Washc3 (NM_026070) Mouse Tagged ORF Clone

CAT#: MR201887

Ccdc53 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 53 (Ccdc53), transcript variant 1

Product Images

Circular map for MR201887

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Washc3
• Synonyms: 2900091E11Rik; 5730495F03Rik; Ccdc53
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR201887 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGAGGATGGGCTTCCTCTCATGGGGTCAGGCATAGACCTGACCAAGGTGCCAGCTATTCAACAGA
  AAAGAACCGTGGCCTTTCTAAACCAGTTTGTGGTGCACACTGTGCAGTTTCTCAACCGGTTTTCCGCAGT
  TTGTGAGGAGAAACTGGCAGACCTTTCTCTCCGGATTCAGCAAATTGAAACAACTCTCAATATTTTAGAT
  GCAAAGCTGTCATCTATCCCAGGCCTAGAGGACGTCACAGTTGAAGTGTCTCCTTTAAATGTCACTGCTG
  TCACAAACGGATCACATTCTGAAACCACTTCGGAGCAAACGCAGCAGAATAGCACACAAGACTCTGGAGC
  ACAGGAGAGTGAAGCGCCATCAGAAAATGTCCTGACCGTAGCCAAGGATCCGCGGTATGCCAGATACCGC
  AAGATGGTTCAAGTGGGTGTTCCAGTGATGGCAATCAGAGACAAGATGATCTCAGAAGGACTGGACCCGG
  AGCTACTGGAGAAGCCAGATGCCCCGGTGCCAAATGGCGAAAGCAAAAGAGCCGTAGAAGAAAGTTCAGA
  TAGTGACTCTTCCTTCAGTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR201887 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDEDGLPLMGSGIDLTKVPAIQQKRTVAFLNQFVVHTVQFLNRFSAVCEEKLADLSLRIQQIETTLNILD
  AKLSSIPGLEDVTVEVSPLNVTAVTNGSHSETTSEQTQQNSTQDSGAQESEAPSENVLTVAKDPRYARYR
  KMVQVGVPVMAIRDKMISEGLDPELLEKPDAPVPNGESKRAVEESSDSDSSFSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026070
• ORF Size: 585 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026070.1, NM_026070.2, NM_026070.3, NP_080346.1
• RefSeq Size: 1048 bp
• RefSeq ORF: 585 bp
• Locus ID: 67282
• UniProt ID: Q9CR27
• Cytogenetics: 10 C1
• MW: 21.1 kDa
• Gene Summary: Acts at least in part as component of the WASH core complex whose assembly at the surface of endosomes seems to inhibit WASH nucleation-promoting factor (NPF) activity in recruiting and activating the Arp2/3 complex to induce actin polymerization, and which is involved in regulation of the fission of tubules that serve as transport intermediates during endosome sorting.[UniProtKB/Swiss-Prot Function]