Lpcat3 (NM_145130) Mouse Tagged ORF Clone

CAT#: MR207801

Lpcat3 (Myc-DDK-tagged) - Mouse lysophosphatidylcholine acyltransferase 3 (Lpcat3)

Product Images

Circular map for MR207801

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lpcat3
• Synonyms: C3f; Grcc3f; Lpcat; Lpeat; Lplat5; Lpsat; Mboat5; Moact5; Oact5; PTG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207801 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCTACAGCGGACGGGGACATGGGAGAGACGCTGGAGCAGATGCGGGGGCTGTGGCCGGGTGTCG
  AGGATCTGAGCCTTAACAAGTTGGCGACGTCTCTGGGCGCGTCGGAACAGGCGCTGCGGCTCATCTTCTC
  CATCTTCCTGGGCTACCCGTTGGCTCTGTTTTACCGGCATTACCTTTTCTACAAGGACAGCTACCTCATC
  CATCTCTTCCACACCTTCACGGGCCTCTCAATTGCTTATTTCAACTTTGGCCACCAGTTCTACCACTCCT
  TGCTATGTGTCGTGCTTCAGTTCCTCATCCTGCGACTCATGGGCCGCACCGTCACTGCCGTTATTACTAC
  CCTTTGCTTCCAGATGGCCTACCTTCTTGCCGGATATTACTACACAGCCACCGGTGACTACGATATCAAG
  TGGACAATGCCACATTGTGTCTTGACACTGAAGCTAATTGGGCTGTGTATTGACTACTACGATGGAGGCA
  AAGACGGGAATTCCTTGACCTCTGAGCAACAGAAATATGCCATACGGGGTGTCCCTTCATTGCTGGAAGT
  TGCTGGCTTCTCCTACTTCTATGGAGCCTTCTTGGTAGGGCCCCAATTTTCAATGAACCACTACATGAAG
  CTGGTGCGGGGACAGCTGACTGACATACCAGGGAAGATGCCAAACAGCACCATACCTGCTCTCAAGCGCC
  TGAGTCTGGGCCTTGTCTACCTGGTGGGCTACACCCTGCTGAGCCCCCACATCACAGACGACTATCTCCT
  CACAGAAGACTATGATAACCGCCCTTTCTGGTTCCGCTGCATGTACATGCTGATCTGGGGCAAATTTGTG
  CTGTACAAATACGTCACCTGCTGGCTGGTCACAGAAGGAGTGTGCATTCTGTCGGGCCTGGGCTTTAATG
  GCTTCGATGAAAATGGGACCGTGAGATGGGATGCCTGTGCCAACATGAAAGTGTGGCTCTTTGAAACCAC
  CCCTCGCTTCAATGGCACCATCGCCTCTTTCAACATCAATACCAATGCCTGGGTAGCCCGTTACATCTTC
  AAACGCCTCAAGTTCCTTGGAAATAAAGAGCTCTCACAAGGTCTCTCCTTGCTGTTCTTGGCCCTCTGGC
  ATGGCCTACACTCAGGATACCTGATTTGCTTCCAGATGGAATTCCTCATTGTTATCGTGGAAAAGCAGGT
  CAGCAGTCTAATTCGGGACAGCCCTGCCCTGAGCAGCCTGGCCTCCATCACTGCCCTACAGCCCTTCTAC
  TACTTGGTGCAACAGACCATCCACTGGCTGTTCATGGGTTACTCTATGACTGCCTTCTGCCTCTTCACAT
  GGGACAAATGGCTTAAGGTGTACAGATCCATCTATTTCCTTGGACATGTCTTCTTCTTGAGCCTACTATT
  CATATTGCCTTATATCCACAAAGCAATGGTGCCAAGAAAAGAAAAGTTAAAAAAGAGGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207801 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASTADGDMGETLEQMRGLWPGVEDLSLNKLATSLGASEQALRLIFSIFLGYPLALFYRHYLFYKDSYLI
  HLFHTFTGLSIAYFNFGHQFYHSLLCVVLQFLILRLMGRTVTAVITTLCFQMAYLLAGYYYTATGDYDIK
  WTMPHCVLTLKLIGLCIDYYDGGKDGNSLTSEQQKYAIRGVPSLLEVAGFSYFYGAFLVGPQFSMNHYMK
  LVRGQLTDIPGKMPNSTIPALKRLSLGLVYLVGYTLLSPHITDDYLLTEDYDNRPFWFRCMYMLIWGKFV
  LYKYVTCWLVTEGVCILSGLGFNGFDENGTVRWDACANMKVWLFETTPRFNGTIASFNINTNAWVARYIF
  KRLKFLGNKELSQGLSLLFLALWHGLHSGYLICFQMEFLIVIVEKQVSSLIRDSPALSSLASITALQPFY
  YLVQQTIHWLFMGYSMTAFCLFTWDKWLKVYRSIYFLGHVFFLSLLFILPYIHKAMVPRKEKLKKRE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145130
• ORF Size: 1464 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145130.2
• RefSeq Size: 2502 bp
• RefSeq ORF: 1464 bp
• Locus ID: 14792
• UniProt ID: Q91V01
• Cytogenetics: 6 59.17 cM
• MW: 56.1 kDa
• Gene Summary: Acyltransferase which mediates the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC) (LPCAT activity). To a lesser extent, also catalyzes the acylation of lysophosphatidylethanolamine (1-acyl-sn-glycero-3-phosphoethanolamine or LPE) into phosphatidylethanolamine (1,2-diacyl-sn-glycero-3-phosphoethanolamine or PE) (LPEAT activity), and the conversion of lysophosphatidylserine (1-acyl-2-hydroxy-sn-glycero-3-phospho-L-serine or LPS) into phosphatidylserine (1,2-diacyl-sn-glycero-3-phospho-L-serine or PS) (LPSAT activity). Favors polyunsaturated fatty acyl-CoAs as acyl donors compared to saturated fatty acyl-CoAs. Seems to be the major enzyme contributing to LPCAT activity in the liver. Lysophospholipid acyltransferases (LPLATs) catalyze the reacylation step of the phospholipid remodeling pathway also known as the Lands cycle.[UniProtKB/Swiss-Prot Function]