Sav1 (NM_022028) Mouse Tagged ORF Clone

CAT#: MR206033

Sav1 (Myc-DDK-tagged) - Mouse salvador homolog 1 (Drosophila) (Sav1)

Product Images

Circular map for MR206033

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sav1
• Synonyms: 1700040G09Rik; Salv; Sav; WW45; Wwp3; Wwp4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206033 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTCCCGCAAGAAAACCAAAAACGAGGTGTCTAAGCCGGCCGAGGTGCAGGGCAAGTACGTGAAGA
  AGGAGACGTCGCCCCTGCTGCGGAATCTCATGCCTTCATTCATTCGGCACGGTCCAACAATTCCCAGACG
  GACTGACCTCTGTCTTCCAGATTCAAGTGCTACTGCTTTCTCAGCTTCTGGAGACGGTATAGTTTCAAGA
  AACCAGAGTTTCCTGAGAACTGCAATTCAAAGGACACCTCATGAAGTAATGAGAAGAGAAAGCCACAGAC
  TGTCTGCCCCTTCTTACCTTGTCAGGAGCCTAGCAGATGTCCCTCGAGAGTGTGGCTCATCACAGTCATT
  TTTGACAGAAGTTAACTTTGCTGTTGAGAATGGAGACTCTGGCTCCCGATACTTCTTCTCAGATAACTTT
  TTTGATGGACAGAGAAGGCGGCCACTTGGAGATCGTGCACAAGAAGATTACAGATATTATGAATACAACC
  ATGATCTCTTCCAGAGGATGCCACAGAGTCAGGGGAGGCACACTTCAGGTATTGGGAGAGTCACGGCTAC
  ATCTCTAGGGAATTTAACTAACCATGGATCTGAAGATTTACCCCTTCCTCCTGGCTGGTCTGTGGACTGG
  ACAATGAGAGGGAGAAAATACTACATAGATCATAACACAAATACCACTCACTGGAGTCATCCCCTTGAAC
  GAGAAGGACTTCCTCCTGGCTGGGAACGAGTAGAGTCATCAGAATTTGGAACCTATTACGTGGATCACAC
  CAATAAAAGGGCTCAGTACAGGCACCCCTGTGCTCCGAGTGTACCTCGGTATGATCAGCCTCCACCCATC
  ACGTATCAGCCACAACAAACTGAAAGAAATCAGTCTCTCCTGGTCCCTGCAAATCCCTACCATACTGCAG
  AAATTCCTGACTGGCTTCAGGTTTATGCCCGAGCCCCTGTGAAATATGACCACATTCTGAAGTGGGAGCT
  CTTCCAGCTGGCTGACCTGGACACGTACCAGGGAATGCTGAAGTTGCTCTTCATGAAGGAACTGGAGCAG
  ATTGTGAAGTTGTACGAGGCCTACAGACAGGCTCTTCTCACTGAGTTGGAAAACCGCAAGCAGAGGCAGC
  AGTGGTATGCCCAGCAGCATGGCAAGACGTTCTTAAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206033 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLSRKKTKNEVSKPAEVQGKYVKKETSPLLRNLMPSFIRHGPTIPRRTDLCLPDSSATAFSASGDGIVSR
  NQSFLRTAIQRTPHEVMRRESHRLSAPSYLVRSLADVPRECGSSQSFLTEVNFAVENGDSGSRYFFSDNF
  FDGQRRRPLGDRAQEDYRYYEYNHDLFQRMPQSQGRHTSGIGRVTATSLGNLTNHGSEDLPLPPGWSVDW
  TMRGRKYYIDHNTNTTHWSHPLEREGLPPGWERVESSEFGTYYVDHTNKRAQYRHPCAPSVPRYDQPPPI
  TYQPQQTERNQSLLVPANPYHTAEIPDWLQVYARAPVKYDHILKWELFQLADLDTYQGMLKLLFMKELEQ
  IVKLYEAYRQALLTELENRKQRQQWYAQQHGKTFLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022028
• ORF Size: 1161 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022028.1, NM_022028.2, NP_071311.1
• RefSeq Size: 2524 bp
• RefSeq ORF: 1161 bp
• Locus ID: 64010
• UniProt ID: Q8VEB2
• Cytogenetics: 12 C2
• MW: 44.9 kDa
• Gene Summary: Regulator of STK3/MST2 and STK4/MST1 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. SAV1 is required for STK3/MST2 and STK4/MST1 activation and promotes cell-cycle exit and terminal differentiation in developing epithelial tissues. Plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosomes, and its ability to phosphorylate CROCC and CEP250. In conjunction with STK3/MST2, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation (By similarity).[UniProtKB/Swiss-Prot Function]