Sav1 (NM_022028) Mouse Untagged Clone

CAT#: MC202958

Sav1 (untagged) - Mouse salvador homolog 1 (Drosophila) (Sav1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sav1
• Synonyms: 1700040G09Rik; Salv; Sav; WW45; Wwp3; Wwp4
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC019377 sequence for NM_022028
  GCAGCCGCCCGCGCCCAGTGAGGATATCTGGAAGAAATTCGAGCTGCTTCCCACCCCGCCCCTGTCCCCG AGGCGAAGGCGGCGGCGGCGGCCCGGGAGGCGGCGGCGGGCGGCAGGCTCGCGGCCCTAGCGAGGCTGCT GGGGCGGCGGCGGGAGTCGTCCGCGAGGATGCTGTCCCGCAAGAAAACCAAAAACGAGGTGTCTAAGCCG GCCGAGGTGCAGGGCAAGTACGTGAAGAAGGAGACGTCGCCCCTGCTGCGGAATCTCATGCCTTCATTCA TTCGGCACGGTCCAACAATTCCCAGACGGACTGACCTCTGTCTTCCAGATTCAAGTGCTACTGCTTTCTC AGCTTCTGGAGACGGTATAGTTTCAAGAAACCAGAGTTTCCTGAGAACTGCAATTCAAAGGACACCTCAT GAAGTAATGAGAAGAGAAAGCCACAGACTGTCTGCCCCTTCTTACCTTGTCAGGAGCCTAGCAGATGTCC CTCGAGAGTGTGGCTCATCACAGTCATTTTTGACAGAAGTTAACTTTGCTGTTGAGAATGGAGACTCTGG CTCCCGATACTTCTTCTCAGATAACTTTTTTGATGGACAGAGAAGGCGGCCACTTGGAGATCGTGCACAA GAAGATTACAGATATTATGAATACAACCATGATCTCTTCCAGAGGATGCCACAGAGTCAGGGGAGGCACA CTTCAGGTATTGGGAGAGTCACGGCTACATCTCTAGGGAATTTAACTAACCATGGATCTGAAGATTTACC CCTTCCTCCTGGCTGGTCTGTGGACTGGACAATGAGAGGGAGAAAATACTACATAGATCATAACACAAAT ACCACTCACTGGAGTCATCCCCTTGAACGAGAAGGACTTCCTCCTGGCTGGGAACGAGTAGAGTCATCAG AATTTGGAACCTATTACGTGGATCACACCAATAAAAGGGCTCAGTACAGGCACCCCTGTGCTCCGAGTGT ACCTCGGTATGATCAGCCTCCACCCATCACGTATCAGCCACAACAAACTGAAAGAAATCAGTCTCTCCTG GTCCCTGCAAATCCCTACCATACTGCAGAAATTCCTGACTGGCTTCAGGTTTATGCCCGAGCCCCTGTGA AATATGACCACATTCTGAAGTGGGAGCTCTTCCAGCTGGCTGACCTGGACACGTACCAGGGAATGCTGAA GTTGCTCTTCATGAAGGAACTGGAGCAGATTGTGAAGTTGTACGAGGCCTACAGACAGGCTCTTCTCACT GAGTTGGAAAACCGCAAGCAGAGGCAGCAGTGGTATGCCCAGCAGCATGGCAAGACGTTCTTAAGTTAAC TTATCACAAGCCAAGTTTATAAGAGCTTTAAAATATTTTCAGATAAATGATTGCAAACAAGTTCTTTGGT TAATAAAGGTAACTTACTATTTGGAAGTATAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_022028
• Insert Size: 1161 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC019377, AAH19377
• RefSeq Size: 1452 bp
• RefSeq ORF: 1161 bp
• Locus ID: 64010
• UniProt ID: Q8VEB2
• Cytogenetics: 12 C2
• Gene Summary: Regulator of STK3/MST2 and STK4/MST1 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. SAV1 is required for STK3/MST2 and STK4/MST1 activation and promotes cell-cycle exit and terminal differentiation in developing epithelial tissues. Plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosomes, and its ability to phosphorylate CROCC and CEP250. In conjunction with STK3/MST2, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation (By similarity).[UniProtKB/Swiss-Prot Function]