Eif2s3y (NM_012011) Mouse Tagged ORF Clone

CAT#: MR207547

Eif2s3y (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2, subunit 3, structural gene Y-linked (Eif2s3y)

Product Images

Circular map for MR207547

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eif2s3y
• Synonyms: Eif-2gy; Spy; Tfy
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207547 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGCGGAGAAGCCGGTGTCACTCTCGGGCAGCCGCATCTTTCTCGTCAGGATCTTGCCACTTTGG
  ATGTTACCAAGTTGACTCCGCTTTCACGTGAAATTATCAGCAGACAAGCCACAATTAATATAGGCACAAT
  TGGTCATGTTGCTCATGGAAAATCTACAGTTGTAAAAGCCATTTCTGGTGTTCACACTGTCCGATTCAAA
  AATGAACTAGAAAGGAATATTACCATAAAACTTGGATATGCTAATGCCAAAATTTATAAGCTTGATGACT
  CAAGTTGTCCTCGACCAGAATGTTACAGATCTTGTGGAAGTAGTACACCTGATGAGTTTCCTTCAGATAT
  TCCAGGGACCAAAGGAAACTTCAGACTAGTCAGACATGTTTCCTTTGTTGATTGTCCTGGTCATGATATT
  TTGATGGCAACTATGCTGAATGGGGCAGCAGTGATGGATGCAGCTCTTCTGTTGATAGCTGGTAATGAAT
  CTTGTCCTCAACCTCAGACTTCTGAACACCTGGCTGCCATTGAAATTATGAAGCTAAAACATATTTTGAT
  TCTGCAAAATAAAATTGATTTGGTGAAAGAAAGCCAGGCTAAAGAACAGTATGAACAGATACTTGCATTT
  GTACAGGGTACAGTAGCCGAAGGAGCTCCTATTATTCCAATTTCTGCTCAGTTAAAATACAATATTGAAG
  TTGTATGTGAGTATATAGTAAAGAAAATTCCAGTACCTCTAAGAGACTTTACTTCAGAACCCCGACTTAT
  TGTTATTCGGTCTTTTGATGTTAACAAACCTGGCTGTGAAGTTGATGACCTTAAAGGGGGTGTAGCTGGT
  GGTAGTATTTTAAAAGGCGTATTAAAGGTGGGACAAGAGATAGAAGTGAGACCTGGTATTGTTTCTAAAG
  ACGGAGAAGGGAAGCTTATGTGTAAACCAATCTTTTCCAAGATTGTATCCCTTTTTGCAGAACACAATGA
  TCTTCAGTATGCTGCTCCAGGTGGTCTTATTGGAGTTGGAACAAAAATTGACCCAACGTTATGCCGAGCA
  GATAGAATGGTTGGGCAGGTCCTTGGTGCTGTTGGAGCATTACCTGAGATATTCACAGAGTTAGAAATTT
  CCTACTTCCTACTGAGACGGCTCCTAGGTGTACGTACAGAAGGAGACAAGAAAGCAGCAAAAGTTCAAAA
  GCTATCCAAGAATGAAGTACTCATGGTGAACATAGGGTCCTTGTCTACAGGAGGCAGAGTTAGTGCAGTC
  AAGGCAGATTTGGGTAAAATTGTTCTAACCAATCCAGTATGCACAGAAGTAGGAGAAAAAATTGCTCTAA
  GCCGACGAGTTGAGAAACACTGGCGTTTAATTGGTTGGGGCCAGATAAGAAGAGGCGTGACTATCAAGCC
  AACAATAGATGATGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207547 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGGEAGVTLGQPHLSRQDLATLDVTKLTPLSREIISRQATINIGTIGHVAHGKSTVVKAISGVHTVRFK
  NELERNITIKLGYANAKIYKLDDSSCPRPECYRSCGSSTPDEFPSDIPGTKGNFRLVRHVSFVDCPGHDI
  LMATMLNGAAVMDAALLLIAGNESCPQPQTSEHLAAIEIMKLKHILILQNKIDLVKESQAKEQYEQILAF
  VQGTVAEGAPIIPISAQLKYNIEVVCEYIVKKIPVPLRDFTSEPRLIVIRSFDVNKPGCEVDDLKGGVAG
  GSILKGVLKVGQEIEVRPGIVSKDGEGKLMCKPIFSKIVSLFAEHNDLQYAAPGGLIGVGTKIDPTLCRA
  DRMVGQVLGAVGALPEIFTELEISYFLLRRLLGVRTEGDKKAAKVQKLSKNEVLMVNIGSLSTGGRVSAV
  KADLGKIVLTNPVCTEVGEKIALSRRVEKHWRLIGWGQIRRGVTIKPTIDDE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012011
• ORF Size: 1419 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012011.2
• RefSeq Size: 1801 bp
• RefSeq ORF: 1419 bp
• Locus ID: 26908
• UniProt ID: Q9Z0N2
• Cytogenetics: Ypter
• MW: 51.1 kDa
• Gene Summary: As a subunit of eukaryotic initiation factor 2 (eIF2), involved in the early steps of protein synthesis. In the presence of GTP, eIF2 forms a ternary complex with initiator tRNA Met-tRNAi and then recruits the 40S ribosomal complex, a step that determines the rate of protein translation. This step is followed by mRNA binding to form the 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex. In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B (By similarity). Along with its paralog on chromosome X, may contribute to spermatogenesis up to the round spermatid stage (PubMed:26823431).[UniProtKB/Swiss-Prot Function]