Fam53b (NM_212473) Mouse Tagged ORF Clone

CAT#: MR206723

Fam53b (Myc-DDK-tagged) - Mouse family with sequence similarity 53, member B (Fam53b)

Product Images

Circular map for MR206723

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fam53b
• Synonyms: A930008G19Rik; mKIAA0140
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206723 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGATGATCCTAAGGAGAAGCCTCATCAACCAGGGAGCTGACTCTGGAGCACACAGGACCTTCATCC
  CTGAACTGCACACGCCAAAGATGAGTCAAGGACCTACCCTCCTTTCCTGTGGAATTATGGAAAATGACAG
  ATGGCGAGACCTGGATAGAAAATGCCCTCTTCAGATTGACCAGCCAAGCGCCAGCATCTGGGAATGTCTG
  CCTGAAAAGTGTCAGGATGGCTCCCTGTGGCACCAGGAGGCAGTGACTGCCTGTGCGGTGACCAGCCTAA
  TCAAAGACCTCAACATAAATGACCACAATGGGAACCCCTCAGCTCCTCCCAGCAAACGCCAGTGCCGGTC
  ACTATCCTTCTCTGATGAGATGTCCAGTTGCCGGACGTCATGGCGGCCCTTGGGGTCCAAAGTCTGGACT
  CCCGTGGAGAAGAGACGATGCTACAGCGGAGGCAGCGTCCAGCGCTACTCCAATGGCGTCAGCCCCATGC
  AGCGGAGCTCTAGCTTCAGCCTCCCTGCCAGGGCCAATGGGCTCTCTTCACCTTGCCACCAGTCGAGCCT
  GCACCACCGCTTTGGGGGGCAACCCTGCCAAGGAGCACCAGGCTCAGCCCCCTGTGGACAGGCGGGTGAT
  AGCTGGAGCCCAGACCCACACCCAGTGGGAGGGGGCCGGCTGGACCTGCAGCGGTCCCTCTCTTGCTCGC
  ATGAGCAGTTTTCCTTCCCAGAATACTGTCCCCCCTCAGCCAACAGCACACCTGCCTCAACGCCAGAGCT
  GGCAAGACGCTCCAGTGGGCTTGCCCGCAGCCGCTCTCAGCCCTGTGTCCTTAATGACAAGAAGATCGGT
  GTGAAACGGCGGCGTCCGGATGAAGTGCAGGAGCAAAGACCTTCTCTAGACCTTGCCAAGATGGCACAGA
  ACTGTCAGACCTTCAGCAGCCTCAGTTGCCTGAACATGGGGGTGGACGACCACAGCTCCCAGAGCCCCTT
  TGCCCTCGTCAGCAGTACCCGATCCTGGACCGCATTGCTCTCAGCCTCCAGCCCAGGGGGCAGGACTCCT
  GCTGGGACCCCAGTTCCTGAGCCTGTTCCCCACTCCTTTGATGACCAGTTCACCTGCCAGGAGGACCTAT
  CCTGTGACGAGTCAGATGGCTGCAGTCTAGATGAGGATTGCTGCAGGAAGGGGGATCCGGCCACATCCTG
  GCGGGACCGGGGAGCCTGCACGAACAGCCTCTGCTCTTTGGATGGCGAGCTGGACATTGAGCAGATAGAG
  AACAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206723 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVMILRRSLINQGADSGAHRTFIPELHTPKMSQGPTLLSCGIMENDRWRDLDRKCPLQIDQPSASIWECL
  PEKCQDGSLWHQEAVTACAVTSLIKDLNINDHNGNPSAPPSKRQCRSLSFSDEMSSCRTSWRPLGSKVWT
  PVEKRRCYSGGSVQRYSNGVSPMQRSSSFSLPARANGLSSPCHQSSLHHRFGGQPCQGAPGSAPCGQAGD
  SWSPDPHPVGGGRLDLQRSLSCSHEQFSFPEYCPPSANSTPASTPELARRSSGLARSRSQPCVLNDKKIG
  VKRRRPDEVQEQRPSLDLAKMAQNCQTFSSLSCLNMGVDDHSSQSPFALVSSTRSWTALLSASSPGGRTP
  AGTPVPEPVPHSFDDQFTCQEDLSCDESDGCSLDEDCCRKGDPATSWRDRGACTNSLCSLDGELDIEQIE
  NN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_212473
• ORF Size: 1269 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_212473.1, NP_997638.1
• RefSeq Size: 5211 bp
• RefSeq ORF: 1269 bp
• Locus ID: 77938
• UniProt ID: Q8BGR5
• Cytogenetics: 7 F3
• MW: 45.9 kDa
• Gene Summary: Acts as a regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) nuclear localization.[UniProtKB/Swiss-Prot Function]