GSC Mouse Monoclonal Antibody [Clone ID: OTI1D7]

CAT#: CF500023

Carrier-free (BSA/glycerol-free) Goosecoid mouse monoclonal antibody, clone OTI1D7 (formerly 1D7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GSC (Cat# RC209586, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GSC(Cat# TA500023). Positive lysates LY406285 (100ug) and LC406285 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GSC monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GSC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500023)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GSC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500023)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-GSC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500023)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GSC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500023)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-GSC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500023)

• HEK293T cells transfected with either RC209586 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GSC antibody (TA500023), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1D7
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~1000, IHC 1:50, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 107-257 of human GSC (NP_776248) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28.0 kDa
• Gene Name: goosecoid homeobox
• Database Link:
  NP_776248
  Entrez Gene 145258 Human
  UniProt ID P56915
• Background: Goosecoid is a member of the bicoid subfamily of the paired (PRD) homeobox family of proteins. It acts as a transcription factor and may be autoregulatory. A similar protein in mice plays a role in craniofacial and rib cage development during embryogenesis.
• Synonyms: SAMS

Reference Data

• Protein Families: Transcription Factors