Wipi1 (NM_145940) Mouse Tagged ORF Clone

CAT#: MG207122

Wipi1 (tGFP-tagged) - Mouse WD repeat domain, phosphoinositide interacting 1 (Wipi1)

Product Images

Circular map for MG207122

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Wipi1
• Synonyms: 4930533H01Rik; AW411817; D11Ertd498e
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207122 representing NM_145940
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCGAAGCCGCAGATGCCCCTCCGGGCCGAGTGGAGGCGGCGCTCAGCTGCTTCTCTTTCAACC
  AAGACTGCACATCCCTAGCGATTGGAACCAAGGCCGGTTACAAGCTGTTTTCTCTGAGTTCTGTGGAGCA
  GCTTGACCAAGTCCATGGAAGCAATGAAATCCCTGACGTGTATATCGTGGAGCGCCTCTTCTCCAGCAGC
  CTGGTTGTAGTGGTCAGTCACACAAAACCTCGGCAGATGAACGTCTACCATTTCAAGAAAGGCACTGAGA
  TCTGTAATTACAGCTACTCCAGCAACATTTTGTCTATTCGGCTCAACCGACAGAGGCTGCTGGTCTGCCT
  GGAAGAATCCATCTATATCCACAACATTAAGGATATGAAGTTATTGAAGACCGTCCTGGATATTCCCTCA
  AACCCAACAGGTCTCTGTGCCCTGTCTATCAACCATTCCAACTCTTACCTGGCCTATCCTGGAAGCCAGA
  GTACAGGCGAGATTGTACTCTATGATGGAAACTCCCTGAAAACGGTGTGCACCATTGCTGCCCACGAGGG
  GACGCTGGCCGCTATCACCTTCAACTCCTCGGGCTCCAAGCTAGCAAGCGCGTCTGAAAAAGGCACTGTC
  ATCCGAGTGTTCTCTGTTCCCGAGGGCCAGAAACTCTATGAGTTTCGTCGAGGAATGAAAAGGTATGTGA
  CAATCAGCTCCCTGGTGTTCAGTATGGACTCCCAGTTCCTGTGTGCCTCCAGCAACACGGAGACCGTGCA
  CATCTTCAAGATGGAACACCTGACAGACAGCCGCCCAGAAGAGCCTTCCACCTGGAGCGGCTACATGGGA
  AAGATGTTCATGGCAGCTACCAACTACCTCCCCGCCCAGGTGTCGGACATGATGAACCAGGACAGGGCTT
  TCGCCACAGGACGCCTGAACTTCTCTGGGCAGAAGAACATTTGCACCCTGTCCACGATCCAGAAACTGCC
  GCGGTTGCTGGTGGCCTCCTCCGACGGACACCTTTACATCTACAACTTGGACCCACAGGATGGAGGAGAA
  TGTGTCCTAATCAAAACCCACAGCTTGCTTAGCTCAGGAACAACAGAAGAGAACAAAGAAAATGACCTCA
  GACCTTCCTTACCTCCATCTTATGCTGCAACTGTAGCAAGGCCCAGCACGTCTGCAGCCTCCACGGTGCC
  AGGATACTCTGAGGACGGCGGGGCGCTCCGAGGGGAAGTTATTCCGGAACACGAGTTTGCGACGGGACCA
  GTGTGTCTAGACGACGAGAATGAGTTTCCCCCTATAATCTTGTGCCGTGGAAGTCAGAAGGGCAAAACGA
  AGCAGTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207122 representing NM_145940
  Red=Cloning site Green=Tags(s)
  
  MEAEAADAPPGRVEAALSCFSFNQDCTSLAIGTKAGYKLFSLSSVEQLDQVHGSNEIPDVYIVERLFSSS
  LVVVVSHTKPRQMNVYHFKKGTEICNYSYSSNILSIRLNRQRLLVCLEESIYIHNIKDMKLLKTVLDIPS
  NPTGLCALSINHSNSYLAYPGSQSTGEIVLYDGNSLKTVCTIAAHEGTLAAITFNSSGSKLASASEKGTV
  IRVFSVPEGQKLYEFRRGMKRYVTISSLVFSMDSQFLCASSNTETVHIFKMEHLTDSRPEEPSTWSGYMG
  KMFMAATNYLPAQVSDMMNQDRAFATGRLNFSGQKNICTLSTIQKLPRLLVASSDGHLYIYNLDPQDGGE
  CVLIKTHSLLSSGTTEENKENDLRPSLPPSYAATVARPSTSAASTVPGYSEDGGALRGEVIPEHEFATGP
  VCLDDENEFPPIILCRGSQKGKTKQS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145940
• ORF Size: 1338 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145940.2, NP_666052.1
• RefSeq Size: 1817 bp
• RefSeq ORF: 1341 bp
• Locus ID: 52639
• UniProt ID: Q8R3E3
• Cytogenetics: 11 72.18 cM
• Gene Summary: Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Plays an important role in starvation- and calcium-mediated autophagy, as well as in mitophagy (By similarity) (PubMed:22275429). Functions downstream of the ULK1 and PI3-kinases that produce phosphatidylinositol 3-phosphate (PtdIns3P) on membranes of the endoplasmic reticulum once activated. Binds phosphatidylinositol 3-phosphate (PtdIns3P), and maybe other phosphoinositides including PtdIns3,5P2 and PtdIns5P, and is recruited to phagophore assembly sites at the endoplasmic reticulum membranes. There, it assists WIPI2 in the recruitment of ATG12-ATG5-ATG16L1, a complex that directly controls the elongation of the nascent autophagosomal membrane. Involved in xenophagy of Staphylococcus aureus. Invading S.aureus cells become entrapped in autophagosome-like WIPI1 positive vesicles targeted for lysosomal degradation. Plays also a distinct role in controlling the transcription of melanogenic enzymes and melanosome maturation, a process that is distinct from starvation-induced autophagy. May also regulate the trafficking of proteins involved in the mannose-6-phosphate receptor (MPR) recycling pathway (By similarity).[UniProtKB/Swiss-Prot Function]