Fads1 (NM_146094) Mouse Untagged Clone

CAT#: MC204525

Fads1 (untagged) - Mouse fatty acid desaturase 1 (Fads1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fads1
• Synonyms: 0710001O03Rik; A930006B21Rik; AI317215; DSD
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC026848
  CGGACGCGTGGGCGACGCTCCCCTAGTTCGCGAGCCTGGCGTGCGCTGCCCCGCGGCCCGGAGCGCGCAC CTCTCAGACTCCAGCTTCCCCCGCCAAGCTTGCTATGGCTCCCGACCCGGTGCCGACCCCTGGCCCGGCC TCCGCCCAGCTCCGCCAAACGCGCTACTTTACTTGGGAGGAGGTGGCGCAGCGCTCCGGGCGGGAGAAGG AGCGATGGCTCGTGATCGACCGGAAGGTGTACAACATCAGCGACTTCAGCCGCCGCCACCCGGGGGGCTC CCGGGTCATCAGCCACTACGCGGGTCAGGATGCCACGGATCCTTTTGTGGCATTCCACATCAACAAGGGT CTTGTGAGAAAGTATATGAACTCTCTTCTGATTGGAGAGCTGGCTCCGGAGCAACCCAGCTTTGAACCCA CCAAGAATAAAGCGCTAACTGATGAATTCCGGGAGCTGCGGGCCACAGTAGAGCGAATGGGCCTCATGAA GGCCAACCACCTCTTCTTCCTGGTCTACCTGCTTCACATCCTGCTGCTGGATGTGGCTGCCTGGCTCACC CTTTGGATCTTTGGAACTTCCTTGGTGCCCTTCATCCTCTGTGCAGTACTGCTCAGTACAGTTCAGGCTC AGGCAGGTTGGCTACAGCATGACTTTGGGCACCTGTCAGTCTTTGGCACCTCGACATGGAATCACCTGCT ACATCATTTTGTGATTGGCCACCTGAAGGGGGCCCCCGCCAGCTGGTGGAACCACATGCATTTCCAGCAC CATGCCAAGCCTAACTGCTTCCGCAAGGACCCCGATATCAACATGCACCCCCTCTTCTTCGCCCTGGGGA AGGTCCTTCCTGTGGAGCTCGGGAGGGAAAAGAAGAAGCACATGCCATACAACCATCAGCACAAGTACTT CTTCCTCATCGGACCCCCAGCCTTGCTGCCTCTATACTTCCAGTGGTATATTTTCTATTTTGTGGTTCAG CGGAAGAAATGGCTGGACTTGGCCTGGATGCTCAGCTTCTATGCCCGCATCTTCTTCACTTACATGCCGC TGCTGGGGCTGAAAGGCTTCCTGGGCCTTTTCTTCATTGTCAGGTTCCTGGAAAGCAACTGGTTTGTGTG GGTGACACAGATGAACCATATCCCCATGCACATTGATCACGACCGGAATGTGGACTGGGTCTCCACCCAG CTGCAGGCAACCTGCAACATTCACCAATCAGCCTTCAACAACTGGTTCAGTGGCCACCTAAATTTCCAGA TTGAACACCACCTCTTCCCCACCATGCCGCGGCACAACTACCACAAGGTGGCACCCCTAGTACAATCCCT GTGCGCCAAGTACGGCATCAAGTATGAGTCCAAGCCCCTGCTCACAGCCTTCGCGGACATTGTTTACTCC CTGAAGGAGTCAGGGCAACTCTGGTTGGACGCTTACCTTCACCAATAGCAGCAGCAGACCTCCATATGGA GGAGGAAGAGGACACCTGGAGCCAAAGCAGGAGTTGGAGGGACAATGCCACTATGAATCTAATGTTTAAA AGGTTAGGGAACATGGATATTCGTACTCACATCTGCGCCCCTCCCCCTTGCCTTCTGGGTACCAAACTTG AGGGAGGGGAGAGCTGTTGAAGCAGGAATTCAAAACTGTTATTTCTTCTACTTTGGCAGGGGAGGGGGAC AAATGGTGGTACCTACAGAGCTCTGCATGAAGTAGGGACCCCACACTAGATAACCCCTCTTGTAAAGTAT GAGCCCATCTCACTGGGCTATCTGACTCTCCACCCTCCTTGTTAGTCTCTGAGGAACAGTGCAACTCCTT CGAACATGATTTAGAAAGAGGGCATTCATGCATAGAATTCTGAGGCCTGGCACAACTCCCTGCCCAGGAG CACAGCTTGCTGCTGAGGGCTGGGTGTGACCGTGTCTGCCTCTGGCTGCTGGGAGAAGCTGTTTTTCAGT TTCAGAAGCAGGCAGGACAAAGGCCAGGACTGTGGTTTGGGAGGCATTTGGCCCTGGAAGGTCCTGGCAC ACGCAATTCTTGGGTCTCATTCCATCCAGGTTCCAGAGAGAACATTAGATCTTAGCTCTTGACAAAACGG ATGGCAGATACTGGCCTTTGAAACTCTGTTGATTCATCTCTTCTAATCGGTCCCCAAGGAGAAAGAACAT AAACAAAGAGTGAGGTCTTCCCTCCCACTGAACACTATTCCCATAGTTTCCTTTTGCTCCCCAGCCTGTA ATTCTAAGTTCAACAAAACAGTTGTCAACAAAAGTGACTGAGGCTTCCACCAGAGTGCCAGGGCTCTGTG