGGCGGGGCGGCCGCGGGTTTGCTGGCTCCGCTGGGCGGG
  CTGGCTGCGGCCGGGGCGCACGGGCCTCACTCAGGCGGCGGTGGCCCCGGAGGGGGCGGTGGCGCTGGCG
  GCGGCAGCGGCGGCCCGGGAGCCGGCGCTGCAGCCGAAGAGATCAACACCAAAGAGGTGGCCCAGCGCAT
  CACCGCCGAGCTGAAGCGCTACAGCATCCCGCAGGCCATCTTCGCGCAGCGCATCCTGTGCCGCTCGCAG
  GGCACGCTCTCCGACCTGCTGCGCAACCCGAAGCCCTGGAGCAAGCTCAAATCCGGCCGCGAGACCTTCC
  GCAGGATGTGGAAGTGGCTGCAGGAGCCGGAGTTCCAGCGCATGTCGGCGCTGCGCCTGGCAGCCTGCAA
  GCGTAAAGAGCAGGATCAGCAGAAGGAGAGGGCTCTGCAGCCGAAGAAGCAACGCCTCGTGTTCACAGAC
  CTGCAGCGCCGCACGCTGATTGCCATCTTCAAGGAGAACAAGCGGCCGTCCAAGGAGATGCAAGCCACCA
  TCTCGCAGCAGCTGGGCCTGGAGCTCAACACTGTCAGCAACTTCTTCATGAACGCCCGTCGCCGCTGCAT
  GAACCGCTGGGCTGAGGAGCCGGGTGCCACCCCTGGCACCGGGACAGCTACTGCCACCTTCTCCAAGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207851 representing NM_139226
  Red=Cloning site Green=Tags(s)
  
  MELSLESLGGLHGVTHAQAGELLSPGHARSAAAQHRSLVASGRPGLVAGMASLLDGGGAGGGGAGGAGAA
  GAAGGGPDFRGELAGPLHPAMGMACEAPGLGGTYTTLTPLQHLPPLAAVADKFHQHAVAGAHGGHPHAHP
  HPATAPPPPPPQRLAASVSGSFTLMRDERAALASVGHLYGPYGKELPTMGSPLSPLPSALPPALHSAPQP
  PPPPPLAAYGAPGHLAGDKLLPPAAFEPHAALLGRAEDALARGLPGGGGGAGGGGAAGGAAAGLLAPLGG
  LAAAGAHGPHSGGGGPGGGGGAGGGSGGPGAGAAAEEINTKEVAQRITAELKRYSIPQAIFAQRILCRSQ
  GTLSDLLRNPKPWSKLKSGRETFRRMWKWLQEPEFQRMSALRLAACKRKEQDQQKERALQPKKQRLVFTD
  LQRRTLIAIFKENKRPSKEMQATISQQLGLELNTVSNFFMNARRRCMNRWAEEPGATPGTGTATATFSKA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139226
• ORF Size: 1470 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139226.3, NP_631972.2
• RefSeq Size: 4705 bp
• RefSeq ORF: 1473 bp
• Locus ID: 246086
• UniProt ID: Q8K557
• Cytogenetics: 10 C1
• MW: 49.6 kDa
• Gene Summary: Transcriptional activator. Binds the consensus DNA sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR.[UniProtKB/Swiss-Prot Function]