Homer3 (NM_011984) Mouse Untagged Clone

CAT#: MC200760

Homer3 (untagged) - Mouse homer homolog 3 (Drosophila) (Homer3), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Homer3
• Synonyms: AW146114
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC005773 sequence for NM_011984
  GCGGGGCGCGCGGAGGGCGCGGGCCGGAGGCTGCAGCGACGACGACGCGGGGGGACAAACAAGTGACTCC TGTCACCCCTCTGGGTGCCCCTTAGAGGCCATCCAGCCCAACCTGACCAATGTCCACAGCCAGGGAACAG CCAATCTTCAGCACCCGGGCGCACGTATTCCAGATCGACCCCACTACAAAGCGGAACTGGATCCCCGCCG GCAAGCACGCACTTACCGTGTCCTATTTCTATGATGCAACCCGAAATGTGTACCGCATCATCAGCATCGG GGGTGCCAAGGCCATCATCAACAGCACTGTCACTCCCAACATGACCTTCACCAAAACCTCTCAGAAGTTC GGGCAATGGGCAGACAGTCGAGCCAACACTGTCTACGGCCTAGGCTTTGCCTCTGAACAGCAGCTGACCC AGTTTGCTGAGAAGTTTCAGGAGGTGAAAGAAGCTGCCAGGCTGGCTCGAGAGAAATCTCAAGATGGTGG AGAATTCACTAGTACTGGCCTGGCCCTTGCCTCCCATCAGGTTCCTCCAAGCCCCTTGGTCAGCACCAAT GGTCCAGGCGAGGAAAAGCTGTTCCGTAGCCAGAGTGCGGACACCCCTGGCCCCACCGAGCGGGAACGGT TGAAGAAGATGCTGTCAGAAGGCTCTGTAGGGGAGGTCCAGTGGGAGGCAGAGTTCTTCGCGCTTCAGGA CAGCAACCAGAGGTTGGCGGGAGCCCTTCGGGAGGCCAACGCAGCGGCCACTCAGTGGAGGCAACAACTG GAGGTCCAACGTGCAGAGGCTGAACTCTTGCGGCAGCGGGTAGCAGAGCTGGAGGCCCAGGTGGCTGTAG AGCCAGTCCGGGCAGGAGAGAAAGAAGCAACCAGCCAGTCGGTGGAGCAGCTGGAGGCTCGGGTGCAGAC CAAGGACCAGACTTTGAAGAATCAGAGCACTGGCACCCGAGAGGCTCCAGACACTGCCGAGCGCGAAGAG ACACAGCAGCAAGTTCAGGACCTGGAGACCCGGAATGCAGAGCTGGAGCAGCAGCTGCGGGCGATGGAGT GCAACCTGGAGGAGGCGCGGGCCGAGCGGGAGCGCGCACGGGCGGAGGTGGGCCGGGCTGCGCAGCTGCT GGATGTTCGGCTGTTTGAGCTCAGCGAGCTGCGTGAAGGCCTGGCACGCCTGGCAGAGGCAGCACCCTAG TCTGCCATGGAGTGTCTGCGGCCTCAAGGCGCCCTGGCAGGGGCCAGGGGACCCCAGCTGTCTCTGAGCT TTGCACTGTGTAGAGTTTTCTAGAATCCTTGGGCAGTGCTTCTACCCAGGTTACATTTCTACGTGTGGCG TTGCTGTCCCTGGCTGCTGCTGCCCTGCGCCCCAGGGACACTGCGAGGGAAGGCTGCACTAGTCATCCCC ATGGGGCAACAGAGGCTTTGGGATCCTGAGACCTGAAGGCCCTGTACTCATCCCACCCCATTCTCAAGTC AGACTGACAACTTCAAAGAGTGTTTACTGAAGTCAGGGGCCACCAGCACCAGGTTTACAGCTCAGTCCTG AGCCTCAGCCTGGGCTGGCTCTTGGGGCCGAGATCTGGGAGGACGCGACCGTCGGACAGTGCTCCCTGCT TTCTGCCGCCGAAGTGTCTGCCCCACTTTCTCCTTGAAGCGTCGGTTTTGTTGCTTGATCTTGGCCAGCT CAGCTTTGCGTTTGGCCTCCAGGTCTGGGTCCTGCGGAAGGGAGCTGAGAATGTAACTGGGCAGCTTCCC AGGGACTGGCTCCCCCACCCCTACCCGTCCCCAGGTCCCACCCACCCTTACTGGCCACACTCTTATGCCT GTCCCTGCATACCCATGCCTCCCTATACTACCTTCCCCTCCAGGATCATCTGTTTCCGCTTGTTGATCTC TTTCTTTTCATCAAAATGCGAAGCCTCCAGTTTCTAGGGGTGGGGAGGGGAACAGGTCAGTCAGGCCTGG GGCAGGAAGCCCCGCCCACCTCACCCCACTCCACCCTACCCTGACAGGCTGGCCACACTTACTATTTCGC ACTCCCTTCGCACTACGTTGACCTGCGTGAGGATTTGTAGAACCTCAGCCTCCTCCACCACCAGCTCTGC CAGCTGCTGCTCTGCAGGGACAGGAAACACTGAGTTGGGCTGGGAGTGCAACCAGCCCTCTGCACCCCCA GCTCTGGATGTCTGGATCCAACCAAATGTGGACTGATGATATTTAGAAAAAGCAAAATGCTGCCAAGCTT GGCAGCACATGCTTGTCATCACAGCACTGGGAGGTGGAGGCAGGGGGATCACTCGTTTCAGCTGAGTTCC AGGCCAGCTCTGTAGAGCAAGAATCTGTCTCAAATTAATGACTGAATAAACAAATGAACAAGTAAAAAAA AAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_011984
• Insert Size: 1071 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC005773, AAH05773
• RefSeq Size: 2393 bp
• RefSeq ORF: 1071 bp
• Locus ID: 26558
• UniProt ID: Q99JP6
• Cytogenetics: 8 B3.3
• Gene Summary: Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. Isoforms can be differently regulated and may play an important role in maintaining the plasticity at glutamatergic synapses (By similarity). Negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway. Acts by competing with calcineurin/PPP3CA for NFAT protein binding, hence preventing NFAT activation by PPP3CA (PubMed:18218901).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. This results in a shorter protein (isoform 2) compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.