Ier2 (NM_010499) Mouse Tagged ORF Clone

CAT#: MG202538

Ier2 (tGFP-tagged) - Mouse immediate early response 2 (Ier2)

Product Images

Circular map for MG202538

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ier2
• Synonyms: AI317238; Ch1; Pip92
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG202538 representing NM_010499
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGTACAGAAAGAAGCGCAGCGCATCATGACTCTGTCGGTATGGAAGATGTACCACTCTCGCATGC
  AGCGAGGTGGCTTGCGACTCCACCGGAGTCTGCAGCTGTCCCTCGTTATGCGCAGCGCTCGAGAGGTCTA
  CCTCTCAGCCAAGGTAGAAGCCCACCAGCCCGAGTTCCCGCCATCCCGCAGGGCTCTTGACCCTCGCCTG
  CACCCGCCGCGGGAAGCCGAAGTTGCAGTGGAAGTAGCGTCCCCCGAAGCCGTGCAGCCTCCGGAGCCCA
  TGGATACGCAAGAGGAAGTGCTGCGAGTCCAGGAGACCCCTGCGCTCTGTGACCCGCCCCCCGCTAGAGT
  CAGCCGCAAGCGCCGGAGCAGCAGCGATTTGAGCGACGGTAGTGATGCCGGACTGGTACCAAGCAAGAAG
  GCCCGTCTAGAAGAGGTGGAGGGGGAGGCGACGTCGGAGGTTCCCGATCGCCTGCAGCTTCCTCCGGCAC
  AAAGCGAAGGTGCCTTCCCTAACCTCGCCCGCGTCCTCCAAAGGCGCTTCTCCAGTCTCCTGAACTGTGG
  ACCCGCCGTGCCCCCGCCGACGCCCCCCACGTGCGAGGCCAAGCCAGCCTGCCGCCCGGCCGACAATATG
  CTCAACGTGCTGGTGCGAGCTGTGGTGGCCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG202538 representing NM_010499
  Red=Cloning site Green=Tags(s)
  
  MEVQKEAQRIMTLSVWKMYHSRMQRGGLRLHRSLQLSLVMRSAREVYLSAKVEAHQPEFPPSRRALDPRL
  HPPREAEVAVEVASPEAVQPPEPMDTQEEVLRVQETPALCDPPPARVSRKRRSSSDLSDGSDAGLVPSKK
  ARLEEVEGEATSEVPDRLQLPPAQSEGAFPNLARVLQRRFSSLLNCGPAVPPPTPPTCEAKPACRPADNM
  LNVLVRAVVAF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010499
• ORF Size: 663 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010499.4, NP_034629.3
• RefSeq Size: 3302 bp
• RefSeq ORF: 666 bp
• Locus ID: 15936
• UniProt ID: P17950
• Cytogenetics: 8 41.02 cM
• Gene Summary: DNA-binding protein that seems to act as a transcription factor (By similarity). Involved in the regulation of neuronal differentiation, acts upon JNK-signaling pathway activation and plays a role in neurite outgrowth in hippocampal cells (By similarity). May mediate with FIBP FGF-signaling in the establishment of laterality in the embryo (By similarity). Promotes cell motility, seems to stimulate tumor metastasis (By similarity).[UniProtKB/Swiss-Prot Function]