Tmem246 (NM_025944) Mouse Tagged ORF Clone

CAT#: MR206330

Tmem246 (Myc-DDK-tagged) - Mouse RIKEN cDNA 2810432L12 gene (2810432L12Rik)

Product Images

Circular map for MR206330

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tmem246
• Synonyms: 2810432L12Rik; 9330170P15Rik; AI835809
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206330 representing NM_025944
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCACGTCAACCTCTCCAGCTGCCATGCTTCTCCGGAGGCTTCGGCGACTCTCCTGGGGCAGCACAG
  CTGTGCAGCTCTTCATTCTAACCGTGGTGACATTTGGCTTACTGGCCCCGCTGGCCTGCCACCGGCTCCT
  CCACTCATATTTCTATCTGCGTCATTGGCATCTAAACCAGATGAGCCAGGACTTCCTGCAGCAGAGCCTG
  AAGGAGGGGGAAGCTGCCCTTCACTACTTTGAAGAGCTGCCCTCTGCCAATGGGTCTGTGCCCATCGTCT
  GGCAGGCCACTCCCCGCCCCTGGCTGGTGATTACCATAATCACTGTGGATAGGCAGCCTGGCTTTCATTA
  TGTCTTACAGGTAGTATCCCAGTTCCATCGGCTTCTGCAGCAGTGCGGCCCCCAGTGTGAAGGGCACCAG
  CTCTTCCTGTGCAATGTGGAACGGAGTGTGAGCCATTTTGATGCTAAGCTGCTCTCTAAGTACGTCCCTG
  TGGCCAACCGCTACGAGGGCACCGAAGACGATTACGGTGATGACCCTTCCACTAACTCATTTGAGAAAGA
  GAAGCAAGACTATGTGTATTGCCTGGAGTCATCGCTGCAAACCTACAATCCAGACTATGTCCTGATGGTG
  GAGGATGATGCTATTCCAGAAGAACAGATCTTCCCAGTATTGGAGCACCTCCTGCGTGCTCGCTTCTCTG
  AGCCACACCTCCAAGATGCCCTGTATCTAAAGCTCTATCACCCAGAGAGGCTACAGCACTACATCAACCC
  AGAGCCTATGCGGATCCTGGAGTGGGTTGGTGTGGGCATGCTGCTGGGGCCTGTGCTAACCTGGATCTAC
  ATGAGGTTTGCCTGCCGCCCAGGCTTCAGCTGGCCTGTCATGCTTTTCTTCTGTCTCTACAGCATGGGGC
  TGGTGGAACTGGTGGGCCGACACTATTTTCTGGAACTGCGGCGCCTGAGTCCCTCCTTGTACAGTGTGGT
  TCCTGCCTCTCAGTGTTGTACCCCGGCTATGCTCTTCCCTGCCCCTGCTGCCCGCAGGACCCTCACCTAC
  CTGTCCCAGGTGTACTGCCACAAGGGCTTTGGCAAAGACATGGCACTGTACTCTCTGCTGAGAGCCAAGG
  GGGAGAGGGCCTATGTGGTGGAGCCCAACCTTGTGAAACACATTGGGCTCTTCTCCAGCCTACGGTACAA
  TTTTCATCCCAGCCTACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206330 representing NM_025944
  Red=Cloning site Green=Tags(s)
  
  MTTSTSPAAMLLRRLRRLSWGSTAVQLFILTVVTFGLLAPLACHRLLHSYFYLRHWHLNQMSQDFLQQSL
  KEGEAALHYFEELPSANGSVPIVWQATPRPWLVITIITVDRQPGFHYVLQVVSQFHRLLQQCGPQCEGHQ
  LFLCNVERSVSHFDAKLLSKYVPVANRYEGTEDDYGDDPSTNSFEKEKQDYVYCLESSLQTYNPDYVLMV
  EDDAIPEEQIFPVLEHLLRARFSEPHLQDALYLKLYHPERLQHYINPEPMRILEWVGVGMLLGPVLTWIY
  MRFACRPGFSWPVMLFFCLYSMGLVELVGRHYFLELRRLSPSLYSVVPASQCCTPAMLFPAPAARRTLTY
  LSQVYCHKGFGKDMALYSLLRAKGERAYVVEPNLVKHIGLFSSLRYNFHPSLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025944
• ORF Size: 1209 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025944.4
• RefSeq Size: 2983 bp
• RefSeq ORF: 1212 bp
• Locus ID: 67063
• UniProt ID: Q91YV9
• Cytogenetics: 4 B1
• MW: 47 kDa
• Gene Summary: Golgi-resident glycosylphosphatidylinositol (GPI)-N-acetylgalactosamine transferase involved in the lipid remodeling steps of GPI-anchor maturation. Lipid remodeling steps consist in the generation of 2 saturated fatty chains at the sn-2 position of GPI-anchors proteins. Required for the initial step of GPI-GalNAc biosynthesis, transfers GalNAc to GPI in the Golgi after fatty acid remodeling by PGAP2.[UniProtKB/Swiss-Prot Function]