Agpat3 (NM_053014) Mouse Tagged Lenti ORF Clone
CAT#: MR205835L4
- LentiORF®
Lenti ORF clone of Agpat3 (mGFP-tagged) - Mouse 1-acylglycerol-3-phosphate O-acyltransferase 3 (Agpat3)
"NM_053014" in other vectors (4)
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USD 365.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | mGFP |
Symbol | Agpat3 |
Synonyms | AW061257; AW493985; D10Jhu12e; lpaat3 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR205835).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_053014 |
ORF Size | 1128 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_053014.3, NP_443747.2 |
RefSeq Size | 3439 bp |
RefSeq ORF | 1131 bp |
Locus ID | 28169 |
UniProt ID | Q9D517 |
Cytogenetics | 10 39.72 cM |
Gene Summary | Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (PubMed:15367102). Acts on LPA containing saturated or unsaturated fatty acids C16:0-C20:4 at the sn-1 position using C18:1, C20:4 or C18:2-CoA as the acyl donor (By similarity). Also acts on lysophosphatidylcholine, lysophosphatidylinositol and lysophosphatidylserine using C18:1 or C20:4-CoA (By similarity). Has a preference for arachidonoyl-CoA as a donor (PubMed:19114731). Has also a modest lysophosphatidylinositol acyltransferase (LPIAT) activity, converts lysophosphatidylinositol (LPI) into phosphatidylinositol (PubMed:19114731).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209809 | Agpat3 (untagged) - Mouse 1-acylglycerol-3-phosphate O-acyltransferase 3 (Agpat3), (10ug) |
USD 503.00 |
|
MG205835 | Agpat3 (tGFP-tagged) - Mouse 1-acylglycerol-3-phosphate O-acyltransferase 3 (Agpat3) |
USD 657.00 |
|
MR205835 | Agpat3 (Myc-DDK-tagged) - Mouse 1-acylglycerol-3-phosphate O-acyltransferase 3 (Agpat3) |
USD 457.00 |
|
MR205835L3 | Lenti ORF clone of Agpat3 (Myc-DDK-tagged) - Mouse 1-acylglycerol-3-phosphate O-acyltransferase 3 (Agpat3) |
USD 757.00 |
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