Cth (NM_145953) Mouse Tagged ORF Clone

CAT#: MR206244

Cth (Myc-DDK-tagged) - Mouse cystathionase (cystathionine gamma-lyase) (Cth)

Product Images

Circular map for MR206244

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cth
• Synonyms: 0610010I13Rik; AI314617; BC019483; CSE; Cys3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206244 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAAGGACGCCTCTTTGAGCGGCTTCCTGCCTAGTTTCCAGCATTTCGCCACTCAGGCCATCCACG
  TGGGACAAGAGCCTGAGCAATGGAATTCTCGTGCCGTGGTGCTGCCCATTTCGTTGGCCACCACATTTAA
  GCAGGACTTCCCGGGCCAGTCCTCGGGTTTTGAATACAGCCGCTCTGGAAATCCAACAAGGAATTGCTTG
  GAAAAAGCAGTGGCTGCGTTGGATGGGGCAAAGCACAGTTTGGCCTTTGCATCGGGTCTTGCTGCCACCA
  TTACGATTACCCATCTTTTAAAAGCAGGAGATGAAATCATTTGCATGGATGAAGTGTATGGAGGCACCAA
  CAGGTACTTCAGGAGGGTGGCATCTGAATTTGGACTGAAGATTTCTTTTGTAGATTGTTCCAAAACCAAA
  TTGCTAGAGGCAGCGATTACACCACAAACCAAGCTTGTTTGGATCGAAACACCCACAAACCCAACTTTGA
  AGTTGGCTGACATTGGAGCCTGCGCACAAATTGTCCACAAACGTGGAGACATCATTTTGGTTGTAGATAA
  CACCTTCATGTCTGCATATTTCCAGAGACCTTTGGCTCTGGGTGCTGATATTTGTATGTGTTCTGCCACA
  AAATACATGAATGGCCACAGCGATGTTGTCATGGGTTTAGTGTCTGTTAATTCTGATGACCTCAATAGTC
  GGCTTCGTTTCCTGCAGAATTCACTAGGAGCAGTTCCTTCTCCTTTTGATTGTTACCTCTGCTGCCGAGG
  CCTGAAGACACTGCAGGTCCGGATGGAGAAACATTTCAAGAATGGGATGGCGGTGGCTCGTTTCCTGGAG
  ACCAATCCCCGGGTAGAAAAGGTTGTTTATCCTGGGCTACCCTCTCACCCTCAGCATGAGCTGGCCAAAC
  GCCAGTGCTCGGGCTGCCCAGGGATGGTCAGTTTCTACATCAAGGGTGCTCTGCAGCATGCTAAGGCCTT
  CCTCAAAAATCTAAAGCTGTTTACTCTGGCAGAGAGCCTGGGAGGATATGAGAGTCTGGCTGAGCTTCCA
  GCAATCATGACCCATGCCTCTGTGCCTGAGAAGGACAGAGCTACCCTCGGGATCAATGACACACTGATAC
  GACTTTCTGTGGGCCTAGAGGATGAACAGGACCTTCTTGAAGACCTGGATCGAGCTTTGAAGGCAGCGCA
  CCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206244 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQKDASLSGFLPSFQHFATQAIHVGQEPEQWNSRAVVLPISLATTFKQDFPGQSSGFEYSRSGNPTRNCL
  EKAVAALDGAKHSLAFASGLAATITITHLLKAGDEIICMDEVYGGTNRYFRRVASEFGLKISFVDCSKTK
  LLEAAITPQTKLVWIETPTNPTLKLADIGACAQIVHKRGDIILVVDNTFMSAYFQRPLALGADICMCSAT
  KYMNGHSDVVMGLVSVNSDDLNSRLRFLQNSLGAVPSPFDCYLCCRGLKTLQVRMEKHFKNGMAVARFLE
  TNPRVEKVVYPGLPSHPQHELAKRQCSGCPGMVSFYIKGALQHAKAFLKNLKLFTLAESLGGYESLAELP
  AIMTHASVPEKDRATLGINDTLIRLSVGLEDEQDLLEDLDRALKAAHP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145953
• ORF Size: 1197 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145953.2, NP_666065.1
• RefSeq Size: 1815 bp
• RefSeq ORF: 1197 bp
• Locus ID: 107869
• UniProt ID: Q8VCN5
• Cytogenetics: 3 H4
• MW: 43.6 kDa
• Gene Summary: Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function.[UniProtKB/Swiss-Prot Function]