Becn1 (NM_019584) Mouse Tagged ORF Clone

CAT#: MR207162

Becn1 (Myc-DDK-tagged) - Mouse beclin 1, autophagy related (Becn1)

Product Images

Circular map for MR207162

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Becn1
• Synonyms: 4921513J16Rik; 5430417M23Rik; Atg6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207162 representing NM_019584
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGGTCTAAGGCGTCCAGCAGCACCATGCAGGTGAGCTTCGTGTGCCAGCGCTGTAGCCAGCCTC
  TGAAACTGGACACGAGCTTCAAGATCCTGGACCGGGTCACCATCCAGGAACTCACAGCTCCATTACTTAC
  CACAGCCCAGGCGAAACCAGGAGAGACCCAGGAGGAAGAGGCTAACTCAGGAGAGGAGCCATTTATTGAA
  ACTCGCCAGGATGGTGTCTCTCGAAGATTCATCCCCCCAGCCAGGATGATGTCTACAGAAAGTGCTAATA
  GCTTCACTCTGATCGGGGAGGCATCTGATGGTGGCACCATGGAGAACCTCAGCCGGAGACTCAAGGTCAC
  TGGAGACCTGTTTGACATCATGTCGGGCCAGACCGATGTGGATCACCCGCTGTGTGAGGAATGCACAGAC
  ACTCTTTTAGACCAGCTGGACACTCAGCTCAATGTCACTGAGAATGAATGTCAGAACTACAAACGCTGTT
  TGGAGATCCTAGAGCAGATGAATGAGGATGACAGTGAGCAGCTGCAGAGGGAGCTGAAGGAGCTGGCCTT
  GGAGGAGGAGAGGCTGATCCAGGAGCTGGAAGATGTGGAAAAGAACCGCAAGGTGGTGGCAGAGAACCTG
  GAGAAGGTCCAGGCTGAGGCGGAGAGATTGGACCAGGAGGAAGCTCAGTACCAGCGGGAGTATAGTGAGT
  TTAAAAGGCAGCAGCTGGAGTTGGATGACGAACTCAAGAGTGTGGAGAACCAGGTGCGCTACGCCCAGAT
  CCAGCTGGACAAGCTCAAGAAAACCAATGTCTTCAATGCCACCTTCCACATCTGGCACAGCGGACAGTTT
  GGCACAATCAATAATTTCAGACTGGGTCGCTTGCCCAGTGTTCCTGTGGAGTGGAATGAAATCAATGCTG
  CCTGGGGCCAGACAGTGCTGCTGCTCCATGCTTTGGCCAATAAGATGGGTCTGAAGTTTCAGAGGTACCG
  ACTTGTTCCCTATGGAAATCATTCCTATCTGGAGTCTCTGACAGACAAATCTAAGGAGTTGCCGTTATAC
  TGTTCTGGGGGTTTGCGGTTTTTCTGGGACAACAAGTTTGACCATGCAATGGTAGCTTTTCTGGACTGTG
  TGCAGCAGTTCAAAGAAGAGGTGGAAAAAGGAGAGACTCGATTTTGTCTTCCGTACAGGATGGACGTGGA
  GAAAGGCAAGATTGAAGACACTGGAGGCAGTGGCGGCTCCTATTCCATCAAAACCCAGTTTAACTCGGAG
  GAGCAGTGGACAAAAGCGCTCAAGTTCATGCTGACCAATCTCAAGTGGGGTCTTGCCTGGGTGTCCTCAC
  AGTTCTATAACAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207162 representing NM_019584
  Red=Cloning site Green=Tags(s)
  
  MEGSKASSSTMQVSFVCQRCSQPLKLDTSFKILDRVTIQELTAPLLTTAQAKPGETQEEEANSGEEPFIE
  TRQDGVSRRFIPPARMMSTESANSFTLIGEASDGGTMENLSRRLKVTGDLFDIMSGQTDVDHPLCEECTD
  TLLDQLDTQLNVTENECQNYKRCLEILEQMNEDDSEQLQRELKELALEEERLIQELEDVEKNRKVVAENL
  EKVQAEAERLDQEEAQYQREYSEFKRQQLELDDELKSVENQVRYAQIQLDKLKKTNVFNATFHIWHSGQF
  GTINNFRLGRLPSVPVEWNEINAAWGQTVLLLHALANKMGLKFQRYRLVPYGNHSYLESLTDKSKELPLY
  CSGGLRFFWDNKFDHAMVAFLDCVQQFKEEVEKGETRFCLPYRMDVEKGKIEDTGGSGGSYSIKTQFNSE
  EQWTKALKFMLTNLKWGLAWVSSQFYNK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019584
• ORF Size: 1344 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019584.4
• RefSeq Size: 2031 bp
• RefSeq ORF: 1347 bp
• Locus ID: 56208
• UniProt ID: O88597
• Cytogenetics: 11 D
• MW: 52 kDa
• Gene Summary: Plays a central role in autophagy (PubMed:10604474, PubMed:12372286, PubMed:19270693, PubMed:28445460). Acts as core subunit of different PI3K complex forms that mediate formation of phosphatidylinositol 3-phosphate and are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis (PubMed:19270693, PubMed:25275521). Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (By similarity). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms (PubMed:25275521). Involved in endocytosis including endosome formation in neuronal cells (PubMed:25275521). May play a role in antiviral host defense (By similarity).[UniProtKB/Swiss-Prot Function]