Mcub (NM_025779) Mouse Tagged ORF Clone

CAT#: MG205198

Ccdc109b (tGFP-tagged) - Mouse coiled-coil domain containing 109B (Ccdc109b)

Product Images

Circular map for MG205198

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mcub
• Synonyms: 9030408N13Rik; Ccdc109b; MCUb
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205198 representing NM_025779
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGGAGCTCTGTCCGGCAGGAGGATGCTCCCGAGCGGGCTATGTCTGGGTCGGTGGCAGCTGCTGC
  GGACGATCCGAGCCCGCGGCCGCGGGGATCCCAGGGAGCTGCCATCCACACCCCAGGTTTTATGTATGAA
  ACTCTATGGAAATCCAAAATACCACCAAGCACTCCATTATGGGACAGTGGAGCCACAGGATGAAATAACA
  GTTACTTATAAACACGGCCTTCCCTTGGTAACCCTCACTCTGCCATCCAGAAAAGAGCGCTGCCAGTTTG
  TAGTCAAGCCGATGTTGTCGACAGTAGGCTCATTCCTGCAGGACTTACAGAATGAGGATAAAGGCATCAA
  AACTGCAGCCATCATCACGGCAGATGGCAGTGAAATTCCAGCTTCAACATTGATGGACACTTTGCTAATG
  ACAGATTTTAAGCTCATCATTAACAAACTACGGTATGATATACGGTGTCACAAGAAAGAAGAGCCAAGTG
  GAGAGCATATGACTGAGTTGGAAAACACCAAGTCCTTGGTTCACAGACTATTCACTATCCTGCATTTAGA
  GGAGATCCAGAAGAGACGAGAGCGCCATCTGATGGCGAAGATCGACCACCTGCAGGAACAGCTGCGGCCC
  TTGGAGCAGGTCAAAGCTGCAATAGAAGCCCGGTCGGAAGCCAACACCAGTGGTCTCCTGTGGGCCGGCT
  TAGCTCTGCTCTCCGTGCAGGGCGGGGCGCTGGCCTGGCTTACTTGGTGGGTGTACTCCTGGGATATCAT
  GGAGCCAGTTACCTTCTTCCTGTCGTTTGCGAATTCCATCGTCTTTTTTGCATACTTCATCATAACCCGG
  CAGAACTACACATACTCATCCCTCCGGAGCAGGCAGTTTCTTCAGTTCTTCCACAAGAAATCGCAGCGGC
  GGTGTTTTGATGTGGAACAATACAACAAGCTAAAGGAAGACCTTGCTGAGGCTACAGAATCCCTGGAAAG
  CGTGCGCCGCTCCCTCCGCTTGCGAATCCAAGGAGAGGAAGCCAGCGAGAAGAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205198 representing NM_025779
  Red=Cloning site Green=Tags(s)
  
  MPGALSGRRMLPSGLCLGRWQLLRTIRARGRGDPRELPSTPQVLCMKLYGNPKYHQALHYGTVEPQDEIT
  VTYKHGLPLVTLTLPSRKERCQFVVKPMLSTVGSFLQDLQNEDKGIKTAAIITADGSEIPASTLMDTLLM
  TDFKLIINKLRYDIRCHKKEEPSGEHMTELENTKSLVHRLFTILHLEEIQKRRERHLMAKIDHLQEQLRP
  LEQVKAAIEARSEANTSGLLWAGLALLSVQGGALAWLTWWVYSWDIMEPVTFFLSFANSIVFFAYFIITR
  QNYTYSSLRSRQFLQFFHKKSQRRCFDVEQYNKLKEDLAEATESLESVRRSLRLRIQGEEASEKN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025779
• ORF Size: 1035 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025779.3, NP_080055.2
• RefSeq Size: 1332 bp
• RefSeq ORF: 1038 bp
• Locus ID: 66815
• UniProt ID: Q810S1
• Cytogenetics: 3 G3
• Gene Summary: Negatively regulates the activity of MCU, the mitochondrial inner membrane calcium uniporter, and thereby modulates calcium uptake into the mitochondrion. Does not form functional calcium channels by itself. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways.[UniProtKB/Swiss-Prot Function]