TRIM5 alpha (TRIM5) Rabbit Polyclonal Antibody

CAT#: TA306150

Rabbit Polyclonal TRIM5 gamma Antibody

Product Images

Western blot analysis of TRIM5 gamma expression in human bladder (A) and colon (B) cell lysate with TRIM5 gamma antibody at 2 ug /ml.

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  Immunohistochemistry of TRIM5g in human bladder tissue with TRIM5g antibody at 2 ug/ml.

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  Immunofluorescence of TRIM5 gamma in human bladder tissue with TRIM5 gamma antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 2 ug/mL, ICC: 2 ug/mL, IF: 20 ug/mL
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: TRIM5 gamma antibody was raised against a synthetic peptide corresponding to 15 amino acids near the carboxy-terminus of human TRIM5 gamma.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: tripartite motif containing 5
• Database Link:
  NP_149083
  Entrez Gene 85363 Human
  UniProt ID Q9C035
• Background: TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region (1). TRIM5 has five alternately spliced isoforms, the longest of which is the ? variant which also contains a carboxy-terminal B30.2 (SPRY) domain (1). While a function has not yet been assigned to TRIM5gamma, it is known that expression of TRIM5gamma variants from humans, rhesus monkeys, and African green monkeys enable resistance to infection by various retroviruses including HIV-1 (2,3), albeit at differing efficiencies. Furthermore, the TRIM5gamma isoform appears to serve as a scaffold for the assembly of endogenous BTBD1 and BTBD2 proteins and also exhibits autoubiquitination activity in a RING finger- and UbcH5B-dependent manner (4).
• Synonyms: RNF88; TRIM5alpha