AGR2 Rabbit Polyclonal Antibody

CAT#: TA337169

Rabbit Polyclonal AG-2 Antibody

Product Images

Western Blot: AG-2/AGR2 Antibody TA337169 - Analysis using the Azide Free version of TA337169. Detection of AGR2 using AGR2 antibody. MCF7 (A), Caco-2 (B) and human stomach lysate (C) probed with AGR2 antibody at 1:100. were used for this test. The higher molecular weight band of variable intensity at ~30 kDa is uncharacterized and may represent a form of AGR2.

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  Immunohistochemistry-Paraffin: AG-2/AGR2 Antibody TA337169 - Analysis using the Azide Free version of TA337169. Staining of human adenocarcinoma of the breast stained with AGR2 antibody (2 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

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  Immunohistochemistry-Paraffin: AG-2/AGR2 Antibody TA337169 - Analysis using the Azide Free version of TA337169. Staining of human adenocarcinoma of the colon stained with AGR2 antibody (2-5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

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  Simple Western: AG-2/AGR2 Antibody TA337169 - Analysis using the Azide Free version of TA337169. Simple Western lane view shows a specific band for AGR2 in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Specifications

Product Data

• Applications: ICC/IF, IHC, IP, Simple Western, WB
• Recommended Dilution: Immunohistochemistry-Paraffin: 1:100~, Knockdown Validated, Immunoprecipitation: 1:10-1:500, Western Blot: 1:100, Immunohistochemistry-Frozen: 1:20-1:1000, Simple Western: 1:50, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: This antibody was developed against synthetic peptides corresponding to amino acids 55-72 of human AGR2.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: anterior gradient 2, protein disulphide isomerase family member
• Database Link:
  NP_006399
  Entrez Gene 23795 MouseEntrez Gene 10551 Human
  UniProt ID O95994
• Background: hAG-2 and hAG-3 are recently discovered human homologues of the secreted Xenopus laevis proteins XAG-1/2 (AGR1 and AGR2) that are expressed in the cement gland, an ectodermal organ in the head associated with anteroposterior fate determination during early development (1). Although the roles of hAG-2 and hAG-3 in mammalian cells are unknown, both proteins share a high degree of protein sequence homology and lie adjacent to one another on chromosome 7p21.
• Synonyms: AG2; GOB-4; HAG-2; HEL-S-116; PDIA17; XAG-2

Reference Data

• Protein Families: Secreted Protein