Fam53b (NM_212473) Mouse Untagged Clone

CAT#: MC207771

Fam53b (untagged) - Mouse family with sequence similarity 53, member B (Fam53b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fam53b
• Synonyms: A930008G19Rik; mKIAA0140
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207771 representing NM_212473
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGATGATCCTAAGGAGAAGCCTCATCAACCAGGGAGCTGACTCTGGAGCACACAGGACCTTCATCC
  CTGAACTGCACACGCCAAAGATGAGTCAAGGACCTACCCTCCTTTCCTGTGGAATTATGGAAAATGACAG
  ATGGCGAGACCTGGATAGAAAATGCCCTCTTCAGATTGACCAGCCAAGCGCCAGCATCTGGGAATGTCTG
  CCTGAAAAGTGTCAGGATGGCTCCCTGTGGCACCAGGAGGCAGTGACTGCCTGTGCGGTGACCAGCCTAA
  TCAAAGACCTCAACATAAATGACCACAATGGGAACCCCTCAGCTCCTCCCAGCAAACGCCAGTGCCGGTC
  ACTATCCTTCTCTGATGAGATGTCCAGTTGCCGGACGTCATGGCGGCCCTTGGGGTCCAAAGTCTGGACT
  CCCGTGGAGAAGAGACGATGCTACAGCGGAGGCAGCGTCCAGCGCTACTCCAATGGCGTCAGCCCCATGC
  AGCGGAGCTCTAGCTTCAGCCTCCCTGCCAGGGCCAATGGGCTCTCTTCACCTTGCCACCAGTCGAGCCT
  GCACCACCGCTTTGGGGGGCAACCCTGCCAAGGAGCACCAGGCTCAGCCCCCTGTGGACAGGCGGGTGAT
  AGCTGGAGCCCAGACCCACACCCAGTGGGAGGGGGCCGGCTGGACCTGCAGCGGTCCCTCTCTTGCTCGC
  ATGAGCAGTTTTCCTTCCCAGAATACTGTCCCCCCTCAGCCAACAGCACACCTGCCTCAACGCCAGAGCT
  GGCAAGACGCTCCAGTGGGCTTGCCCGCAGCCGCTCTCAGCCCTGTGTCCTTAATGACAAGAAGATCGGT
  GTGAAACGGCGGCGTCCGGATGAAGTGCAGGAGCAAAGACCTTCTCTAGACCTTGCCAAGATGGCACAGA
  ACTGTCAGACCTTCAGCAGCCTCAGTTGCCTGAACATGGGGGTGGACGACCACAGCTCCCAGAGCCCCTT
  TGCCCTCGTCAGCAGTACCCGATCCTGGACCGCATTGCTCTCAGCCTCCAGCCCAGGGGGCAGGACTCCT
  GCTGGGACCCCAGTTCCTGAGCCTGTTCCCCACTCCTTTGATGACCAGTTCACCTGCCAGGAGGACCTAT
  CCTGTGACGAGTCAGATGGCTGCAGTCTAGATGAGGATTGCTGCAGGAAGGGGGATCCGGCCACATCCTG
  GCGGGACCGGGGAGCCTGCACGAACAGCCTCTGCTCTTTGGATGGCGAGCTGGACATTGAGCAGATAGAG
  AACAACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_212473
• Insert Size: 1269 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC035523, AAH35523
• RefSeq Size: 5211 bp
• RefSeq ORF: 1269 bp
• Locus ID: 77938
• UniProt ID: Q8BGR5
• Cytogenetics: 7 F3
• Gene Summary: Acts as a regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) nuclear localization.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1 and 2 both encode the same isoform (a).