CAGACTCAGAATCCCTGTTCCCTATGTCACGTTCCAGCATTGTATAGCACAGTTCCATGTCACAAACAGG AAGGTTAGAAACACTAAGGTCTGTGAACATCACTGCTGCAGCGAGGCAGCTCCTCTGTCTACTCGTTCAA GTGTCTTACTCTACTGGATGCTGGATAGAAATTTAGGGTAACTTCTGACAAAGCGTGTCGGTCTAACAAT AAAATAAGGTTTTGTAATCTTCTGGTTGGTAAAAGATATGTTCCGGAGAATATAGGAACATGCCCGGAGA GACCTAACCTGTATGTCCACTTTCATGCTCTTGTTCCCCAAACCCCTGAGCATTGTTGTCTGCTCAAGAG ATTCTACTGGAAGCATGATGACCACCTGTCTGGGAAGAGAAAACCTCAAGAAGGCAAAGTGCAGTGCGTG TTTGTGGTCAAGATGAGGGTGTTAAGGGATGTTGGAGTCTGGTGTAGAGACAGGCAGGTTGCAGAAAAGC ACAGATCTTTGCCAGTGGTTGGCCCTGATTGAAAACCTGTTTGAAACAGACTGTGTAGGAAAAATCAGGA TTAAAGAGTGCTGCCTTTATGGCTCCTTCTTCAGAATAGGTGCAGACCCCTCCCCGTGCCCGTTCCCTGC CACCCGGGGTAAGGAGGTGGCACTACCAGTCTGTCTAACCCACTCACTGGCTTTGACCTTCAACTGCCAG TCTGGGCAAATACCAGTGAGCTCTGTTGTACCAAGACCAGGTCTTCAGAGCATCTGAACCACTGTGGCCT TCTCTTCTCAGCCTTCACTGTGACACGGGGGACATTGAGTGACAAGCCTCAGAATGTTACAGAGGTAAAG TGAGGCATCTCAAATCCACTGACAGATAAGAAAACCGCGTTAGAGGGAAAGTCACCTCTGCTATTGTAGA AAGTGGCAGAATATCCAAACTTAACTCTAGCTTCCATGTCTGTCTTTATTACCTTCATCTTCCTACTTCC TGTCCCACCGGCATCTCACTTAATTGAGCCTAATGTCAGTATCTGTGTGTGCCAATGCTGTTACCACATC CTGGTGAAGCTAAAAAATAAAATTTAATTTGGACCAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_146094
• Insert Size: 1344 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC026848, AAH26848
• RefSeq Size: 3416 bp
• RefSeq ORF: 1344 bp
• Locus ID: 76267
• UniProt ID: Q920L1
• Cytogenetics: 19 A
• Gene Summary: Acts as a front-end fatty acyl-coenzyme A (CoA) desaturase that introduces a cis double bond at carbon 5 located between a preexisting double bond and the carboxyl end of the fatty acyl chain. Involved in biosynthesis of highly unsaturated fatty acids (HUFA) from the essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3) precursors. Specifically, desaturates dihomo-gamma-linoleoate (DGLA) (20:3n-6) and eicosatetraenoate (ETA) (20:4n-3) to generate arachidonate (AA) (20:4n-6) and eicosapentaenoate (EPA) (20:5n-3), respectively (Probable). As a rate limiting enzyme for DGLA (20:3n-6) and AA (20:4n-6)-derived eicosanoid biosynthesis, controls the metabolism of inflammatory lipids like prostaglandin E2, critical for efficient acute inflammatory response and maintenance of epithelium homeostasis. Contributes to membrane phospholipid biosynthesis by providing AA (20:4n-6) as a major acyl chain esterified into phospholipids. In particular, regulates phosphatidylinositol-4,5-bisphosphate levels, modulating inflammatory cytokine production in T-cells (PubMed:22534642). Also desaturates (11E)-octadecenoate (trans-vaccenoate)(18:1n-9), a metabolite in the biohydrogenation pathway of LA (18:2n-6) (By similarity).[UniProtKB/Swiss-Prot Function]