Tmem246 (NM_025944) Mouse Untagged Clone

CAT#: MC208066

Tmem246 (untagged) - Mouse RIKEN cDNA 2810432L12 gene (2810432L12Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tmem246
• Synonyms: 2810432L12Rik; 9330170P15Rik; AI835809
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208066 representing NM_025944
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCACGTCAACCTCTCCAGCTGCCATGCTTCTCCGGAGGCTTCGGCGACTCTCCTGGGGCAGCACAG
  CTGTGCAGCTCTTCATTCTAACCGTGGTGACATTTGGCTTACTGGCCCCGCTGGCCTGCCACCGGCTCCT
  CCACTCATATTTCTATCTGCGTCATTGGCATCTAAACCAGATGAGCCAGGACTTCCTGCAGCAGAGCCTG
  AAGGAGGGGGAAGCTGCCCTTCACTACTTTGAAGAGCTGCCCTCTGCCAATGGGTCTGTGCCCATCGTCT
  GGCAGGCCACTCCCCGCCCCTGGCTGGTGATTACCATAATCACTGTGGATAGGCAGCCTGGCTTTCATTA
  TGTCTTACAGGTAGTATCCCAGTTCCATCGGCTTCTGCAGCAGTGCGGCCCCCAGTGTGAAGGGCACCAG
  CTCTTCCTGTGCAATGTGGAACGGAGTGTGAGCCATTTTGATGCTAAGCTGCTCTCTAAGTACGTCCCTG
  TGGCCAACCGCTACGAGGGCACCGAAGACGATTACGGTGATGACCCTTCCACTAACTCATTTGAGAAAGA
  GAAGCAAGACTATGTGTATTGCCTGGAGTCATCGCTGCAAACCTACAATCCAGACTATGTCCTGATGGTG
  GAGGATGATGCTATTCCAGAAGAACAGATCTTCCCAGTATTGGAGCACCTCCTGCGTGCTCGCTTCTCTG
  AGCCACACCTCCAAGATGCCCTGTATCTAAAGCTCTATCACCCAGAGAGGCTACAGCACTACATCAACCC
  AGAGCCTATGCGGATCCTGGAGTGGGTTGGTGTGGGCATGCTGCTGGGGCCTGTGCTAACCTGGATCTAC
  ATGAGGTTTGCCTGCCGCCCAGGCTTCAGCTGGCCTGTCATGCTTTTCTTCTGTCTCTACAGCATGGGGC
  TGGTGGAACTGGTGGGCCGACACTATTTTCTGGAACTGCGGCGCCTGAGTCCCTCCTTGTACAGTGTGGT
  TCCTGCCTCTCAGTGTTGTACCCCGGCTATGCTCTTCCCTGCCCCTGCTGCCCGCAGGACCCTCACCTAC
  CTGTCCCAGGTGTACTGCCACAAGGGCTTTGGCAAAGACATGGCACTGTACTCTCTGCTGAGAGCCAAGG
  GGGAGAGGGCCTATGTGGTGGAGCCCAACCTTGTGAAACACATTGGGCTCTTCTCCAGCCTACGGTACAA
  TTTTCATCCCAGCCTACTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_025944
• Insert Size: 1212 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025944.3, NP_080220.2
• RefSeq Size: 2983 bp
• RefSeq ORF: 1212 bp
• Locus ID: 67063
• UniProt ID: Q91YV9
• Cytogenetics: 4 B1
• Gene Summary: Golgi-resident glycosylphosphatidylinositol (GPI)-N-acetylgalactosamine transferase involved in the lipid remodeling steps of GPI-anchor maturation. Lipid remodeling steps consist in the generation of 2 saturated fatty chains at the sn-2 position of GPI-anchors proteins. Required for the initial step of GPI-GalNAc biosynthesis, transfers GalNAc to GPI in the Golgi after fatty acid remodeling by PGAP2.[UniProtKB/Swiss-Prot Function]