Agpat3 (NM_053014) Mouse Untagged Clone

CAT#: MC209809

Agpat3 (untagged) - Mouse 1-acylglycerol-3-phosphate O-acyltransferase 3 (Agpat3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Agpat3
• Synonyms: AW061257; AW493985; D10Jhu12e; lpaat3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209809 representing NM_053014
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCTGCTTGCCTACCTGAAGACCCAGTTCGTGGTGCACCTGCTCATTGGCTTCGTCTTCGTGGTGA
  GCGGGTTAATTATCAACTTCACCCAGCTGTGCACACTGGCCCTCTGGCCCATCAGCAAGCACCTATACCG
  CCGTATCAACTGCCGCTTGGCCTACTCGCTCTGGAGCCAGCTGGTCATGCTCCTGGAGTGGTGGTCCTGC
  ACGGAGTGCACTCTCTTCACCGACCAGGCCACCGTGGACCACTTTGGGAAGGAGCATGTGGTTGTTATCC
  TCAACCACAACTTCGAGATCGACTTCCTTTGTGGGTGGACGATGTGCGAGCGGTTTGGCGTGCTGGGGAG
  CTCCAAGGTGCTGGCTAAGAGGGAGCTGCTGTGTGTGCCTCTCATCGGCTGGACGTGGTACTTCCTAGAG
  ATCGTATTCTGCAAACGGAAGTGGGAGGAAGACCGGGACACCGTCATAGAGGGCCTGAGGCGCTTAGCTG
  ACTACCCAGAGTACATGTGGTTTCTCCTGTACTGCGAAGGAACACGCTTCACAGAGACCAAGCATCGCAT
  CAGCATGGAGGTGGCTGCCTCCAAGGGGCTGCCCCCACTCAAGTACCACCTGCTGCCCCGGACCAAGGGC
  TTTACCACGGCAGTCCAGTGCCTCCGGGGGACAGTTGCAGCTATCTATGACGTGACCCTGAACTTCAGAG
  GGAACAAGAACCCATCTCTACTGGGGATCCTGTATGGGAAGAAATATGAGGCAGACATGTGTGTGAGGAG
  GTTCCCCCTGGAAGACATCCCAGCAGATGAGACCAGCGCCGCCCAGTGGCTTCACAAGCTGTACCAGGAG
  AAGGATGCCCTGCAAGAGATGTACAAGCAGAAGGGTGTATTCCCAGGGGAGCAGTTCAAGCCTGCCCGAA
  GGCCGTGGACCCTCCTGAACTTCCTGTGCTGGGCCACCATCCTCCTCTCACCCCTCTTCAGCTTCGTCCT
  GGGTGTCTTTGCCAGTGGATCCCCGCTCCTCATCCTGACGTTCTTGGGGTTCGTGGGAGCAGCTTCCTTC
  GGAGTCCGGAGACTGATAGGAGTGACTGAGATAGAGAAAGGCTCCAGCTATGGCAACCAAGAGCTTAAGA
  AAAAGGAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_053014
• Insert Size: 1131 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053014.3, NP_443747.2
• RefSeq Size: 3439 bp
• RefSeq ORF: 1131 bp
• Locus ID: 28169
• UniProt ID: Q9D517
• Cytogenetics: 10 39.72 cM
• Gene Summary: Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (PubMed:15367102). Acts on LPA containing saturated or unsaturated fatty acids C16:0-C20:4 at the sn-1 position using C18:1, C20:4 or C18:2-CoA as the acyl donor (By similarity). Also acts on lysophosphatidylcholine, lysophosphatidylinositol and lysophosphatidylserine using C18:1 or C20:4-CoA (By similarity). Has a preference for arachidonoyl-CoA as a donor (PubMed:19114731). Has also a modest lysophosphatidylinositol acyltransferase (LPIAT) activity, converts lysophosphatidylinositol (LPI) into phosphatidylinositol (PubMed:19114731).[UniProtKB/Swiss-Prot Function]