Wipi1 (NM_145940) Mouse Tagged ORF Clone
CAT#: MR207122
- TrueORF®
Wipi1 (Myc-DDK-tagged) - Mouse WD repeat domain, phosphoinositide interacting 1 (Wipi1)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_145940" in other vectors (4)
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USD 198.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Wipi1 |
Synonyms | 4930533H01Rik; AW411817; D11Ertd498e |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR207122 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCCGAAGCCGCAGATGCCCCTCCGGGCCGAGTGGAGGCGGCGCTCAGCTGCTTCTCTTTCAACC AAGACTGCACATCCCTAGCGATTGGAACCAAGGCCGGTTACAAGCTGTTTTCTCTGAGTTCTGTGGAGCA GCTTGACCAAGTCCATGGAAGCAATGAAATCCCTGACGTGTATATCGTGGAGCGCCTCTTCTCCAGCAGC CTGGTTGTAGTGGTCAGTCACACAAAACCTCGGCAGATGAACGTCTACCATTTCAAGAAAGGCACTGAGA TCTGTAATTACAGCTACTCCAGCAACATTTTGTCTATTCGGCTCAACCGACAGAGGCTGCTGGTCTGCCT GGAAGAATCCATCTATATCCACAACATTAAGGATATGAAGTTATTGAAGACCGTCCTGGATATTCCCTCA AACCCAACAGGTCTCTGTGCCCTGTCTATCAACCATTCCAACTCTTACCTGGCCTATCCTGGAAGCCAGA GTACAGGCGAGATTGTACTCTATGATGGAAACTCCCTGAAAACGGTGTGCACCATTGCTGCCCACGAGGG GACGCTGGCCGCTATCACCTTCAACTCCTCGGGCTCCAAGCTAGCAAGCGCGTCTGAAAAAGGCACTGTC ATCCGAGTGTTCTCTGTTCCCGAGGGCCAGAAACTCTATGAGTTTCGTCGAGGAATGAAAAGGTATGTGA CAATCAGCTCCCTGGTGTTCAGTATGGACTCCCAGTTCCTGTGTGCCTCCAGCAACACGGAGACCGTGCA CATCTTCAAGATGGAACACCTGACAGACAGCCGCCCAGAAGAGCCTTCCACCTGGAGCGGCTACATGGGA AAGATGTTCATGGCAGCTACCAACTACCTCCCCGCCCAGGTGTCGGACATGATGAACCAGGACAGGGCTT TCGCCACAGGACGCCTGAACTTCTCTGGGCAGAAGAACATTTGCACCCTGTCCACGATCCAGAAACTGCC GCGGTTGCTGGTGGCCTCCTCCGACGGACACCTTTACATCTACAACTTGGACCCACAGGATGGAGGAGAA TGTGTCCTAATCAAAACCCACAGCTTGCTTAGCTCAGGAACAACAGAAGAGAACAAAGAAAATGACCTCA GACCTTCCTTACCTCCATCTTATGCTGCAACTGTAGCAAGGCCCAGCACGTCTGCAGCCTCCACGGTGCC AGGATACTCTGAGGACGGCGGGGCGCTCCGAGGGGAAGTTATTCCGGAACACGAGTTTGCGACGGGACCA GTGTGTCTAGACGACGAGAATGAGTTTCCCCCTATAATCTTGTGCCGTGGAAGTCAGAAGGGCAAAACGA AGCAGTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207122 protein sequence
Red=Cloning site Green=Tags(s) MEAEAADAPPGRVEAALSCFSFNQDCTSLAIGTKAGYKLFSLSSVEQLDQVHGSNEIPDVYIVERLFSSS LVVVVSHTKPRQMNVYHFKKGTEICNYSYSSNILSIRLNRQRLLVCLEESIYIHNIKDMKLLKTVLDIPS NPTGLCALSINHSNSYLAYPGSQSTGEIVLYDGNSLKTVCTIAAHEGTLAAITFNSSGSKLASASEKGTV IRVFSVPEGQKLYEFRRGMKRYVTISSLVFSMDSQFLCASSNTETVHIFKMEHLTDSRPEEPSTWSGYMG KMFMAATNYLPAQVSDMMNQDRAFATGRLNFSGQKNICTLSTIQKLPRLLVASSDGHLYIYNLDPQDGGE CVLIKTHSLLSSGTTEENKENDLRPSLPPSYAATVARPSTSAASTVPGYSEDGGALRGEVIPEHEFATGP VCLDDENEFPPIILCRGSQKGKTKQS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_145940 |
ORF Size | 1341 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_145940.2, NP_666052.1 |
RefSeq Size | 1817 bp |
RefSeq ORF | 1341 bp |
Locus ID | 52639 |
UniProt ID | Q8R3E3 |
Cytogenetics | 11 72.18 cM |
MW | 48.8 kDa |
Gene Summary | Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Plays an important role in starvation- and calcium-mediated autophagy, as well as in mitophagy (By similarity) (PubMed:22275429). Functions downstream of the ULK1 and PI3-kinases that produce phosphatidylinositol 3-phosphate (PtdIns3P) on membranes of the endoplasmic reticulum once activated. Binds phosphatidylinositol 3-phosphate (PtdIns3P), and maybe other phosphoinositides including PtdIns3,5P2 and PtdIns5P, and is recruited to phagophore assembly sites at the endoplasmic reticulum membranes. There, it assists WIPI2 in the recruitment of ATG12-ATG5-ATG16L1, a complex that directly controls the elongation of the nascent autophagosomal membrane. Involved in xenophagy of Staphylococcus aureus. Invading S.aureus cells become entrapped in autophagosome-like WIPI1 positive vesicles targeted for lysosomal degradation. Plays also a distinct role in controlling the transcription of melanogenic enzymes and melanosome maturation, a process that is distinct from starvation-induced autophagy. May also regulate the trafficking of proteins involved in the mannose-6-phosphate receptor (MPR) recycling pathway (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC215904 | Wipi1 (untagged) - Mouse WD repeat domain, phosphoinositide interacting 1 (Wipi1), (10ug) |
USD 503.00 |
|
MG207122 | Wipi1 (tGFP-tagged) - Mouse WD repeat domain, phosphoinositide interacting 1 (Wipi1) |
USD 657.00 |
|
MR207122L3 | Lenti ORF clone of Wipi1 (Myc-DDK-tagged) - Mouse WD repeat domain, phosphoinositide interacting 1 (Wipi1) |
USD 757.00 |
|
MR207122L4 | Lenti ORF clone of Wipi1 (mGFP-tagged) - Mouse WD repeat domain, phosphoinositide interacting 1 (Wipi1) |
USD 757.00 |
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