Mcub (NM_025779) Mouse Tagged ORF Clone
CAT#: MR205198
- TrueORF®
Ccdc109b (Myc-DDK-tagged) - Mouse coiled-coil domain containing 109B (Ccdc109b)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_025779" in other vectors (4)
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USD 198.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Mcub |
Synonyms | 9030408N13Rik; Ccdc109b; MCUb |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR205198 representing NM_025779
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGGAGCTCTGTCCGGCAGGAGGATGCTCCCGAGCGGGCTATGTCTGGGTCGGTGGCAGCTGCTGC GGACGATCCGAGCCCGCGGCCGCGGGGATCCCAGGGAGCTGCCATCCACACCCCAGGTTTTATGTATGAA ACTCTATGGAAATCCAAAATACCACCAAGCACTCCATTATGGGACAGTGGAGCCACAGGATGAAATAACA GTTACTTATAAACACGGCCTTCCCTTGGTAACCCTCACTCTGCCATCCAGAAAAGAGCGCTGCCAGTTTG TAGTCAAGCCGATGTTGTCGACAGTAGGCTCATTCCTGCAGGACTTACAGAATGAGGATAAAGGCATCAA AACTGCAGCCATCATCACGGCAGATGGCAGTGAAATTCCAGCTTCAACATTGATGGACACTTTGCTAATG ACAGATTTTAAGCTCATCATTAACAAACTACGGTATGATATACGGTGTCACAAGAAAGAAGAGCCAAGTG GAGAGCATATGACTGAGTTGGAAAACACCAAGTCCTTGGTTCACAGACTATTCACTATCCTGCATTTAGA GGAGATCCAGAAGAGACGAGAGCGCCATCTGATGGCGAAGATCGACCACCTGCAGGAACAGCTGCGGCCC TTGGAGCAGGTCAAAGCTGCAATAGAAGCCCGGTCGGAAGCCAACACCAGTGGTCTCCTGTGGGCCGGCT TAGCTCTGCTCTCCGTGCAGGGCGGGGCGCTGGCCTGGCTTACTTGGTGGGTGTACTCCTGGGATATCAT GGAGCCAGTTACCTTCTTCCTGTCGTTTGCGAATTCCATCGTCTTTTTTGCATACTTCATCATAACCCGG CAGAACTACACATACTCATCCCTCCGGAGCAGGCAGTTTCTTCAGTTCTTCCACAAGAAATCGCAGCGGC GGTGTTTTGATGTGGAACAATACAACAAGCTAAAGGAAGACCTTGCTGAGGCTACAGAATCCCTGGAAAG CGTGCGCCGCTCCCTCCGCTTGCGAATCCAAGGAGAGGAAGCCAGCGAGAAGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205198 representing NM_025779
Red=Cloning site Green=Tags(s) MPGALSGRRMLPSGLCLGRWQLLRTIRARGRGDPRELPSTPQVLCMKLYGNPKYHQALHYGTVEPQDEIT VTYKHGLPLVTLTLPSRKERCQFVVKPMLSTVGSFLQDLQNEDKGIKTAAIITADGSEIPASTLMDTLLM TDFKLIINKLRYDIRCHKKEEPSGEHMTELENTKSLVHRLFTILHLEEIQKRRERHLMAKIDHLQEQLRP LEQVKAAIEARSEANTSGLLWAGLALLSVQGGALAWLTWWVYSWDIMEPVTFFLSFANSIVFFAYFIITR QNYTYSSLRSRQFLQFFHKKSQRRCFDVEQYNKLKEDLAEATESLESVRRSLRLRIQGEEASEKN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_025779 |
ORF Size | 1035 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025779.3, NP_080055.2 |
RefSeq Size | 1332 bp |
RefSeq ORF | 1038 bp |
Locus ID | 66815 |
UniProt ID | Q810S1 |
Cytogenetics | 3 G3 |
MW | 40.2 kDa |
Gene Summary | Negatively regulates the activity of MCU, the mitochondrial inner membrane calcium uniporter, and thereby modulates calcium uptake into the mitochondrion. Does not form functional calcium channels by itself. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC210439 | Ccdc109b (untagged) - Mouse coiled-coil domain containing 109B (Ccdc109b), (10ug) |
USD 686.00 |
|
MG205198 | Ccdc109b (tGFP-tagged) - Mouse coiled-coil domain containing 109B (Ccdc109b) |
USD 886.00 |
|
MR205198L3 | Lenti ORF clone of Ccdc109b (Myc-DDK-tagged) - Mouse coiled-coil domain containing 109B (Ccdc109b) |
USD 986.00 |
|
MR205198L4 | Lenti ORF clone of Ccdc109b (mGFP-tagged) - Mouse coiled-coil domain containing 109B (Ccdc109b) |
USD 986.00 |
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