Fads1 (NM_146094) Mouse Tagged ORF Clone

CAT#: MR207139

Fads1 (Myc-DDK-tagged) - Mouse fatty acid desaturase 1 (Fads1)

Product Images

Circular map for MR207139

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fads1
• Synonyms: 0710001O03Rik; A930006B21Rik; AI317215; DSD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207139 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCCGACCCGGTGCCGACCCCTGGCCCGGCCTCCGCCCAGCTCCGCCAAACGCGCTACTTTACTT
  GGGAGGAGGTGGCGCAGCGCTCCGGGCGGGAGAAGGAGCGATGGCTCGTGATCGACCGGAAGGTGTACAA
  CATCAGCGACTTCAGCCGCCGCCACCCGGGGGGCTCCCGGGTCATCAGCCACTACGCGGGTCAGGATGCC
  ACGGATCCTTTTGTGGCATTCCACATCAACAAGGGTCTTGTGAGAAAGTATATGAACTCTCTTCTGATTG
  GAGAGCTGGCTCCGGAGCAACCCAGCTTTGAACCCACCAAGAATAAAGCGCTAACTGATGAATTCCGGGA
  GCTGCGGGCCACAGTAGAGCGAATGGGCCTCATGAAGGCCAACCACCTCTTCTTCCTGGTCTACCTGCTT
  CACATCCTGCTGCTGGATGTGGCTGCCTGGCTCACCCTTTGGATCTTTGGAACTTCCTTGGTGCCCTTCA
  TCCTCTGTGCAGTACTGCTCAGTACAGTTCAGGCTCAGGCAGGTTGGCTACAGCATGACTTTGGGCACCT
  GTCAGTCTTTGGCACCTCGACATGGAATCACCTGCTACATCATTTTGTGATTGGCCACCTGAAGGGGGCC
  CCCGCCAGCTGGTGGAACCACATGCATTTCCAGCACCATGCCAAGCCTAACTGCTTCCGCAAGGACCCCG
  ATATCAACATGCACCCCCTCTTCTTCGCCCTGGGGAAGGTCCTTCCTGTGGAGCTCGGGAGGGAAAAGAA
  GAAGCACATGCCATACAACCATCAGCACAAGTACTTCTTCCTCATCGGACCCCCAGCCTTGCTGCCTCTA
  TACTTCCAGTGGTATATTTTCTATTTTGTGGTTCAGCGGAAGAAATGGCTGGACTTGGCCTGGATGCTCA
  GCTTCTATGCCCGCATCTTCTTCACTTACATGCCGCTGCTGGGGCTGAAAGGCTTCCTGGGCCTTTTCTT
  CATTGTCAGGTTCCTGGAAAGCAACTGGTTTGTGTGGGTGACACAGATGAACCATATCCCCATGCACATT
  GATCACGACCGGAATGTGGACTGGGTCTCCACCCAGCTGCAGGCAACCTGCAACATTCACCAATCAGCCT
  TCAACAACTGGTTCAGTGGCCACCTAAATTTCCAGATTGAACACCACCTCTTCCCCACCATGCCGCGGCA
  CAACTACCACAAGGTGGCACCCCTAGTACAATCCCTGTGCGCCAAGTACGGCATCAAGTATGAGTCCAAG
  CCCCTGCTCACAGCCTTCGCGGACATTGTTTACTCCCTGAAGGAGTCAGGGCAACTCTGGTTGGACGCTT
  ACCTTCACCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207139 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPDPVPTPGPASAQLRQTRYFTWEEVAQRSGREKERWLVIDRKVYNISDFSRRHPGGSRVISHYAGQDA
  TDPFVAFHINKGLVRKYMNSLLIGELAPEQPSFEPTKNKALTDEFRELRATVERMGLMKANHLFFLVYLL
  HILLLDVAAWLTLWIFGTSLVPFILCAVLLSTVQAQAGWLQHDFGHLSVFGTSTWNHLLHHFVIGHLKGA
  PASWWNHMHFQHHAKPNCFRKDPDINMHPLFFALGKVLPVELGREKKKHMPYNHQHKYFFLIGPPALLPL
  YFQWYIFYFVVQRKKWLDLAWMLSFYARIFFTYMPLLGLKGFLGLFFIVRFLESNWFVWVTQMNHIPMHI
  DHDRNVDWVSTQLQATCNIHQSAFNNWFSGHLNFQIEHHLFPTMPRHNYHKVAPLVQSLCAKYGIKYESK
  PLLTAFADIVYSLKESGQLWLDAYLHQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146094
• ORF Size: 1344 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146094.1, NM_146094.2, NP_666206.1
• RefSeq Size: 3474 bp
• RefSeq ORF: 1344 bp
• Locus ID: 76267
• UniProt ID: Q920L1
• Cytogenetics: 19 A
• MW: 52.4 kDa
• Gene Summary: Acts as a front-end fatty acyl-coenzyme A (CoA) desaturase that introduces a cis double bond at carbon 5 located between a preexisting double bond and the carboxyl end of the fatty acyl chain. Involved in biosynthesis of highly unsaturated fatty acids (HUFA) from the essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3) precursors. Specifically, desaturates dihomo-gamma-linoleoate (DGLA) (20:3n-6) and eicosatetraenoate (ETA) (20:4n-3) to generate arachidonate (AA) (20:4n-6) and eicosapentaenoate (EPA) (20:5n-3), respectively (Probable). As a rate limiting enzyme for DGLA (20:3n-6) and AA (20:4n-6)-derived eicosanoid biosynthesis, controls the metabolism of inflammatory lipids like prostaglandin E2, critical for efficient acute inflammatory response and maintenance of epithelium homeostasis. Contributes to membrane phospholipid biosynthesis by providing AA (20:4n-6) as a major acyl chain esterified into phospholipids. In particular, regulates phosphatidylinositol-4,5-bisphosphate levels, modulating inflammatory cytokine production in T-cells (PubMed:22534642). Also desaturates (11E)-octadecenoate (trans-vaccenoate)(18:1n-9), a metabolite in the biohydrogenation pathway of LA (18:2n-6) (By similarity).[UniProtKB/Swiss-Prot Function]