Homer3 (NM_011984) Mouse Tagged ORF Clone

CAT#: MR205426

Homer3 (Myc-DDK-tagged) - Mouse homer homolog 3 (Drosophila) (Homer3), transcript variant 2

Product Images

Circular map for MR205426

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Homer3
• Synonyms: AW146114
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205426 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCACAGCCAGGGAACAGCCAATCTTCAGCACCCGGGCGCACGTATTCCAGATCGACCCCACTACAA
  AGCGGAACTGGATCCCCGCCGGCAAGCACGCACTTACCGTGTCCTATTTCTATGATGCAACCCGAAATGT
  GTACCGCATCATCAGCATCGGGGGTGCCAAGGCCATCATCAACAGCACTGTCACTCCCAACATGACCTTC
  ACCAAAACCTCTCAGAAGTTCGGGCAATGGGCAGACAGTCGAGCCAACACTGTCTACGGCCTAGGCTTTG
  CCTCTGAACAGCAGCTGACCCAGTTTGCTGAGAAGTTTCAGGAGGTGAAAGAAGCTGCCAGGCTGGCTCG
  AGAGAAATCTCAAGATGGTGGAGAATTCACTAGTACTGGCCTGGCCCTTGCCTCCCATCAGGTTCCTCCA
  AGCCCCTTGGTCAGCACCAATGGTCCAGGCGAGGAAAAGCTGTTCCGTAGCCAGAGTGCGGACACCCCTG
  GCCCCACCGAGCGGGAACGGTTGAAGAAGATGCTGTCAGAAGGCTCTGTAGGGGAGGTCCAGTGGGAGGC
  AGAGTTCTTCGCGCTTCAGGACAGCAACCAGAGGTTGGCGGGAGCCCTTCGGGAGGCCAACGCAGCGGCC
  ACTCAGTGGAGGCAACAACTGGAGGTCCAACGTGCAGAGGCTGAACTCTTGCGGCAGCGGGTAGCAGAGC
  TGGAGGCCCAGGTGGCTGTAGAGCCAGTCCGGGCAGGAGAGAAAGAAGCAACCAGCCAGTCGGTGGAGCA
  GCTGGAGGCTCGGGTGCAGACCAAGGACCAGACTTTGAAGAATCAGAGCACTGGCACCCGAGAGGCTCCA
  GACACTGCCGAGCGCGAAGAGACACAGCAGCAAGTTCAGGACCTGGAGACCCGGAATGCAGAGCTGGAGC
  AGCAGCTGCGGGCGATGGAGTGCAACCTGGAGGAGGCGCGGGCCGAGCGGGAGCGCGCACGGGCGGAGGT
  GGGCCGGGCTGCGCAGCTGCTGGATGTTCGGCTGTTTGAGCTCAGCGAGCTGCGTGAAGGCCTGGCACGC
  CTGGCAGAGGCAGCACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205426 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSTAREQPIFSTRAHVFQIDPTTKRNWIPAGKHALTVSYFYDATRNVYRIISIGGAKAIINSTVTPNMTF
  TKTSQKFGQWADSRANTVYGLGFASEQQLTQFAEKFQEVKEAARLAREKSQDGGEFTSTGLALASHQVPP
  SPLVSTNGPGEEKLFRSQSADTPGPTERERLKKMLSEGSVGEVQWEAEFFALQDSNQRLAGALREANAAA
  TQWRQQLEVQRAEAELLRQRVAELEAQVAVEPVRAGEKEATSQSVEQLEARVQTKDQTLKNQSTGTREAP
  DTAEREETQQQVQDLETRNAELEQQLRAMECNLEEARAERERARAEVGRAAQLLDVRLFELSELREGLAR
  LAEAAP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011984
• ORF Size: 1071 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011984.1
• RefSeq Size: 2436 bp
• RefSeq ORF: 1071 bp
• Locus ID: 26558
• UniProt ID: Q99JP6
• Cytogenetics: 8 B3.3
• MW: 39.7 kDa
• Gene Summary: Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. Isoforms can be differently regulated and may play an important role in maintaining the plasticity at glutamatergic synapses (By similarity). Negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway. Acts by competing with calcineurin/PPP3CA for NFAT protein binding, hence preventing NFAT activation by PPP3CA (PubMed:18218901).[UniProtKB/Swiss-Prot Function]