Actl6b (NM_031404) Mouse Tagged ORF Clone

CAT#: MR206785

Actl6b (Myc-DDK-tagged) - Mouse actin-like 6B (Actl6b)

Product Images

Circular map for MR206785

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Actl6b
• Synonyms: Actl6; ArpNa; Baf53b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206785 representing NM_031404
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGGGGGCGTCTACGGCGGAGATGAGGTGGGGGCGCTCGTCTTTGACATTGGCTCTTTCTCAGTCC
  GAGCTGGGTACGCTGGGGAGGACTGCCCCAAGGCTGACTTCCCCACCACTGTGGGGCTGCTGGCCGCAGA
  AGAGGGGGGCGGGCTGGAGCTGGAGGGGGAGAAAGAGAAGAAAGGGAAGATTTTCCACATCGACACCAAT
  GCCCTGCACGTGCCTCGGGATGGAGCGGAGGTCATGTCGCCCCTCAAGAATGGCATGATTGAGGACTGGG
  AGTGCTTCCGAGCCATCCTGGATCATACCTACAGCAAACATGTCAAGTCCGAGCCAAACCTGCACCCAGT
  ACTCATGTCGGAGGCTCCGTGGAACACTCGGGCCAAGCGGGAGAAGCTGACGGAGCTGATGTTCGAGCAG
  TACAACATTCCTGCCTTCTTCTTATGCAAGACGGCCGTGCTCACAGCCTTTGCAAATGGACGCTCCACAG
  GCCTGGTGCTGGACAGTGGGGCCACCCACACTACAGCCATCCCAGTCCATGATGGCTATGTCCTACAGCA
  AGGCATCGTCAAGTCCCCCCTGGCAGGGGACTTCATCTCCATGCAGTGCCGGGAGCTCTTCCAGGAAATG
  GCTATTGATATCATTCCTCCTTACATGATTGCAGCCAAGGAGCCTGTACGGGAGGGAGCCCCCCCAAACT
  GGAAGAAGAAGGAGAAGCTACCCCAAGTCTCCAAGTCCTGGCATAACTACATGTGTAACGAGGTGATCCA
  AGACTTCCAGGCCTCCGTACTGCAGGTGTCTGATTCCCCTTACGATGAACAGGTAGCTGCACAAATGCCC
  ACTGTGCATTATGAAATGCCCAATGGCTACAACACAGACTACGGCGCTGAGCGACTTCGAATCCCTGAGG
  GCCTGTTTGATCCCTCTAATGTCAAGGGCCTGTCCGGGAATACCATGCTAGGAGTGGGTCACGTGGTCAC
  CACCAGCATCGGCATGTGTGACATTGACATTCGCCCGGGTCTCTATGGCAGTGTCATTGTCACTGGCGGC
  AACACTCTGCTTCAGGGGTTCACAGACAGACTTAATCGGGAGCTTTCTCAGAAGACCCCACCGAGCATGC
  GTCTTAAGCTCATCGCCAGCAACAGCACCATGGAACGCAAGTTCAGCCCCTGGATTGGAGGCTCCATCTT
  GGCCTCACTGGGCACATTCCAGCAGATGTGGATCTCCAAACAGGAATACGAGGAGGGAGGGAAGCAGTGC
  GTGGAGCGGAAGTGCCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206785 representing NM_031404
  Red=Cloning site Green=Tags(s)
  
  MSGGVYGGDEVGALVFDIGSFSVRAGYAGEDCPKADFPTTVGLLAAEEGGGLELEGEKEKKGKIFHIDTN
  ALHVPRDGAEVMSPLKNGMIEDWECFRAILDHTYSKHVKSEPNLHPVLMSEAPWNTRAKREKLTELMFEQ
  YNIPAFFLCKTAVLTAFANGRSTGLVLDSGATHTTAIPVHDGYVLQQGIVKSPLAGDFISMQCRELFQEM
  AIDIIPPYMIAAKEPVREGAPPNWKKKEKLPQVSKSWHNYMCNEVIQDFQASVLQVSDSPYDEQVAAQMP
  TVHYEMPNGYNTDYGAERLRIPEGLFDPSNVKGLSGNTMLGVGHVVTTSIGMCDIDIRPGLYGSVIVTGG
  NTLLQGFTDRLNRELSQKTPPSMRLKLIASNSTMERKFSPWIGGSILASLGTFQQMWISKQEYEEGGKQC
  VERKCP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031404
• ORF Size: 1278 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031404.5
• RefSeq Size: 1579 bp
• RefSeq ORF: 1281 bp
• Locus ID: 83766
• UniProt ID: Q99MR0
• Cytogenetics: 5 G2
• MW: 47.3 kDa
• Gene Summary: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex), as such plays a role in remodeling mononucleosomes in an ATP-dependent fashion, and is required for postmitotic neural development and dendritic outgrowth. During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. ACTL6B/BAF53B is not essential for assembly of the nBAF complex but is required for targeting the complex and CREST to the promoter of genes essential for dendritic growth.[UniProtKB/Swiss-Prot Function]