Gstt1 (NM_008185) Mouse Tagged ORF Clone

CAT#: MR202953

Gstt1 (Myc-DDK-tagged) - Mouse glutathione S-transferase, theta 1 (Gstt1)

Product Images

Circular map for MR202953

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gstt1
• Synonyms: AI255817; Gstt1-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR202953 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTCTGGAGCTGTACCTGGATCTGCTGTCGCAGCCCTGTCGCGCCATTTATATCTTCGCCAAGAAGA
  ACAATATCCCGTTCCAGATGCACACGGTGGAGCTGCGCAAGGGTGAGCACCTCAGCGATGCGTTTGCCCG
  GGTGAACCCCATGAAGAGGGTACCAGCCATGATGGATGGTGGCTTCACCCTGTGTGAGAGTGTGGCTATC
  TTGCTCTACCTGGCACACAAGTATAAGGTTCCTGACCACTGGTACCCCCAAGACCTGCAGGCTCGTGCTC
  GTGTAGACGAGTACCTGGCATGGCAGCATACGGGCCTTCGGAGAAGCTGCCTCAGGGCCCTGTGGCATAA
  GGTGATGTTCCCTGTTTTCCTTGGTGAGCAAATACCTCCTGAAACACTGGCAGCCACGTTGGCAGAACTG
  GATGTTAACCTACAGGTGCTTGAAGACAAGTTCCTCCAGGACAAAGACTTCCTTGTTGGGCCCCACATCT
  CCCTGGCCGACTTGGTGGCCATCACAGAGCTGATGCATCCTGTAGGTGGTGGCTGCCCAGTCTTTGAAGG
  GCATCCCAGGCTGGCTGCATGGTACCAGCGAGTGGAGGCAGCTGTGGGGAAGGACCTCTTCCGGGAAGCC
  CATGAAGTCATCCTGAAGGTGAAGGACTGTCCCCCTGCTGACCTCATCATAAAGCAGAAGCTGATGCCCA
  GAGTGCTGGCAATGATCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR202953 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVLELYLDLLSQPCRAIYIFAKKNNIPFQMHTVELRKGEHLSDAFARVNPMKRVPAMMDGGFTLCESVAI
  LLYLAHKYKVPDHWYPQDLQARARVDEYLAWQHTGLRRSCLRALWHKVMFPVFLGEQIPPETLAATLAEL
  DVNLQVLEDKFLQDKDFLVGPHISLADLVAITELMHPVGGGCPVFEGHPRLAAWYQRVEAAVGKDLFREA
  HEVILKVKDCPPADLIIKQKLMPRVLAMIQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008185
• ORF Size: 723 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008185.3
• RefSeq Size: 1049 bp
• RefSeq ORF: 723 bp
• Locus ID: 14871
• UniProt ID: Q64471
• Cytogenetics: 10 38.58 cM
• MW: 27.4 kDa
• Gene Summary: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Also binds steroids, bilirubin, carcinogens and numerous organic anions. Has dichloromethane dehalogenase activity.[UniProtKB/Swiss-Prot Function]