P4HA1 Goat Polyclonal Antibody

CAT#: TA302527

Goat Polyclonal Antibody against P4HA1

Product Images

TA302527 (0.2ug/ml) staining of HEK293 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:32,000. WB: 0.1-0.1µg/ml.
• Reactivities: Human (Expected from sequence similarity: Mouse, Rat, Dog)
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-DFARKDEPDAFKE, from the internal region of the protein sequence according to NP_000908.2; NP_001017962.1.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 64596 Da
• Gene Name: prolyl 4-hydroxylase subunit alpha 1
• Database Link:
  NP_000908
  Entrez Gene 18451 MouseEntrez Gene 64475 RatEntrez Gene 479242 DogEntrez Gene 5033 Human
  UniProt ID P13674
• Background: This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]
• Synonyms: P4HA

Reference Data

• Protein Families: Druggable Genome, P450
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways