Eif2s3y (NM_012011) Mouse Untagged Clone

CAT#: MC204459

Eif2s3y (untagged) - Mouse eukaryotic translation initiation factor 2, subunit 3, structural gene Y-linked (Eif2s3y), (10ug)


  "NM_012011" in other vectors (4)

Reconstitution Protocol

USD 457.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (3)
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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Eif2s3y
Synonyms Eif-2gy; Spy; Tfy
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC043656
GCAAGATGGCGGGCGGAGAAGCCGGTGTCACTCTCGGGCAGCCGCATCTTTCTCGTCAGGATCTTGCCAC TTTGGATGTTACCAAGTTGACTCCGCTTTCACGTGAAATTATCAGCAGACAAGCCACAATTAATATAGGC ACAATTGGTCATGTTGCTCATGGAAAATCTACAGTTGTAAAAGCCATTTCTGGTGTTCACACTGTCCGAT TCAAAAATGAACTAGAAAGGAATATTACCATAAAACTTGGATATGCTAATGCCAAAATTTATAAGCTTGA TGACTCAAGTTGTCCTCGACCAGAATGTTACAGATCTTGTGGAAGTAGTACACCTGATGAGTTTCCTTCA GATATTCCAGGGACCAAAGGAAACTTCAGACTAGTCAGACATGTTTCCTTTGTTGATTGTCCTGGTCATG ATATTTTGATGGCAACTATGCTGAATGGGGCAGCAGTGATGGATGCAGCTCTTCTGTTGATAGCTGGTAA TGAATCTTGTCCTCAACCTCAGACTTCTGAACACCTGGCTGCCATTGAAATTATGAAGCTAAAACATATT TTGATTCTGCAAAATAAAATTGATTTGGTGAAAGAAAGCCAGGCTAAAGAACAGTATGAACAGATACTTG CATTTGTACAGGGTACAGTAGCCGAAGGAGCTCCTATTATTCCAATTTCTGCTCAGTTAAAATACAATAT TGAAGTTGTATGTGAGTATATAGTAAAGAAAATTCCAGTACCTCTAAGAGACTTTACTTCAGAACCCCGA CTTATTGTTATTCGGTCTTTTGATGTTAACAAACCTGGCTGTGAAGTTGATGACCTTAAAGGGGGTGTAG CTGGTGGTAGTATTTTAAAAGGCGTATTAAAGGTGGGACAAGAGATAGAAGTGAGACCTGGTATTGTTTC TAAAGACGGAGAAGGGAAGCTTATGTGTAAACCAATCTTTTCCAAGATTGTATCCCTTTTTGCAGAACAC AATGATCTTCAGTATGCTGCTCCAGGTGGTCTTATTGGAGTTGGAACAAAAATTGACCCAACGTTATGCC GAGCAGATAGAATGGTTGGGCAGGTCCTTGGTGCTGTTGGAGCATTACCTGAGATATTCACAGAGTTAGA AATTTCCTACTTCCTACTGAGACGGCTCCTAGGTGTACGTACAGAAGGAGACAAGAAAGCAGCAAAAGTT CAAAAGCTATCCAAGAATGAAGTACTCATGGTGAACATAGGGTCCTTGTCTACAGGAGGCAGAGTTAGTG CAGTCAAGGCAGATTTGGGTAAAATTGTTCTAACCAATCCAGTATGCACAGAAGTAGGAGAAAAAATTGC TCTAAGCCGACGAGTTGAGAAACACTGGCGTTTAATTGGTTGGGGCCAGATAAGAAGAGGCGTGACTATC AAGCCAACAATAGATGATGAATGAGATGATACATTTGGATGAAACCAGGAATTTTTTCATAACAGTCTTA GAGATATTTTCAAATCTAAAAAGTAGGACATCTAAAGAAGGAAATAAATTTCACAGCTTATTATTATATT AGTAAAAATTGCCAGGTTATTTTCATTTTCAGTAGAAAAAAATTGATGTCTGTAAAAAAAAATGACAAAC TTCTTGATACATTTAATCTGCAATTCAACAAATAGTATTATTGTTGTAGCTTGATAGTTAACAACTGTTG CTGTGCACCACTGAACTTACAGTAAAGAAAGTTTTTCTTATGTTTGAAGGATTGTTACTCTAATAAAAAC TTGAAGGCTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN NM_012011
Insert Size 1419 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC043656, AAH43656
RefSeq Size 1809 bp
RefSeq ORF 1419 bp
Locus ID 26908
UniProt ID Q9Z0N2
Cytogenetics Ypter
Gene Summary As a subunit of eukaryotic initiation factor 2 (eIF2), involved in the early steps of protein synthesis. In the presence of GTP, eIF2 forms a ternary complex with initiator tRNA Met-tRNAi and then recruits the 40S ribosomal complex, a step that determines the rate of protein translation. This step is followed by mRNA binding to form the 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex. In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B (By similarity). Along with its paralog on chromosome X, may contribute to spermatogenesis up to the round spermatid stage (PubMed:26823431).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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