Myeloperoxidase (MPO) Mouse Monoclonal Antibody [Clone ID: 2C7]

CAT#: SM1475F

Myeloperoxidase (MPO) mouse monoclonal antibody, clone 2C7, FITC

Conjugation: Unconjugated FITC

USD 520.00

5 Days*

    • 100 ug

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Product Data
Clone Name 2C7
Applications FC
Recommended Dilution Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 106 cells in 100 µl.
Membrane permeabilisation is required for this application.
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Human myeloperoxidase.
Spleen cells from immunised mice were fused with cells of the mouse X63 AG8-653 myeloma cell line.
Specificity This antibody recognises myeloperoxidase (MPO), an important component of azurophilic granules in neutrophils.
Negative Species: Rat.
Formulation PBS, pH 7.4
Label: FITC
State: Liquid purified IgG fraction
Stabilizer: 1% BSA
Preservative: 0.09% Sodium Azide
Label: Fluorescein Isothiocyanate Isomer 1
Concentration lot specific
Purification Affinity Chromatography on Protein G
Conjugation FITC
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Gene Name myeloperoxidase
Background Human myeloperoxidase (MPO) is a dimeric protein composed of two heavy subunits (53 kDa) and two light subunits (15 kDa). Each MPO molecule contains two prosthetic porphyrins which play an important role in the catalytic cycle. Molecular weights for MPO isoforms from pools of normal human samples range from 114,000 to 140,000 daltons reflecting a heterogeneous mixture of isoforms when assayed under non-reducing conditions of SDS-PAGE. Often MPO from a single donor will yield a homogenous preparation reflecting a single isoform. The carbohydrate component of MPO, consisting of mannose, glucose and N-acetylglucosamine residues is 2.5%. MPO is inhibited by azide and other compounds. MPO is stored in primary granules of neutrophils and serves as a bactericidal agent in that MPO catalyzes the production of hypochlorous acid (HOCl), a powerful oxidant. HOCl is derived from chloride ion (Cl-) and hydrogen peroxide (H2O2). In a number of inflammatory situations, MPO is released into the extracellular matrix where its measurement can be used as an indication of neutrophil activation.
Synonyms MPO
Reference Data

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