Ntrk3 Rabbit Polyclonal Antibody

CAT#: TA319458

Rabbit polyclonal anti-TrkCT1 antibody

Product Images

Western blot using affinity purified anti-TrkCT1 to detect over-expressed TrkCT1 in HEK293 cells (Lane 2, arrowhead). Lane 1 is a non-transfected control. Cell extracts were resolved by electrophoresis and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:3,000 dilution. Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.

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  Mouse cortex lysate was immunoprecipitated with anti-TrkCT1 antibody and further blotted with affinity purified anti-TrkCT1. Lane 1 is wild-type cortex lysate, Lane 2 is Tamalin knock-out cortex lysate, and Lane 3 is TrkCT1 knock-out cortex lysate. The membrane was probed with the primary antibody at a 1:6,000 dilution. Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.

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  Western blot using affinity purified anti-TrkCT1 to detect endogenous TrkCT1 in mouse cortex lysate (Lane 1). Lane 2 is TrkCT1 knock-out cortex lysate. Cell extracts were resolved by electrophoresis and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:6,000 dilution. Personal Communication, V. Coppola, CCRNCI, Frederick, MD.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:5,000 - 1:20,000, WB: 1:1,000 - 1:10,000, IP: 1 ug
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of mouse TrkCT1 protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: neurotrophic tyrosine kinase, receptor, type 3
• Database Link:
  NP_032772
  Entrez Gene 4916 HumanEntrez Gene 29613 RatEntrez Gene 18213 Mouse
  UniProt ID Q6VNS1
• Synonyms: gp145(trkC); GP145-TrkC; OTTHUMP00000192915; Trk-C; TRKC
• Note: This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. TrkCT1, also named neurotrophic tyrosine kinase receptor type 3 (Ntrk3) non-catalytic isoform 2, is a non-catalytic isoform of TrkC, the high affinity receptor for Neurotrophin-3 (NT-3). This isoform lacks the kinase domain that is responsible for signaling by the full-length isoform. TrkCT1 is the product of an alternative splicing of Ntrk3 that leaves the extracellular and transmembrane domains intact but includes a shorter intracellular domain encoded by exons 13b and 14b. Recent studies indicate that the short cytoplasmic tail binds the scaffold protein Tamalin in a ligand-dependent manner and further activates the Arf6–Rac1 signaling pathway (Esteban et al., 2006).