Ier2 (NM_010499) Mouse Tagged ORF Clone
CAT#: MR202538
- TrueORF®
Ier2 (Myc-DDK-tagged) - Mouse immediate early response 2 (Ier2)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_010499" in other vectors (4)
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USD 198.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Ier2 |
Synonyms | AI317238; Ch1; Pip92 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR202538 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGTACAGAAAGAAGCGCAGCGCATCATGACTCTGTCGGTATGGAAGATGTACCACTCTCGCATGC AGCGAGGTGGCTTGCGACTCCACCGGAGTCTGCAGCTGTCCCTCGTTATGCGCAGCGCTCGAGAGGTCTA CCTCTCAGCCAAGGTAGAAGCCCACCAGCCCGAGTTCCCGCCATCCCGCAGGGCTCTTGACCCTCGCCTG CACCCGCCGCGGGAAGCCGAAGTTGCAGTGGAAGTAGCGTCCCCCGAAGCCGTGCAGCCTCCGGAGCCCA TGGATACGCAAGAGGAAGTGCTGCGAGTCCAGGAGACCCCTGCGCTCTGTGACCCGCCCCCCGCTAGAGT CAGCCGCAAGCGCCGGAGCAGCAGCGATTTGAGCGACGGTAGTGATGCCGGACTGGTACCAAGCAAGAAG GCCCGTCTAGAAGAGGTGGAGGGGGAGGCGACGTCGGAGGTTCCCGATCGCCTGCAGCTTCCTCCGGCAC AAAGCGAAGGTGCCTTCCCTAACCTCGCCCGCGTCCTCCAAAGGCGCTTCTCCAGTCTCCTGAACTGTGG ACCCGCCGTGCCCCCGCCGACGCCCCCCACGTGCGAGGCCAAGCCAGCCTGCCGCCCGGCCGACAATATG CTCAACGTGCTGGTGCGAGCTGTGGTGGCCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202538 protein sequence
Red=Cloning site Green=Tags(s) MEVQKEAQRIMTLSVWKMYHSRMQRGGLRLHRSLQLSLVMRSAREVYLSAKVEAHQPEFPPSRRALDPRL HPPREAEVAVEVASPEAVQPPEPMDTQEEVLRVQETPALCDPPPARVSRKRRSSSDLSDGSDAGLVPSKK ARLEEVEGEATSEVPDRLQLPPAQSEGAFPNLARVLQRRFSSLLNCGPAVPPPTPPTCEAKPACRPADNM LNVLVRAVVAF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_010499 |
ORF Size | 666 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010499.4, NP_034629.3 |
RefSeq Size | 1541 bp |
RefSeq ORF | 666 bp |
Locus ID | 15936 |
UniProt ID | P17950 |
Cytogenetics | 8 41.02 cM |
MW | 24.5 kDa |
Gene Summary | DNA-binding protein that seems to act as a transcription factor (By similarity). Involved in the regulation of neuronal differentiation, acts upon JNK-signaling pathway activation and plays a role in neurite outgrowth in hippocampal cells (By similarity). May mediate with FIBP FGF-signaling in the establishment of laterality in the embryo (By similarity). Promotes cell motility, seems to stimulate tumor metastasis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208026 | Ier2 (untagged) - Mouse immediate early response 2 (Ier2), (10ug) |
USD 300.00 |
|
MG202538 | Ier2 (tGFP-tagged) - Mouse immediate early response 2 (Ier2) |
USD 500.00 |
|
MR202538L3 | Lenti ORF clone of Ier2 (Myc-DDK-tagged) - Mouse immediate early response 2 (Ier2) |
USD 600.00 |
|
MR202538L4 | Lenti ORF clone of Ier2 (mGFP-tagged) - Mouse immediate early response 2 (Ier2) |
USD 600.00 |
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