Helicobacter pylori Rabbit Polyclonal Antibody

CAT#: DP016

Helicobacter pylori rabbit polyclonal antibody, Purified

Product Images

Formalin fixed paraffin embedded human stomach infected with H. pylori and stained with H. pylori antibody (DP016/DP016-05)

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Paraffin Embedded Sections: Use a dilution of 1/50-1/100 in an ABC method (30 minutes at RT).
  Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
  Recommended Positive Control: infected stomach.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Total lysate of H. pylori
• Specificity: This antibody reacts with the whole H. pylori.
  The antibody stains the bacteria present on the surface of the epithelium or in the cytoplasm of the epithelial cells.
  Cellular localization: cytoplasmic.
• Formulation: State: Purified
  State: Liquid purified Ig fraction containing Sodium Azide as preservative.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcimona and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. More recently this bacterium has also been implicated with a number of vascular disorders including heart disease. It is not clear how H. pylori is transmitted or why some patients become symptomatic while others do not. The bacteria are most likely spread from person to person through fecal-to-oral or oral-to-oral routes. Possible environmental reservoirs include contaminated water sources. Serological tests that measure specific H. pylori IgG antibodies can determine if a person has been infected although these methods do have drawbacks and limitations.
• Synonyms: H. pylori